A5063813102- Retinal Development and Disorders
- Retinal and Macular Surgery
- Retinal Diseases and Treatments
- Neuroscience and Neural Engineering
- Intraocular Surgery and Lenses
- Glaucoma and retinal disorders
- Photoreceptor and optogenetics research
- Retinal Imaging and Analysis
- Retinal and Optic Conditions
- Mobile Health and mHealth Applications
- 3D Printing in Biomedical Research
- Corneal Surgery and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Botulinum Toxin and Related Neurological Disorders
- Ocular Oncology and Treatments
- Tissue Engineering and Regenerative Medicine
- Ocular Disorders and Treatments
- Vascular Malformations Diagnosis and Treatment
- Social Policies and Healthcare Reform
- Advanced Proteomics Techniques and Applications
- Ocular and Laser Science Research
- Corneal surgery and disorders
- Nerve injury and regeneration
Knappschaftsklinikum Saar
2019-2024
Fraunhofer Institute for Biomedical Engineering
2018-2023
University of Bonn
2011-2022
National University of Singapore
2020-2021
National University Hospital
2021
Singapore National Eye Center
2016-2021
National Eye Institute
2015-2017
Universitäts-Augenklinik Bonn
2008-2016
National Institutes of Health
2015
University Hospital Bonn
2009-2013
Considerable progress has been made in testing stem cell-derived retinal pigment epithelium (RPE) as a potential therapy for age-related macular degeneration (AMD). However, the recent reports of oncogenic mutations induced pluripotent cells (iPSCs) underlie need robust manufacturing and functional validation clinical-grade iPSC-derived RPE before transplantation. Here, we developed mutation-free iPSCs from three AMD patients differentiated them into iPSC-RPE patches on biodegradable...
To present the outcome of a consecutive series patients who had foveal choroidal neovascularization (fCNV) in age-related macular degeneration (AMD) and were treated with subretinal surgery combined simultaneous transplantation autologous retinal pigment epithelial (RPE) cells.Patients fCNV not eligible for laser or photodynamic therapy included study. They underwent membrane excision RPE cells. Eyes alone served as control. Tests best corrected visual acuity far near Early Treatment...
Transplantation of the retinal pigment epithelium (RPE) is being developed as a cell-replacement therapy for age-related macular degeneration. Human embryonic stem cell (hESC) and induced pluripotent (iPSC)-derived RPE are currently translating toward clinic. We introduce adult human (hRPESC) an alternative source. Polarized monolayers hRPESC-derived grown on polyester (PET) membranes had near-native characteristics. Trephined pieces PET were transplanted subretinally in rabbit, large-eyed...
To systematically assess the changes in retinal morphology during healing of photocoagulation lesions various clinical grades.Rabbits were irradiated with a 532-nm Nd:YAG laser beam diameter 330 microm at surface, power 175 mW, and pulse durations between 5 100 ms. Retinal clinically graded 1 minute after placement as invisible, barely visible, light, moderate, intense, very rupture assessed histologically six time points from hour to 4 months.At all durations, width decreased over time. At...
Recent trials of retinal pigment epithelium (RPE) transplantation for the treatment disorders such as age-related macular degeneration have been promising. However, limitations existing strategies include uncertain survival RPE cells delivered by cell suspension and inherent risk uncontrolled proliferation in vitreous cavity. Human stem cell-derived (hRPESC-RPE) can rescue vision a rat model dystrophy survive rabbit retina at least 1 month. The present study placed hRPESC-RPE monolayers...
Purpose.: To develop a surgical technique for the subretinal implantation of cell carriers suitable transplantation cultured retinal pigment epithelium (RPE) in preclinical animal model. Methods.: Cell were porous 10-μm-thick polyester membranes. A custom-made shooter instrument consisted 20-gauge metallic nozzle with nonstick plunger. Fetal human RPE cultures used vitality assessment during handling. Transvitreal without was performed 31 rabbits after vitrectomy. Fourteen implants...
Mapping of proteins involved in normal eye functions is a prerequisite to identify pathological changes during disease processes. We therefore analysed the proteome human vitreous by applying in-depth proteomic screening technologies. For ethical reasons samples were obtained vitrectomy from "surrogate patients" with epiretinal gliosis that considered constitute only negligible vitreoretinal changes. applied different protein prefractionation strategies including liquid phase isoelectric...
In this study, we investigated the suitability of ultrathin and porous polyimide (PI) membrane as a carrier for subretinal transplantation human embryonic stem cell (hESC) -derived retinal pigment epithelial (RPE) cells in rabbits. The vivo effects hESC-RPE were analyzed by suspension injection into Royal College Surgeons (RCS) rats. Rat eyes with electroretinography (ERG) histology. After analyzing surface permeability properties PI, PI transplantations without performed rabbits followed...
Ozurdex, a novel dexamethasone (DEX) implant, is released by drug delivery system into the vitreous cavity. We analyzed mechanical release aperture of applicator, obtained real-time recordings using high-speed camera and performed kinematic analysis DEX application.Experimental study.: The application intravitreal implants (6 mm length, 0.46 diameter; 700 μg mass, 0.0012 g total implant mass) was recorded (500 frames per second) in water (Group A: n = 7) or B: filled tanks. Kinematic...
Purpose: To study alginate- and hyaluronic acid–based hydrogels in vitro as vitreous substitutes. Methods: Biopolymeric based on high-molecular alginate (0.5% 1.0%) acid (1.0% Healaflow) were compared with extracted human bodies silicone oil (SIL-5000) regarding their optical properties (refractive index, transmission) viscoelastic characteristics (storage modulus G′, loss G″). The cytotoxic (metabolic activity, apoptosis) antiproliferative profiles determined using cultured fibroblasts,...
To determine the viscoelasticity of human vitreous bodies and its changes with age in order to benefit understanding therapy vitreoretinal diseases.In a postmortem study, 190 were extracted from 33- 92-year-old donors, analyzed regard their viscoelastic properties via dynamic mechanical analyses, compared bovine porcine vitreous. Postmortem intervals donor-related parameters examined as potential influencing viscoelasticity. Dynamic moduli different hyaluronic acid (HA) solutions well...
The lack of suitable animal models for (dry) age-related macular degeneration (AMD) has hampered therapeutic research into the disease, so far. In this study, pigmented rats and mice were systematically injected with various doses sodium iodate (SI). After injection, retinal structure visual function non-invasively characterized over time to obtain in-depth data on suitability these studying experimental therapies degenerative diseases, such as dry AMD.SI was tail vein (i.v.) using a series...
Age related macular degeneration (AMD), retinitis pigmentosa, and other RPE diseases are the most common causes for irreversible loss of vision in adults industrially developed countries. transplantation appears to be a promising therapy, as it may replace dysfunctional RPE, restore its function, thereby vision. Here we describe method transplanting cultured monolayer on scaffold into subretinal space (SRS) rabbits. After vitrectomy xenotransplants were delivered SRS using custom made...
Purpose: Delivery of Advanced Therapy Medicinal Products to the submacular space is increasingly evolving into a therapeutic modality. Cell replacement for age-related macular degeneration (AMD) and gene therapy RPE65 are recent successful examples. Herein, nonhuman primate (NHP) model was used investigate surgical means detach macula. Methods: Sixteen eyes 13 healthy macaques underwent 25-gauge vitrectomy subretinal injection balanced salt solution monitored by microscope-integrated...
<i>Background:</i> To evaluate the influence of surface topography on proliferation retinal pigment epithelium (RPE) by comparing nanofibrillar and smooth substrates. <i>Methods:</i> Electrospun polyamide nanofibers (EPN) are an engineered mimicking native basement membranes. Commonly used plastic (polystyrene, PS) glass substrates have a topography. All were analyzed scanning electron microscopy. RPE cultures established from fetal adult donors. Growth curves above...
Abstract Purpose To study immunogenic properties of human embryonic stem cell–derived retinal pigment epithelium ( hESC ‐ RPE ) and to evaluate subretinal xenotransplantation on porous polyethylene terephthalate PET in rabbits. Methods Human ESC cells were characterized by morphology, transepithelial electrical resistance TER ), protein expression photoreceptor outer segment phagocytosis vitro . Expression major histocompatibility complex MHC proteins was assessed conventionally or xeno‐free...
Human pluripotent stem cells (hPSCs) provide a promising cell source for retinal replacement therapy but often lack standardized production and live-cell shipment logistics as well rigorous analyses of surgical procedures transplantation in the delicate macula area. We have previously established xeno- feeder cell-free system hPSC differentiated pigment epithelial (RPE) cells, herein, novel immunosuppressed non-human primate (NHP) model with disrupted ocular immune privilege is presented...
Cell therapy is a promising treatment for retinal pigment epithelium (RPE)-associated eye diseases such as age-related macular degeneration. Herein, selective microsecond laser irradiation targeting RPE cells was used minimally invasive, large-area removal in preparation delivery of cell therapeutics.Ten rabbit eyes were exposed to pulses 8, 12, 16, and 20 µs duration (wavelength, 532 nm; top-hat beam profile, 223 × µm²). Post-irradiation changes assessed with fluorescein angiography (FA),...