A5065574048- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Parasites and Host Interactions
- Immune Cell Function and Interaction
- Galectins and Cancer Biology
- Helminth infection and control
- Reproductive System and Pregnancy
- Coccidia and coccidiosis research
- Immunotherapy and Immune Responses
- Synthesis and Biological Evaluation
- T-cell and B-cell Immunology
- Viral Infections and Immunology Research
- Pregnancy and Medication Impact
- Tryptophan and brain disorders
- Immune cells in cancer
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Toxin Mechanisms and Immunotoxins
- Calcium signaling and nucleotide metabolism
- Cardiomyopathy and Myosin Studies
- Vector-borne infectious diseases
- Long-Term Effects of COVID-19
- Pregnancy and preeclampsia studies
- Parasitic infections in humans and animals
- Cancer Mechanisms and Therapy
Universidad Nacional de Córdoba
2014-2024
Research Centre in Biological Chemistry of Córdoba
2001-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2014-2024
University of California, Los Angeles
2008
CIUDAD
2001
Abstract The role of transcription factors in B cell survival and differentiation has been delineated during the last years. However, little is known about intermediate signals intracellular pathways that control these events. In this study, we provide evidence both vitro vivo, showing galectin-3 (Gal-3), a β-galactoside-binding protein, critical mediator survival. Although Gal-3 not expressed resting cells from normal mice, its expression markedly induced after activation with stimuli such...
Abstract Galectin‐1 has been implicated in regulating T‐cell survival, function, and Th1/Th2 balance several mouse models, though the molecular cellular basis of its immuno‐modulatory activity not completely elucidated. Therefore, we examined galectin‐1 expression within differentiated murine Th1 Th2 subsets. While recombinant specifically bound to both subsets, T cells expressed distinct combinations galectin‐1‐reactive epitopes were differentially responsive exposure. Indeed, more...
Chagas disease causes a cardiac illness characterized by immunoinflammatory reactions leading to myocardial fibrosis and remodeling. The development of Chronic Cardiomyopathy (CCC) in some patients while others remain asymptomatic is not fully understood, but dysregulated inflammatory responses are implicated. Aryl hydrocarbon receptor (AhR) plays crucial role regulating inflammation. Certain tryptophan (Trp) metabolites have been identified as AhR ligands with regulatory functions.
Indoleamine 2,3-dioxigenase (IDO) is an inflammatory cytokine-inducible rate-limiting enzyme of the tryptophan (Trp) catabolism, which involved in inhibition intracellular pathogen replication as well immunomodulation. Here we demonstrated effect IDO-dependent Trp catabolism on Trypanosoma cruzi resistance to acute infection. Infection with T. resulted systemic activation IDO. The blocking IDO activity vivo impaired infection and exacerbated parasite load infection-associated pathology. In...
Dendritic cells (DC) have the potential to control outcome of autoimmunity by modulating immune response. In this study, we tested ability Fasciola hepatica total extract (TE) induce tolerogenic properties in CpG-ODN (CpG) maturated DC, then evaluate therapeutic these diminish inflammatory response collagen induced arthritis (CIA). DBA/1J mice were injected with TE plus CpG treated DC (T/C-DC) pulsed bovine II (CII) between two immunizations CII and clinical scores CIA determined. The levels...
The complete repertoire of proteins with immunomodulatory activity in Fasciola hepatica (Fh) has not yet been fully described. Here, we demonstrated that Fh total extract (TE) reduced LPS-induced DC maturation, and the ability to induce allogeneic responses. After TE fractionating, a fraction lower than 10 kDa (F<10 kDa) was able maintain properties modulate pro- anti-inflammatory cytokine production induced by LPS. In addition, or F<10 treatment decreased immature stimulate responses novo...
Because of their plasticity and central role in orchestrating immunity tolerance, DCs can respond to pregnancy-specific signals, thus promoting the appropriate immune response order support pregnancy. Here, we show that glycoprotein (PSG1a), major variant PSG released into circulation during pregnancy, targets differentiate a subset with unique phenotype function. This semi-mature is able secrete IL-6 TGF-β. PSG1a also affected maturation DCs, preventing up-regulation some costimulatory...
Acute infection with Trypanosoma cruzi is characterized by multiple manifestations of immunosuppression both cellular and humoral responses. B cells isolated at the acute stage have shown marked impairment in their response to polyclonal activators vitro. The present work aims studying cell compartment context T. provide evidence for activation, spontaneous apoptosis arrest cycle upon mitogenic stimulation as a mechanism underlying hyporesponse. We found that from acutely infected mice,...
Abstract The control of B cell expansion has been thought to be solely regulated by T lymphocytes. We show in this study that Trypanosoma cruzi infection induces up-regulation both Fas and ligand (FasL) molecules on cells renders them susceptible cell-B killing (referred as fratricide throughout paper) mediated via Fas/FasL. Moreover, vivo administration anti-FasL blocking mAb we demonstrate Fas-mediated apoptosis is an ongoing process during parasitic infection. also provide evidence have...
Resistance to Trypanosoma cruzi infection is dependent on a rapid induction of Th1-type and CD8+ T cell responses that should be promptly balanced prevent immunopathology. T. cruzi-infected B6 mice are able control parasite replication but show limited expansion Foxp3+regulatory (Treg) cells results in the accumulation effector immune development acute liver pathology. AhR ligand-activated transcription factor promotes Treg suppression pro-inflammatory cytokine production dendritic cells,...
Abstract The range of vaccines developed against severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) provides a unique opportunity to study immunization across different platforms. In single-center cohort, we analyzed the humoral and cellular immune compartments following five disease 2019 (COVID-19) spanning three technologies (adenoviral, mRNA inactivated virus) administered in 16 combinations. For adenoviral inactivated-virus vaccines, heterologous combinations were generally...
Systemic lupus erythematosus (SLE) is a complex autoimmune disease characterized by hyperactive immune system, including activation of autoreactive T and B cells. These studies demonstrate that administration recombinant galectin-1, β-galactose binding protein, to SLE-prone (NZB × NZW) F1 mice reduced lymphocyte activation, inhibited serum anti-double-stranded DNA(dsDNA) IgG antibody production, decreased the incidence proteinuria, increased survival rate. In addition, galectin-1’-treated...
Abstract It has been proposed that pregnancy-specific factors induce the suppression of a specific arm maternal response accompanied by activation nonspecific, innate immune system. The aim this study was to determine whether glycoprotein 1a (PSG1a), major variant PSG polypeptides, is able modulate monocyte/macrophage (Mo) metabolism rgulate T cell and proliferation. Using recombinant form (rec-PSG1a), expressed in mammalian cells with vaccinia-based expression vector, we have demonstrated...
Background 3-Hydroxy Kynurenine (3-HK) administration during the acute phase of Trypanosoma. cruzi infection decreases parasitemia lethally infected mice and improves their survival. However, due to fact that treatment with 3-HK is unable eradicate parasite, together known proapoptotic immunoregulatory properties downstream catabolites, it possible effective by controlling parasite replication, but at same time suppressed protective T cell response before pathogen clearance worsening chronic...
Anaplastic thyroid cancer (ATC) is a highly aggressive type of (TC). Currently, no effective target treatments are available that can improve overall survival, with ATC representing major clinical challenge because its remarkable lethality. Tumor-associated macrophages (TAMs) the most evident cells in ATCs, and their high density correlated poor prognosis. However, mechanisms how TAMs promote progression remain poorly characterized. Here, we demonstrated treatment human monocytes (THP-1...
Abstract Mitochondrial DNA from 141 individuals was typed for diagnostic restriction sites and the 9‐bp region V deletion to examine distribution of founding mtDNA lineage haplotypes in three Amerindian populations (Mataco, Toba, Pilagá) who currently inhabit Argentinian part Gran Chaco. All four lineages were identified tribes population samples studied. Disregarding ethnic or geographic origin, haplogroups B D exhibit high incidence among Chaco inhabitants, whereas A C are present a lower...
During the acute phase of Trypanosoma cruzi infection, macrophages can act as host cells for parasites well effector in early anti-parasitic immune response. Thus, targeting specific signaling pathways could modulate response to restrict parasite replication and instruct an appropriate adaptive Recently, it has become apparent that Wnt pathway exerts immunomodulatory functions during inflammation infection. Here, we tested hypothesis T. activation plays a role modulating...
The production of IL-1-family cytokines such as IL-1β and IL-18 is finely regulated by inflammasome activation after the recognition pathogens associated molecular pattern (PAMPs) danger patterns (DAMPs). However, little known about helminth-derived molecules capable activating inflammasome. In case helminth trematode Fasciola hepatica, secretion different cathepsin L cysteine peptidases (FhCL) crucial for parasite survival. Among these enzymes, L3 (FhCL3) expressed mainly in juvenile or...
Abstract It has been proposed that pregnancy‐specific factors could be responsible for shift the balance of cytokine profiles during maternal immune response from Th1‐type reactivity into a "less‐damaging" Th2‐type reactivity. In present work, we investigated in vivo function human glycoprotein (PSG)1a, major variant PSG polypeptides released thecirculation pregnancy, on modulation innate and adaptive response. For this, BALB/c mice were injected with vaccinia virus‐based vector harboring...