A5033872198- Retinal Diseases and Treatments
- Photoacoustic and Ultrasonic Imaging
- Retinal and Optic Conditions
- Optical Coherence Tomography Applications
- Retinal Imaging and Analysis
- Nanoplatforms for cancer theranostics
- Glaucoma and retinal disorders
- Retinal Development and Disorders
- Retinal and Macular Surgery
- Extracellular vesicles in disease
- Ocular Diseases and Behçet’s Syndrome
- Intraocular Surgery and Lenses
- Ocular and Laser Science Research
- Ultrasound and Hyperthermia Applications
- Corneal Surgery and Treatments
- Optical Imaging and Spectroscopy Techniques
- Cerebral Venous Sinus Thrombosis
- Corneal surgery and disorders
- Thermography and Photoacoustic Techniques
- Dermatologic Treatments and Research
- Advanced Fluorescence Microscopy Techniques
- Angiogenesis and VEGF in Cancer
- Ocular Oncology and Treatments
- Retinopathy of Prematurity Studies
- Cerebrovascular and Carotid Artery Diseases
University of Michigan
2016-2025
Johns Hopkins Medicine
2015-2025
Johns Hopkins University
2014-2025
Michigan United
2016-2024
W.K. Kellogg Foundation
2015-2021
Michigan Medicine
2021
Visual Sciences (United States)
2016-2021
Associated Research (United States)
2020
Pfizer (United States)
2020
Syneos Health (India)
2020
Abstract Colloidal gold nanoparticles (GNPs) serve as promising contrast agents in photoacoustic (PA) imaging, yet their utility is limited due to absorption peak the visible window overlapping with that of hemoglobin. To overcome such limitation, this report describes an ultrapure chain-like nanoparticle (CGNP) clusters a redshift wavelength at 650 nm. The synthesized CGNP show excellent biocompatibility and photostability. These are conjugated arginine-glycine-aspartic acid (RGD) peptides...
Objective: To systematically evaluate the effects of laser beam size, power, and pulse duration 1 to 100 milliseconds on characteristics ophthalmoscopically visible retinal coagulation lesions.Methods: A 532-nm Nd:YAG was used irradiate 36 retinas in Dutch Belt rabbits with sizes 66, 132, 330 µm.Lesions were clinically graded minute after placement, their size measured by digital imaging, depth assessed histologically at different time points.Results: Retinal lesion increased linearly power...
To systematically assess the changes in retinal morphology during healing of photocoagulation lesions various clinical grades.Rabbits were irradiated with a 532-nm Nd:YAG laser beam diameter 330 microm at surface, power 175 mW, and pulse durations between 5 100 ms. Retinal clinically graded 1 minute after placement as invisible, barely visible, light, moderate, intense, very rupture assessed histologically six time points from hour to 4 months.At all durations, width decreased over time. At...
We developed a photoacoustic ocular imaging device and demonstrated its utility in the deeper layers of eye including retina, choroid, optic nerve. Using safe laser intensity, system was able to visualize blood distribution an enucleated pig's living rabbit. Ultrasound images, which were simultaneously acquired, overlaid on images eye's anatomy. Such may be used future for early detection improved management neovascular diseases, wet age-related macular degeneration proliferative diabetic...
Photoacoustic microscopy (PAM) is an emerging imaging technology that can non-invasively visualize ocular structures in animal eyes. This report describes integrated multimodality system combines PAM, optical coherence tomography (OCT), and fluorescence (FM) to evaluate angiogenesis larger High-resolution vivo was performed live rabbit eyes with vascular endothelial growth factor (VEGF)-induced retinal neovascularization (RNV). The results demonstrate our RNV both albino pigmented rabbits...
Most reported photoacoustic ocular imaging work to date uses small animals, such as mice and rats, the eyeball sizes of which are less than one-third those humans, posing challenges for clinical translation.Here we developed a novel integrated microscopy (PAM) optical coherence tomography (OCT) system dual-modality chorioretinal larger rabbits.The has quantified lateral resolutions 4.1 µm 3.8 (OCT), axial 37.0 4.0 at focal plane objective.Experimental results in living rabbits demonstrate...
Ocular drug delivery remains a grand challenge due to the complex structure of eye. Here, we introduce unique platform ocular through integration silicon nanoneedles with tear-soluble contact lens. The can penetrate into cornea in minimally invasive manner and then undergo gradual degradation over course months, enabling painless long-term sustained drugs. lens fit variety corneal sizes quickly dissolve tear fluid within minute, an initial burst release anti-inflammatory We demonstrated...
Purpose.: Müller glia respond to retinal injury by a reactive gliosis, but only rarely do mammalian glial cells re-enter the cell cycle and generate new neurons. In nonmammalian retina, however, act as stem/progenitor cells. Here, we tested function of Wnt signaling in postinjury focusing on its ability influence dedifferentiation, proliferation, neurogenesis. Methods.: A 532 nm frequency doubled neodymium-doped yttrium aluminum garnet (Nd:YAG) laser was used create light burns retina...
High-quality, wide-field retinal imaging is a valuable method for screening preventable, vision-threatening diseases of the retina. Smartphone-based cameras hold promise increasing access to imaging, but variable image quality and restricted field view can limit their utility. We developed clinically tested smartphone-based system that addresses these challenges with automation-assisted imaging.The was designed improve smartphone by combining automated fixation guidance, photomontage,...
Multimodal imaging with photoacoustic microscopy (PAM) and optical coherence tomography (OCT) can be an effective method to evaluate the choroidal retinal microvasculature. To improve efficiency for visualizing capillaries, colloidal gold nanoparticles (AuNPs) have been applied as a multimodal contrast agent both OCT PAM by taking advantage of strong scattering absorption AuNPs due their surface plasmon resonance. Ultra-pure were fabricated femtosecond laser ablation, capped polyethylene...
Currently, available gold nanoparticles (GNPs) typically accumulate in the liver and spleen, leading to concerns for their long-term biosafety. To address this long-standing problem, ultraminiature chain-like nanoparticle clusters (GNCs) are developed. Via self-assembly of 7-8 nm GNP monomers, GNCs provide redshifted optical absorption scattering contrast near-infrared window. After disassembly, turn back GNPs with a size smaller than renal glomerular filtration cutoff, allowing excretion...
In laser retinal photocoagulation, short (<20 ms) pulses have been found to reduce thermal damage the inner retina, decrease treatment time, and minimize pain. However, safe therapeutic window (defined as ratio of power for producing a rupture that mild coagulation) decreases with shorter exposures. To quantify extent heating maximize window, computational model millisecond photocoagulation was developed. Optical attenuation 532-nm light in ocular tissues measured, including pigment...
CNS neurons change their connectivity to accommodate a changing environment, form memories, or respond injury. Plasticity in the adult mammalian retina after injury disease was thought be limited restructuring resulting abnormal retinal anatomy and function. Here we report that restore normal function Patches of photoreceptors rabbit were destroyed by selective laser photocoagulation, leaving inner (bipolar, amacrine, horizontal, ganglion cells) intact. Photoreceptors located outside damaged...
Ischemia‐induced hypoxia is a critical complication in retinal diseases, leading to significant vision impairment and blindness due disrupted blood flow oxygen delivery. Currently, there no effective method assess levels extravascular tissue. Traditional detection methods, such as oxygen‐sensitive microelectrodes, magnetic resonance imaging, oximetry, have limitations including invasiveness, low spatial resolution, lack of real‐time monitoring. Herein, noninvasive proposed by utilizing...
Sustained-release ocular drug delivery systems with minimal invasiveness are critical for managing eye diseases that cause blindness. An innovative platform is presented painless and long-term sustained utilizing controllably biodegradable silicon nanoneedles (Si NNs) conjugated bevacizumab (Bev) integrated into a tear-soluble subconjunctival patch. The biocompatible patch facilitates easy application in the area of rapid dissolution less than one minute upon contact tear film sclera,...
The early history of retinal laser therapy began with the observation solar blindness, leading to development various technologies for treating diseases. first photocoagulation machine was developed in 1956, and subsequent advancements introduced ruby argon lasers. While conventional theories posited that damage an indispensable prerequisite therapeutic effects, emergent insights highlight non-damaging mechanisms, such as photostimulation- self-repair-related mechanisms. Novel therapies,...