A5012779924- Advanced biosensing and bioanalysis techniques
- Advanced Electron Microscopy Techniques and Applications
- Nuclear Structure and Function
- CRISPR and Genetic Engineering
Institut de génétique et de biologie moléculaire et cellulaire
2023
Institut de Biologie Intégrative de la Cellule
2023
Abstract Advances in cryo‐electron microscopy (EM) enable imaging of protein assemblies within mammalian cells a near native state when samples are preserved by cryogenic vitrification. To accompany this progress, specialized EM labelling protocols must be developed. Gold nanoparticles (AuNPs) 2 nm synthesized and functionalized to bind selected intracellular targets inside living human detected vitreous sections. As proof concept, thioaminobenzoate‐, thionitrobenzoate‐coordinated gold on...
Despite advances in Electron Microscopy (EM) that enable to image protein assemblies within vitreous sections of cells at nearly atomic resolution, labelling is still necessary locate small proteins or rare complexes. Gold immunolabelling has been used for decades localise specific cellular sections. However, current gold particle-antibody conjugates are not built with enough chemical precision match the resolution offered by cryo-EM methodology. Furthermore, as a close native specimen state...