A5106417138- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Glycosylation and Glycoproteins Research
- Protein purification and stability
- RNA and protein synthesis mechanisms
- Advanced Biosensing Techniques and Applications
- Immunotherapy and Immune Responses
- Fungal and yeast genetics research
- Microbial Inactivation Methods
- Insect Resistance and Genetics
- Endoplasmic Reticulum Stress and Disease
- vaccines and immunoinformatics approaches
- Toxin Mechanisms and Immunotoxins
- Viral Infectious Diseases and Gene Expression in Insects
- T-cell and B-cell Immunology
- Genomics and Chromatin Dynamics
- Gene Regulatory Network Analysis
- Microtubule and mitosis dynamics
- Polysaccharides and Plant Cell Walls
- T-cell and Retrovirus Studies
- Advanced Electron Microscopy Techniques and Applications
- Vector-Borne Animal Diseases
- Molecular Communication and Nanonetworks
- Nuclear Structure and Function
Biotechnologie et Signalisation Cellulaire
2013-2024
Université de Strasbourg
2013-2024
Centre National de la Recherche Scientifique
1998-2024
École Supérieure de Biotechnologie de Strasbourg
2008-2024
Institut de génétique et de biologie moléculaire et cellulaire
2024
Inserm
2023
MorphoSys (Germany)
2007
Ludwig-Maximilians-Universität München
1997-2006
Max Planck Institute of Biochemistry
2005
Max Planck Society
2005
Nanoparticle (NP) surface functionalization with proteins, including monoclonal antibodies (mAbs), mAb fragments, and various peptides, has emerged as a promising strategy to enhance tumor targeting specificity immune cell interaction. However, these methods often rely on complex chemistry suffer from batch-dependent outcomes, primarily due limited control over the protein orientation quantity NP surfaces. To address challenges, novel approach based supramolecular assembly of two peptides is...
Protein translocation across the mitochondrial inner membrane is driven by cycles of binding and release heat shock protein 70 (mtHsp70) in matrix. The peripheral protein, Tim44, recruits mtHsp70 an ATP-dependent manner to import sites. We show that DnaK, closely related Hsp70 Escherichia coli, when targeted matrix yeast mitochondria, interacts a specific with Tim44. interaction is, however, not regulated ATP, DnaK cannot support translocation. used truncated mtHsp70s chimeric proteins...
Multiple myeloma is characterized by clonal expansion of B cells producing monoclonal immunoglobulins or fragments thereof, which can be detected in the serum and/or urine and are ideal target antigens for patient-specific immunotherapies. Using phage particles as immunological carriers, we employed a novel chemically linked idiotype vaccine clinical phase I/II trial including 15 patients with advanced multiple myeloma. Vaccines composed purified paraproteins to were manufactured...
Therapeutic monoclonal antibodies benefit to patients and the conjugation gold nanoparticles (AuNPs) might bring additional activities these macromolecules. However, behavior of conjugate will largely depend on bulkiness AuNP small sizes are moreover preferable for diffusion. Water-soluble thiolate-protected AuNPs having diameters 2-3 nm can be synthesized with narrow polydispersity selectively react incoming organic thiols via a SN2-like mechanism. We therefore mixed thionitrobenzoic acid-...
Summary Expression of the yeast Saccharomyces cerevisiae SRP1 (serine‐rich protein) gene is shown here to be induced both‐ by low temperature and anaerobic growth conditions. We show that expression haem‐dependent; however, haem influence does not operate through action hypoxic‐gene ROX1 repressor. The promoter region displaying stress‐responsive elements restricted its first 551 bp, upstream initiation codon, although an activation site contained in sequences required for full activity. In...
Transient transfection of foreign DNA is the most widely used laboratory technique to study gene function and product. However, efficiency depends on many parameters, including quantity quality, methods target cell lines. Here, we describe considerable advantage mRNA electroporation compared conventional DNA-based systems. Indeed, our methodology offers extremely high up 98% regardless line tested. Protein expression takes place a few hours post-transfection lasts over 72 h, but overall,...
Saccharomyces cerevisiae Gpa2p, the α subunit of a heterotrimeric guanine nucleotide-binding protein (G protein), is involved in regulation vegetative growth and pseudohyphal development. Here we report that Gpa2p also controls sporulation by interacting with regulatory domain Ime2p (Sme1p), kinase essential for entrance meiosis sporulation. Protein-protein interactions between depend on GTP-bound state correlate down-regulation activity vitro. Overexpression inhibits development enables...
Abstract Advances in cryo‐electron microscopy (EM) enable imaging of protein assemblies within mammalian cells a near native state when samples are preserved by cryogenic vitrification. To accompany this progress, specialized EM labelling protocols must be developed. Gold nanoparticles (AuNPs) 2 nm synthesized and functionalized to bind selected intracellular targets inside living human detected vitreous sections. As proof concept, thioaminobenzoate‐, thionitrobenzoate‐coordinated gold on...
We constructed hybrid proteins containing a plant α‐galactosidase fused to various C‐terminal moieties of the hypoxic Srp1p; this allowed us identify cell wall‐bound form Srp1p. showed that last 30 amino acids Srp1p, but not 16, contain sufficient information signal glycosyl‐phosphatidylinositol anchor attachment and subsequent wall anchorage. The was shown be linked by means β1,6‐glucose‐containing side‐chain. Pmt1p enzyme is known as protein‐O‐mannosyltransferase initiates O‐glycosidic...
Alternative splicing and post-translational modifications are processes that give rise to the complexity of proteome. The nuclear ATF7 ATF2 (activating transcription factor) structurally homologous leucine zipper factors encoded by distinct genes. Stress growth activate mainly via sequential phosphorylation two conserved threonine residues in their activation domain. Distinct protein kinases, among which mitogen-activated kinases (MAPK), phosphorylate first on Thr71/Thr53 next Thr69/Thr51...
Abstract Background B cell malignancies are characterized by clonal expansion of cells expressing tumor-specific idiotypes on their surface. These ideal target antigens for an individualized immunotherapy. However, previous idiotype vaccines mostly lacked efficiency due to a low immunogenicity the idiotype. The objective present study was determination feasibility, safety and novel chemically linked phage vaccine. Methods In murine lymphoma 1 model, tumor were particles used as immunological...
Abstract Purpose: The frequently expressed differentiation antigen tyrosinase-related protein-2 (TRP-2) has repeatedly been described as a target of spontaneous cytotoxic T-cell responses in melanoma patients, suggesting that it might be an ideal candidate for T cell–based immunotherapy. As prerequisite immunization, epitopes have to identified. Whereas number HLA class I–presented TRP-2–derived are known, information about II–presented antigenic ligands recognized by CD4+ helper (Th) cells...
Immunotoxins are emerging candidates for cancer therapeutics. These biomolecules consist of a cell-targeting protein combined to polypeptide toxin. Associations both entities can be achieved either chemically by covalent bonds or genetically creating fusion proteins. However, chemical agents affect the activity and/or stability conjugate proteins, and additional purification steps often required isolate final from unwanted byproducts. As they suffer low solubility yield. In this report, we...
Monitoring the assembly of macromolecules to design entities with novel properties can be achieved either chemically creating covalent bonds or by noncovalent connections using appropriate structural motifs. In this report, two self-associating peptides (named K3 and E3) that originate from p53 tetramerization domain were developed as tools for highly specific heterotetramerization biomolecules. The pairing/coupling preferences E3 first evaluated molecular modeling data confirmed circular...
In this paper, the potential of microelectronic design methods in synthetic biology is analyzed. Synthetic an emerging domain, which aims to combine and genetics knowledge with engineering order new biological systems. The argument illustrated by a case study on specific biodevice, modeled simulated using three different languages: MATLAB-Simulink two hardware description languages (VHDL-AMS VHDL). Whereas simulations give equivalent results, HDL modeling more relevant approach according way...
The human T-cell leukemia virus type 1 (HTLV-1)-encoded Tax protein activates transcription from the long terminal repetition via association with host cellular factors. In this study, we searched for proteins that interact and modulate its activity by using yeast two-hybrid system. One of strongest interactors was found to be identical TRBP, which previously shown bind RNA encoded Tat response element immunodeficiency 1. Interactions are demonstrated Escherichia coli-expressed in vitro...
The transcription factor ATF7 undergoes multiple post-translational modifications, each of which has distinct effects upon function. Here, we show that phosphorylation on residue Thr112 exclusively occurs during mitosis, and is excluded from the condensed chromatin. Both processes are CDK1/cyclin B dependent. Using a transduced neutralizing monoclonal antibody directed against epitope in living cells, could demonstrate protects endogenous protein degradation, while it no effect displacement...
The homogeneous labeling of antibodies and their fragments is a critical step for the generation robust probes used in immuno-detection applications. To date, numerous chemical, genetic peptide-based site-specific coupling methods have been developed. Among these methods, co-assembling peptide-tags one most straightforward versatile solutions. Here, we describe nanobodies through use two co-associating peptides tags, E3 K3, originating from tetramerization domain p53. These K3-tags provide...