A5020320735- Advanced biosensing and bioanalysis techniques
- Cell Adhesion Molecules Research
- Molecular Sensors and Ion Detection
- Monoclonal and Polyclonal Antibodies Research
- Glioma Diagnosis and Treatment
- RNA Interference and Gene Delivery
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Advancements in Transdermal Drug Delivery
- Sex work and related issues
- Advanced Drug Delivery Systems
- HER2/EGFR in Cancer Research
- Sexuality, Behavior, and Technology
- Advanced Nanomaterials in Catalysis
- Advanced Biosensing Techniques and Applications
- Urologic and reproductive health conditions
- Ovarian cancer diagnosis and treatment
- Microtubule and mitosis dynamics
- Blood properties and coagulation
- Nanoparticle-Based Drug Delivery
- HIV, Drug Use, Sexual Risk
- Molecular Biology Techniques and Applications
- Analytical Chemistry and Sensors
- Gold and Silver Nanoparticles Synthesis and Applications
- Cancer, Hypoxia, and Metabolism
Université de Strasbourg
2019-2024
Centre National de la Recherche Scientifique
2019-2024
Laboratoire de Biophotonique et Pharmacologie
2019-2024
Universidade do Porto
2016-2017
Rede de Química e Tecnologia
2016-2017
Förster resonance energy transfer (FRET) is essential in optical materials for light-harvesting, photovoltaics, and biosensing, but its operating range fundamentally limited by the radius of ≈5 nm. In this work, FRET between fluorescent organic nanoparticles (NPs) studied order to break limit. The donor acceptor NPs are built from charged hydrophobic polymers loaded with cationic dyes bulky counterions. Their surface functionalized DNA control surface-to-surface distance. It found that...
Nucleic acid aptamers are often referred to as chemical antibodies. Because they possess several advantages, like their smaller size, temperature stability, ease of modification, lack immunogenicity and toxicity, lower cost production, promising tools for clinical applications. Aptamers against cell surface protein biomarkers particular interest cancer diagnosis targeted therapy. In this study, we identified characterized RNA targeting cells expressing integrin α5β1. This αβ heterodimeric...
Local pharmacies play a vital role in delivering essential healthcare services Talavera, Nueva Ecija, particularly providing access to medicines and basic health consultations. Despite their importance, these establishments face several operational challenges such as increasing competition from large pharmaceutical chains, financial constraints, regulatory compliance issues, evolving customer expectations. This study aims assess the current landscape of local by identifying key obstacles...
Therapeutic monoclonal antibodies benefit to patients and the conjugation gold nanoparticles (AuNPs) might bring additional activities these macromolecules. However, behavior of conjugate will largely depend on bulkiness AuNP small sizes are moreover preferable for diffusion. Water-soluble thiolate-protected AuNPs having diameters 2-3 nm can be synthesized with narrow polydispersity selectively react incoming organic thiols via a SN2-like mechanism. We therefore mixed thionitrobenzoic acid-...
Abstract The performance of fluorescence immunostaining is physically limited by the brightness organic dyes, whereas labeling with multiple dyes per antibody can lead to dye self‐quenching. present work reports a methodology biotinylated zwitterionic dye‐loaded polymeric nanoparticles (NPs). A rationally designed hydrophobic polymer, poly(ethyl methacrylate) bearing charged, and biotin groups (PEMA‐ZI‐biotin), enables preparation small (14 nm) bright fluorescent NPs loaded large quantities...
At least one-third of patients with epithelial ovarian cancer (OC) present ascites at diagnosis and almost all have recurrence especially because the propensity OC cells to spread in abdominal cavity leading peritoneal metastasis. The influence on development pre-metastatic niches, biological mechanisms cell colonization mesothelium, remains poorly understood. Here, we show that weakens mesothelium by affecting morphology mesothelial destabilizing their distribution cycle. Ascites also...
Detection and imaging of RNA at the single-cell level is utmost importance for fundamental research clinical diagnostics. Current techniques analysis, including fluorescence in situ hybridization (FISH), are long, complex, expensive. Here, we report a methodology amplified FISH (AmpliFISH) that enables simpler faster using small ultrabright dye-loaded polymeric nanoparticles (NPs) functionalized with DNA. We found size NPs (below 20 nm) was essential their access to intracellular mRNA...
Abstract Nucleic acids are important biomarkers in cancer and viral diseases. However, their ultralow concentration biological/clinical samples makes direct target detection challenging, because it leads to slow hybridization kinetics with the probe its insufficient signal‐to‐noise ratio. Therefore, RNA is done by molecular (target) amplification, notably RT‐PCR, which a tedious multistep method that includes nucleic acid extraction reverse transcription. Here, based on ultrabright...
Nucleic-acid aptamers are of strong interest for diagnosis and therapy. Compared with antibodies, they smaller, stable upon variations in temperature, easy to modify, have higher tissue-penetration abilities. However, been little described as detection probes histology studies human tissue sections. In this study, we performed fluorescence imaging two targeting cell-surface receptors EGFR integrin α5β1, both involved the aggressiveness glioblastoma. The aptamers' cell-binding specificities...
Objectives To assess the prevalence of HIV-1 infection among non-intravenous drug user (IVDU) female prostitutes in Spain and to determine risk factors for this population. Design Cross-sectional seroepidemiological study 519 non-IVDU prostitutes. Setting: Four university hospitals. Methods All participants completed a questionnaire provided serum sample. Serum samples were tested antibodies against HIV-1, hepatitis C virus (HCV) Treponema pallidum. Results Twelve out (2.31%)...
EGFR (epidermal growth factor receptor), a member of the ErbB tyrosine kinase receptor family, is clinical therapeutic target in numerous solid tumours. overexpression glioblastoma (GBM) drives cell invasion and tumour progression. However, trials were disappointing, molecular basis to explain these poor results still missing. endocytosis membrane trafficking, which tightly regulate oncosignaling, are often dysregulated glioma. In previous work, we showed that inhibitors, such as gefitinib,...
ABSTRACT Active targeting is based on the binding of ligands to receptors present surface targeted cells, in order promote internalization drugs conjugated ligands. Several conjugates are already use or under development for active tumors, most widely known being antibody-drug (ADC). They combine specificity monoclonal antibodies with cytotoxicity chemotherapeutic molecules. Other than antibodies, nucleic-acid aptamers, promising deliver by tumor cells. The therapeutic efficacy largely...
Fluorescent dye-loaded polymeric nanoparticles have been extensively developed for cellular imaging as they are bright and versatile tools with large choice of polymers, dyes surface chemistry. Here, we report preparation fluorescent nanomaterials high aspect ratio – nanorods, study their uptake bioimaging applications. nanorods prepared by scission electrospun nanofibers using ultrasonication. To achieve brightness, loaded cationic (rhodamine or cyanines) bulky hydrophobic counterions that...
Negatively charged phospholipids can serve as biomarkers for a number of biological processes allowing detection apoptosis in animal cells, drug-induced phospholipidosis well bacteria. Selective thus represents an important but challenging goal. The development fluorescent probes capable selective recognition specific be promising solution to the problem. Along this line, we have developed macrocycle-dye-anchor conjugates, which macrocycle recognizes phosphate residue, hydrocarbon anchors...