A5021271372- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Birth, Development, and Health
- RNA modifications and cancer
- Telomeres, Telomerase, and Senescence
- Genomics and Chromatin Dynamics
- DNA and Nucleic Acid Chemistry
- Protein Kinase Regulation and GTPase Signaling
- Monoclonal and Polyclonal Antibodies Research
- Plant Virus Research Studies
- Microbial Inactivation Methods
- Advanced biosensing and bioanalysis techniques
- Chromosomal and Genetic Variations
- Cancer-related Molecular Pathways
- Polyomavirus and related diseases
- RNA regulation and disease
- Genetics, Aging, and Longevity in Model Organisms
- Toxin Mechanisms and Immunotoxins
- RNA Research and Splicing
- Kruppel-like factors research
Université de Strasbourg
2012-2024
Biotechnologie et Signalisation Cellulaire
2012-2024
École Supérieure de Biotechnologie de Strasbourg
2003-2019
Centre National de la Recherche Scientifique
1998-2016
École Normale Supérieure - PSL
2005-2007
Institut de génétique et de biologie moléculaire et cellulaire
1996-2003
Inserm
1988-2003
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
1988-1995
Institut de Chimie
1990-1994
University of Chicago
1994
S1 mapping on theVASl structural gene indicates the existence of two classes transcripts initiating at distinct in-frame translation start codons.The longer class VASl initiates upstream both ATG codons located 138 base pairs away and shorter downstream first ATG.A mutation that destroys AUG long message results in respiratory deficiency but does not affect viability.Mutation position 139 leads to lethality because methionine codon essential cytoplasmic valyl-tRNA synthetase has been...
The product of adenovirus (Ad) type 5 gene IX (pIX) is known to actively participate in the stability viral icosahedron, acting as a capsid cement. We have previously demonstrated that pIX also transcriptional activator several and cellular TATA-containing promoters, likely contributing transactivation Ad expression program. By extensive mutagenesis, we now delineated functional domains involved each properties: residues 22 26 highly conserved N-terminal domain are crucial for incorporation...
ATF or CRE binding proteins are cellular transcription factors involved in the regulation of adenovirus Ela-responsive and cAMP-inducible promoters. We report isolation from a HeLa cell cDNA library two clones that encode with specific ATF/CRE DNA activity. The differ by 63 bp element which is retained one (ATF-a) deleted other (ATF-aΔ) may correspond to an alternative exon. peptide sequences (483 462 amino acids, respectively) derived each these cDNAs identical, except for additional 21...
We recently isolated three cDNA clones encoding closely related proteins (ATFa1, ATFa2, and ATFa3) that belong to the activating transcription factor-cyclic AMP-responsive element family of cellular factors. Using cotransfection experiments, we showed these mediate transcriptional activation induced by adenovirus E1a 13S mRNA gene product zinc-binding domains present in both conserved region 3 most N-terminal portion ATFa play crucial roles this activity. Reciprocal coimmunoprecipitation...
The host range of polyoma virus is dependent upon the activity its enhancer, which inactive in undifferentiated embryonal carcinoma cells, such as F9 and active after their differentiation. We show here that alpha domain enhancer displays a similar cell-specificity inducibility does whole enhancer. present evidence to determined by balance between activities two factors, PEA2, labile repressor, PEA1, an inducible positive factor we have characterized previously. Changes repressor help...
The VASI gene encoding the valyl-tRNA synthetase from yeast was isolated and sequenced. gene-derived amino acid sequence of found to be 23% homologous Escherichia coli isoleucyl-tRNA synthetase. This is highest level homology reported so far between two distinct aminoacyl-tRNA synthetases indicative an evolutionary relationship these molecules. Within sequences, functional regions could recognized: HIGH region which forms part binding site ATP KMSKS recognized as consensus for 3'-end tRNA...
Various stresses increase disease susceptibility and accelerate aging, increasing evidence suggests that these effects can be transmitted over generation. Epidemiological studies suggest stressors experienced by parents affect the longevity of their offspring, possibly regulating telomere dynamics. Telomeres are elongated telomerase shortened certain as well repeat-containing RNA (TERRA), a transcript. However, mechanism underlying transgenerational is poorly understood. Here, we show TNF-α,...
Telomeres maintain the integrity of chromosome ends and telomere length is an important marker aging. The epidemiological studies suggested that many types stress including psychosocial decrease length. However, it remains unknown how various stresses induce shortening. Here, we report stress-responsive transcription factor ATF7 mediates TNF-α-induced telomerase, enzyme elongates telomeres, are localized on telomeres via interactions with Ku complex. In response to TNF-α, which induced by...
Abstract It has been proposed that pregnancy-specific factors induce the suppression of a specific arm maternal response accompanied by activation nonspecific, innate immune system. The aim this study was to determine whether glycoprotein 1a (PSG1a), major variant PSG polypeptides, is able modulate monocyte/macrophage (Mo) metabolism rgulate T cell and proliferation. Using recombinant form (rec-PSG1a), expressed in mammalian cells with vaccinia-based expression vector, we have demonstrated...
Switching between replicative and translesion synthesis (TLS) DNA polymerases are crucial events for the completion of genomic when replication machinery encounters lesions in template.In eukaryotes, translesional polymerase (Pol) plays a central role accurate bypass cyclobutane pyrimidine dimers, predominant induced by ultraviolet irradiation.Pol deficiency is responsible variant form Xeroderma pigmentosum (XPV) syndrome, characterized predisposition to skin cancer.Here, we show that FF...
Transient transfection of foreign DNA is the most widely used laboratory technique to study gene function and product. However, efficiency depends on many parameters, including quantity quality, methods target cell lines. Here, we describe considerable advantage mRNA electroporation compared conventional DNA-based systems. Indeed, our methodology offers extremely high up 98% regardless line tested. Protein expression takes place a few hours post-transfection lasts over 72 h, but overall,...
We recently isolated three cDNA clones encoding closely related proteins (ATFa1, ATFa2, and ATFa3) that belong to the activating transcription factor-cyclic AMP-responsive element family of cellular factors. Using cotransfection experiments, we showed these mediate transcriptional activation induced by adenovirus E1a 13S mRNA gene product zinc-binding domains present in both conserved region 3 most N-terminal portion ATFa play crucial roles this activity. Reciprocal coimmunoprecipitation...