A5048870109- Monoclonal and Polyclonal Antibodies Research
- Genomics and Chromatin Dynamics
- Glycosylation and Glycoproteins Research
- Advanced biosensing and bioanalysis techniques
- Nanocluster Synthesis and Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Nanoparticle-Based Drug Delivery
- Nanoplatforms for cancer theranostics
- Cancer-related Molecular Pathways
- Enzyme Structure and Function
- Chemical Synthesis and Analysis
- Advanced Biosensing Techniques and Applications
- DNA Repair Mechanisms
Institut de Cancérologie Strasbourg
2022
Université de Strasbourg
2018-2022
Biotechnologie et Signalisation Cellulaire
2019-2022
Centre National de la Recherche Scientifique
2018-2021
Phosphorylated histone H2AX (γ-H2AX), a central player in the DNA damage response (DDR), serves as biomarker of double-strand break repair. Although is generally visualized by formation γ-H2AX foci injured nuclei, it unclear whether widespread uniform nuclear (called pan-nuclear) pattern occurring upon intense replication stress (RS) linked to DDR. Using novel monoclonal antibody that binds exclusively phosphorylated C-terminus H2AX, we demonstrate phosphorylation systematically pan-nuclear...
Histone H2AX phosphorylated at serine 139 (γ-H2AX) is a hallmark of DNA damage, signaling the presence double-strand breaks and global replication stress in mammalian cells. While γ-H2AX can be visualized with antibodies fixed cells, its detection living cells was so far not possible. Here, we used immune libraries phage display to isolate nanobodies that specifically bind γ-H2AX. We solved crystal structure most soluble nanobody complex phosphopeptide corresponding C-terminus show atomic...
For biological and medical applications, a definable nanomaterial knowledge on its fate after administration are highly recommended if not mandatory. Here, we synthesized water-soluble gold nanocluster by sodium borohydride reduction of chloroauric acid in the presence 5,5′-dithio-bis(2-nitrobenzoic acid). The resulting displayed physical characteristics cluster containing an inner core about 100 atoms that was surrounded organic monolayer made 30 thioaminobenzoic acids (TAB) 14 anionic...
The homogeneous labeling of antibodies and their fragments is a critical step for the generation robust probes used in immuno-detection applications. To date, numerous chemical, genetic peptide-based site-specific coupling methods have been developed. Among these methods, co-assembling peptide-tags one most straightforward versatile solutions. Here, we describe nanobodies through use two co-associating peptides tags, E3 K3, originating from tetramerization domain p53. These K3-tags provide...