A5026199658- Retinal Diseases and Treatments
- Retinal Development and Disorders
- Protease and Inhibitor Mechanisms
- Retinopathy of Prematurity Studies
- Blood properties and coagulation
- Glaucoma and retinal disorders
- Signaling Pathways in Disease
- Retinal Imaging and Analysis
- Neuroinflammation and Neurodegeneration Mechanisms
- Autophagy in Disease and Therapy
- Cellular transport and secretion
- Retinoids in leukemia and cellular processes
- Adipose Tissue and Metabolism
- Erythrocyte Function and Pathophysiology
- Immunotherapy and Immune Responses
- Blood Coagulation and Thrombosis Mechanisms
- Neurological Disorders and Treatments
- Calpain Protease Function and Regulation
- Growth Hormone and Insulin-like Growth Factors
- Complement system in diseases
- Advanced Glycation End Products research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Antioxidant Activity and Oxidative Stress
- Religious and Theological Studies
- Protein purification and stability
Consejo Nacional de Investigaciones Científicas y Técnicas
2016-2025
Research Centre in Biological Chemistry of Córdoba
2010-2025
Universidad Nacional de Córdoba
2016-2025
Heidelberg University
2025
University Hospital Heidelberg
2025
Universidad Juárez Autónoma de Tabasco
2024
Centro Científico Tecnológico - Tucumán
2022
Departamento de Educación
2018
Centro Científico Tecnológico - Córdoba
2017
Instituto de Investigaciones Agropecuarias
2015
The complete repertoire of proteins with immunomodulatory activity in Fasciola hepatica (Fh) has not yet been fully described. Here, we demonstrated that Fh total extract (TE) reduced LPS-induced DC maturation, and the ability to induce allogeneic responses. After TE fractionating, a fraction lower than 10 kDa (F<10 kDa) was able maintain properties modulate pro- anti-inflammatory cytokine production induced by LPS. In addition, or F<10 treatment decreased immature stimulate responses novo...
Because of their plasticity and central role in orchestrating immunity tolerance, DCs can respond to pregnancy-specific signals, thus promoting the appropriate immune response order support pregnancy. Here, we show that glycoprotein (PSG1a), major variant PSG released into circulation during pregnancy, targets differentiate a subset with unique phenotype function. This semi-mature is able secrete IL-6 TGF-β. PSG1a also affected maturation DCs, preventing up-regulation some costimulatory...
Inflammation and oxidative nitrosative stress are involved in the pathogenesis of proliferative retinopathies (PR). In PR, a loss balance between pro-angiogenic anti-angiogenic factors favors secretion vascular endothelial growth factor (VEGF). This change results alterations blood–retinal barrier, with extravasation plasma proteins such as α2-macroglobulin (α2M) gliosis Müller glial cells (MGCs, MIO-M1). It is well known that MGCs play important roles healthy sick retinas, including PR....
Measuring co-localization of different types molecules is essential to understand molecular organization in biological systems. The pair cross-correlation (PCC) function computed from two-color microscopy images provides a measure between differently labeled molecules. Here, we compute theoretical expression for the PCC two using two-dimensional Gaussian distributions as effective point spread functions single Through our analytical calculations, provide quantitative description case...
Vascular endothelial growth factor (VEGF) inhibition is currently the first-line therapy for various retinal vascular disorders, however there a strong need to develop novel therapies target other molecules involved in angiogenic process. In addition well-known antibiotic properties, Doxycycline (DXC) has versatile non-antibiotic therefore, our goal was evaluate effect of DXC on matrix metalloproteinase-2 (MMP-2) as potential therapeutic alternative neovascularization (NV), using and glial...
Inflammation and oxidative stress are involved in Proliferative Retinopathies (PR). Müller glial cells (MGCs) microglia play pivotal roles pathological neovascularization (NV) neurodegeneration PR. Nitro-fatty acids important electrophilic signaling mediators with anti-inflammatory antioxidant properties. Herein, our goal was to evaluate the cytoprotective effect of nitro-oleic acid (NO2-OA) on neurons, MGCs a mouse model oxygen-induced retinopathy (OIR). NO2-OA induced vascular regrowth...
Abstract Macrophages under certain stimuli induce matrix metalloproteinase 9 (MMP‐9) expression and protein secretion through the activation of MAPK‐ERK NF‐κB signaling pathways. Previously, we demonstrated that activated α 2 ‐macroglulin (α M*) interaction with its receptor low‐density lipoprotein receptor‐related 1 (LRP1) induces macrophage proliferation mediated by MAPK‐ERK1/2. In present work, examined whether M*/LRP1interaction could MMP‐9 production in J774 Raw264.7 macrophage‐derived...
Hypoxia and hypoxia-reoxygenation are frequently developed through the course of many retinal diseases different etiologies. Müller glial cells (MGCs), together with microglia astrocytes, participate firstly in response to injury later repair tissue damage. New pharmacological strategies tend modulate MGCs ability induce angiogenesis gliosis order accelerate recovery stage. In this article, we investigated variation autophagy flux under hypoxia during 4 h, employing both gas culture chamber...
In retinal proliferative diseases, Müller glial cells (MGCs) acquire migratory abilities. However, the mechanisms that regulate this migration remain poorly understood. addition, disorders associated with enhanced activities of matrix metalloprotease 2 (MMP-2) and MMP-9 also present increased levels protease inhibitor α2-macroglobulin (α2M) its receptor, low-density lipoprotein receptor-related protein 1 (LRP1). work, we investigated whether activated form α2M, α2M*, LRP1 are involved MGC...
// Magali E. Ridano 1 , Paula V. Subirada María C. Paz Valeria Lorenc 1,5 Juan Stupirski 2 Ana L. Gramajo 3 José D. Luna Diego O. Croci 2,6 Gabriel A. Rabinovich 2,4 and Sánchez Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), Consejo Nacional Científicas y Técnicas (CONICET), Departamento Clínica, Facultad Ciencias Químicas, Universidad Córdoba, Argentina Laboratorio Inmunopatología, Instituto Biología Medicina Experimental (IBYME), CONICET, Buenos Aires, Privado Ojos...
Abstract Insulin-like Growth Factor-1 (IGF-1) is involved in the normal development and survival of retinal cells. Low-density lipoprotein Receptor-related Protein-1 (LRP1) plays a key role on regulation several membrane proteins, such as IGF-1 receptor (IGF-1R). In brain astrocytes, LRP1 interact with IGF-1R glucose transporter type 1 (GLUT1), regulating uptake these Although GLUT1 expressed Müller Glial Cells (MGCs), its not clear yet. Here, we investigated whether modulates traffic to...
Although it is known that Müller cells express the glial fibrillary acidic protein (GFAP) in response to acute retinal damage, regulatory mechanism not completely understood. α(2)-Macroglobulin (α(2)M) and its receptor, low-density lipoprotein receptor-related 1 (LRP1), have also been found injured retinas. Herein, authors examined involvement of α(2)M/LRP1 system GFAP expression using vitro vivo experimental models.Using Western blot analysis immunocytochemistry, evaluated effect α(2)M* on...
In ischemic proliferative retinopathies, Müller glial cells (MGCs) acquire migratory abilities. However, the mechanisms that regulate this migration remain poorly understood. addition, disorders associated with enhanced activities of matrix metalloproteinases (MMPs) also involve insulin-like growth factor (IGF)-1 participation. Therefore, main interest work was to investigate IGF-1 effect on extracellular proteolytic activity in MGCs.Cell culture supernatants and cell lysates human MGC line...
Abstract Low‐density lipoprotein receptor‐related protein 1 (LRP1) is an endocytic receptor, which binds and internalizes diverse ligands such as activated α 2 ‐macroglobulin (α M*). LRP1 promotes intracellular signaling, downstream mediates cellular proliferation migration of different types cells, including macrophages. Unlike the LDL expression not sensitive to cholesterol levels but appears be responsive insulin. It has been previously demonstrated that insulin increases cell surface...
Abstract Hemin is an erythropoietic inductor capable of inducing autophagy in erythroid-like cell lines. Low-density lipoprotein receptor-related protein 1 (LRP1) a transmembrane receptor involved wide range cellular processes, such as proliferation, differentiation, and metabolism. Our aim was to evaluate whether LRP1 responsible for hemin activity K562 cells, with the results demonstrating three-fold increase gene expression levels (P-values &lt;0.001) when assessed by quantitative...
Climatic droplet keratopathy (CDK) is an acquired corneal disease characterized by progressive scarring of the cornea. In several diseases, matrix metalloproteinases (MMPs) are upregulated during degradation epithelial and stromal tissues. We investigated levels, degree activation molecular forms MMP-2, MMP-9, MMP-8 MMP-13 their tissue inhibitors TIMP-1 TIMP-2 in tear fluid patients with CDK.Seventeen CDK 10 controls living Argentine Patagonia received a complete eye examination, MMPs...
Hypoxia is one of the main insults in proliferative retinopathies, leading to neovascularization and neurodegeneration. To maintain homeostasis, neurons require efficient degradation recycling systems. Autophagy participates retinal cell death, but it also a survival mechanism. Here, we analysed role autophagy at three characteristic time periods oxygen-induced retinopathy (OIR) mouse model determined if its modulation can improve vascular non-vascular alterations. Experiments were performed...