Cytotoxic T lymphocytes (CTLs) recognize peptide antigens associated with cell surface major histocompatibility complex (MHC) molecules. The identification of tumor cell-derived peptides capable eliciting anti-tumor CTL responses would enable the design antigen-specific immunotherapies. Our strategy to identify such potentially therapeutic relies on selecting high-affinity MHC binders from known tumor-associated antigens. These are subsequently tested for their ability induce CTLs killing...

CD45R+ and CDw29+ CD4+ T cells are widely regarded as separate functionally defined cell lineages. The work described here indicates that they represent maturation stages within the same differentiation pathway. Purified populations of or CD8+ cells, after stimulation with PHA, lose surface expression CD45R (Lp220) gain an increased density CDw29 (4B4). Clonal analysis demonstrated individual lost acquired time in culture. This effect was selective for high Mr 220-kDa form T200 (CD45)...

Abstract Intraocular inflammatory diseases are a common cause of severe visual impairment and blindness. In this study, we investigated the immunoregulatory role galectin-1 (Gal-1), an endogenous lectin found at sites T cell activation immune privilege, in experimental autoimmune uveitis (EAU), Th1-mediated model retinal disease. Treatment with rGal-1 either early or late during course interphotoreceptor retinoid-binding protein-induced EAU was sufficient to suppress ocular pathology,...

Glycoproteins are potentially important biomarkers of disease and therapeutic targets. In particular, the N-linked glycoproteins a focus interest as they can be found in extracellular environment body fluids. this study, we have sampled tears, fluid epithelial cells covering surface eye, patients with climatic droplet keratopathy (CDK) using tears unaffected normal for comparison. Prefractionation tear sample used hydrazide-resin capture method, previously N-glycosylated peptides were then...

Resistance to Trypanosoma cruzi infection is dependent on a rapid induction of Th1-type and CD8+ T cell responses that should be promptly balanced prevent immunopathology. T. cruzi-infected B6 mice are able control parasite replication but show limited expansion Foxp3+regulatory (Treg) cells results in the accumulation effector immune development acute liver pathology. AhR ligand-activated transcription factor promotes Treg suppression pro-inflammatory cytokine production dendritic cells,...

Retinal tears complicating the course of a posterior vitreous detachment (PVD) may be unique or multiple, and when multiple they occur simultaneously subsequently at different moments in evolution PVD. The purpose our study was to analyze prevalence subsequent retinal (SRT) patients with PVD, identify possible risk factors for SRT. One hundred seventy six eyes 165 consecutive that presented one more symptomatic minimum follow-up 12 months, were retrospectively evaluated. primary outcome...

Solar damage due to ultraviolet radiation (UVR) is implicated in the development of two proliferative lesions ocular surface: pterygium and pinguecula. Pterygium pinguecula specimens were collected, along with adjacent healthy conjunctiva specimens. RNA was extracted sequenced. Pairwise comparisons made differentially expressed genes (DEGs). Computational methods used for analysis. Transcripts from 18,630 identified. Comparison subgroups uncovered evidence genomic instability associated...

Expression of murine CCL21 by dermal lymphatic endothelial cells (LEC) has been demonstrated to be one the most important steps in Langerhans cell emigration from skin. Previously, our group and others have found that this chemokine is up-regulated different human inflammatory skin diseases mediated diverse specific immune responses. This study was carried out investigate involvement after challenge with irritant agents responsible for inducing Irritant Contact Dermatitis (ICD). Eleven...

Abstract The characteristics of T and B lymphocyte profile specificity repertoire were compared in patients with Waidenstrom's macroglobulinemia (WM), IgM monoclonal gammopathy undetermined significance (IgM MGUS), multiple myeloma (MM), age‐matched normal subjects. Patients MM had both significantly reduced frequency number sIg + (surface Ig) cells, whereas WM MGUS a but numbers cells circulation as detected capping assay. was distinguished by the large peripheral blood (PBL) pool that...

Abstract Objective To describe the functional and structural characteristics of cornea in healthy Guinea pigs. Animals studied Healthy male female pigmented albino pigs ( Caviaporcellus ) aged 3–5 months old were used. Procedures The animals’ corneas underwent different vivo studies including: slit‐lamp biomicroscopy, fluorescein staining (FS), break‐up time test (BUT), confocal microscopy pachymetry. also histopathologically with light microscopy, immunohistochemistry transmission electron...

Climatic droplet keratopathy (CDK) is a degenerative disease of the cornea with possible involvement from matrix metalloproteinases (MMPs). Therefore, authors investigated histologic distribution, levels, and molecular forms MMP-2 MMP-9, as well tear fluid levels MMPs cytokines in CDK patients. They additionally examined UV-B-irradiation effect on production gelatinases by human corneal epithelial (HCE) cell culture model.Tears were collected 20 unrelated individuals (10 10 controls)....

We carried out a seroepidemiologic survey to define the prevalence of human T cell lymphotropic virus types 1/2 (HTLV-1/2) infections among aboriginal populations from isolated regions northern Argentina and Amazon region Peru. Antibodies against HTLV were measured with agglutination tests confirmed by an immunofluorescence assay (IFA) Western blotting. Five (6.94%) 72 samples Tobas Indians in positive IFA; two typed as HTLV-1 (2.78%), HTLV-2 one (1.39%) could not be because it had similar...

Recruitment of lymphocytes into the retina and to vitreous during development experimental autoimmune uveitis (EAU) is governed by factors such as state activation inflammatory cells repertoire adhesion molecules expressed local vascular endothelia. alpha4 Integrins their receptors play an important role homing site. In present study, effect alpha4-integrin inhibitor on EAU was investigated.EAU induced either immunizing B10.RIII mice with 161-180 peptide or adoptive transfer...

High molecular mass isoforms of CD45 protein tyrosine phosphatase, predominantly CD45RA, are expressed on naive T cells with increasing density during the first 2 days cellular activation, and subsequently down-regulated concurrent expression low Mr isoform, CD45RO. We show this to be de novo synthesis CD45RA dependent upon both RNA synthesis. Using a probe shown detect mRNA encoding alternatively spliced abc, ab, bc, b exon CD45, was analyzed. Kinetic studies transition from high indicate...

Chemokines are important players in the development of allergic contact dermatitis (ACD). The participation secondary lymphoid tissue chemokine (CCL21) is essential induction disease due to its expression lymphatic vessels and organs. Since there no information about during effector phase ACD, we studied this patients already diagnosed with who were challenged relevant positive negative (control) antigens. All showed a specific antigen-induced immune response characterized by early...

To identify the proteins in corneal droplets of climatic droplet keratopathy (CDK), a disease that results formation on cornea. Progressive accumulation CDK leads to visual loss.Proteomic mass spectrometry specimens was performed after fractionation 4% 20% SDS-polyacrylamide gels. Droplets were derived from two human donors. Immunohistochemistry with antibodies confirm presence identified donor tissues patients and control subjects.Proteomic analyses revealed identification 105 specimens....

Climatic droplet keratopathy (CDK) is an acquired corneal disease characterized by progressive scarring of the cornea. In several diseases, matrix metalloproteinases (MMPs) are upregulated during degradation epithelial and stromal tissues. We investigated levels, degree activation molecular forms MMP-2, MMP-9, MMP-8 MMP-13 their tissue inhibitors TIMP-1 TIMP-2 in tear fluid patients with CDK.Seventeen CDK 10 controls living Argentine Patagonia received a complete eye examination, MMPs...

Climatic droplet keratopathy (CDK) is a degenerative corneal disease of unknown etiology. We described CDK for the first time in Latin America Argentinean Patagonia (El Cuy). A deeper knowledge pathogenic mechanisms will provide new therapeutic strategies. For that reason we investigated prevalence El Cuy and its existence other 3 provinces with similar climate. Patients eyes were examined, habits throughout lives inquired about, serum ascorbate (sAA) was determined. All individuals work...

The objectives of this study were firstly, to compare the immunophenotype patients with monoclonal gammopathy undetermined significance (MGUS) that newly diagnosed, untreated multiple myeloma (Unt. MM). Our second objective was determine which variables might distinguish MGUS and early MM. CD4/CD8 ratio in both patient groups differed significantly from normal as a result decrease proportion CD4 + cells. Similarly, surface immunoglobulin‐positive (Ig ) B cells reduced groups. Also, some...