A5046720265- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Protein purification and stability
- RNA Interference and Gene Delivery
- Advanced Biosensing Techniques and Applications
- Genetic Neurodegenerative Diseases
- Receptor Mechanisms and Signaling
- Virus-based gene therapy research
- Cell Adhesion Molecules Research
- Signaling Pathways in Disease
- Chemical Synthesis and Analysis
- Mitochondrial Function and Pathology
- Transgenic Plants and Applications
- Immunotherapy and Immune Responses
- HIV Research and Treatment
- Advanced biosensing and bioanalysis techniques
- Immune Cell Function and Interaction
- Molecular Biology Techniques and Applications
- Antimicrobial Peptides and Activities
- Animal Virus Infections Studies
- Protein Hydrolysis and Bioactive Peptides
- Plant Virus Research Studies
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- T-cell and B-cell Immunology
École Supérieure de Biotechnologie de Strasbourg
2004-2024
Centre National de la Recherche Scientifique
2007-2024
Biotechnologie et Signalisation Cellulaire
2013-2024
Université de Strasbourg
2011-2023
École Normale Supérieure - PSL
2005-2013
Institut de génétique et de biologie moléculaire et cellulaire
2008
Laboratoire de Biologie Physico-Chimique des Protéines Membranaires
2006
Laboratoire des Biomolécules
2006
Institut de Biologie Moléculaire et Cellulaire
1993-2003
Institut de Biologie Moléculaire des Plantes
1995-1999
Arthritis in the K/BxN mouse model is provoked by pathogenic antibodies (Abs) directed against a ubiquitously expressed protein, glucose-6-phosphate isomerase (GPI). To begin dissecting repertoire of arthritogenic immunoglobulins (Igs) model, and to provide basis for comparison with RA patientswe have generated anti-GPI monoclonal Abs (mAbs) from spontaneously activated B cells lymphoid organs arthritic mice. cell clones specificities were present at extraordinarily high frequencies spleen,...
We have optimized the expression level of 20 mammalian G protein-coupled receptors (GPCRs) in methylotrophic yeast Pichia pastoris. found that altering parameters, including growth temperature, and supplementation culture medium with specific GPCR ligands, histidine, DMSO increased amount functional receptor, as assessed by ligand binding, more than eightfold over standard conditions. Unexpectedly, we overall proteins expressed, most cases, varied only marginally between Accordingly,...
Three analogues of the model peptide sequence IRGERA corresponding to COOH-terminal residues 130-135 histone H3 were synthesized, and their antigenicity, immunogenicity, resistance trypsin compared those natural L-peptide. The three correspond D-enantiomer, containing only D-residues, two retro-peptides NH-CO bonds instead bonds. chirality each residue was maintained in retro-peptide inverted retro-inverso-peptide. Antibodies four produced by injecting BALB/c mice with peptides covalently...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDetermination of kinetic constants for the interaction between a monoclonal antibody and peptides using surface plasmon resonanceDaniele Altschuh, Marie Christine Dubs, Etienne Weiss, Gabrielle Zeder-Lutz, Marc H. V. Van RegenmortelCite this: Biochemistry 1992, 31, 27, 6298–6304Publication Date (Print):July 1, 1992Publication History Published online1 May 2002Published inissue 1 July...
Abstract Cervical cancer is caused by high-risk types of human papillomaviruses (HPV) that encode the E6 and E7 oncogenes. Silencing gene expression in HPV-positive cell lines transfection small interfering RNA (siRNA) with cationic lipids restores dormant p53 tumor suppressor pathway. Because can also be used for intracytoplasmic delivery proteins, we tested whether monoclonal antibodies bind to HPV16 neutralize its biological activity vitro could restore function cells. Here, show 4C6...
ABSTRACT To acquire iron, Pseudomonas aeruginosa secretes the fluorescent siderophore pyoverdine (Pvd), which chelates iron and shuttles it into cells via specific outer membrane transporter FpvA. We studied role of other metals in binding transport Pvd by FpvA conclude that there is no significant affinity between metal-free Pvd. found vivo complex iron-free FpvA-Pvd fact a with aluminum (FpvA-Pvd-Al) formed from trace growth medium. When was cultured medium had been treated metal resin,...
A long-standing pathomechanistic model proposes that the polyglutamine (polyQ)-length-dependent toxicity threshold observed in all polyQ diseases is triggered by a conformational change within monomer occurs only above certain length. If true, this yet undefined and elusive mutant-specific toxic conformation would constitute direct therapeutic target. Three anti-polyQ antibodies—MW1, 1C2 3B5H10—have been extensively used to probe of polyQ. The crystal structure MW1 epitope reveals linear,...
CD28 and CTLA-4 are the major costimulatory receptors on naive T cells. But it is not clear why monovalent whereas bivalent for their shared ligands CD80/86. We generated constructs by fusing extracellular domains of or CD80 with intracellular CD28. Bivalent were ligated recombinant without TCR coligation. Monovalent ligation did induce responses unless was coligated. By contrast, induced in absence engagement. To extend these findings to primary cells, we used novel superagonistic...
Histone H2AX phosphorylated at serine 139 (γ-H2AX) is a hallmark of DNA damage, signaling the presence double-strand breaks and global replication stress in mammalian cells. While γ-H2AX can be visualized with antibodies fixed cells, its detection living cells was so far not possible. Here, we used immune libraries phage display to isolate nanobodies that specifically bind γ-H2AX. We solved crystal structure most soluble nanobody complex phosphopeptide corresponding C-terminus show atomic...
Huntington's disease (HD) is caused by the expansion mutation above a length threshold of polyglutamine (polyQ) stretch in huntingtin (Htt) protein. Mutant Htt (mHtt) pathogenicity proposed to rely on its malfunction and propensity misfold aggregate. has scaffolding properties been reported interact with hundreds partners. Many interactors show apparent increased or decreased affinity (dysinteraction) for mHtt, which may account selective malfunctions striatal degeneration HD. These...
Pyoverdine-mediated iron uptake by the FpvA receptor in outer membrane of Pseudomonas aeruginosa is dependent on inner protein TonB1. This energy transducer couples proton-electrochemical potential to transport event. To shed more light upon this process, a recombinant TonB1 lacking N-terminal anchor (TonB(pp)) was constructed. was, after expression Escherichia coli, purified from soluble fraction lysed cells means an hexahistidine or glutathione S-transferase (GST) tag. Purified...
Whereas the concentration of a biomolecule simply refers to amount chemical substance per unit volume, its active relational parameter that has meaning only with respect molecule's ability interact specifically one particular ligand. When proteins are studied in biological context, it is biologically relevant, and not total correctly incorrectly folded molecules. Using biosensor instrument biomolecules preparation can be measured by injecting at different flow rates onto sensor chip surface...
In the continuous exploration of VHH chemistry, biochemistry and therapeutic future use, we investigated two different production strategies this small antibody-like protein, using an anti-HER2 as a model. The total chemical synthesis 125 amino-acid peptide was performed with reasonable yield, even if optimization will be necessary to upgrade kind production. parallel, expressed same sequence in hosts: Escherichia coli Pichia pastoris. Both productions were successful led fair amount VHHs....
Abstract Cyclic peptides containing redox‐stable thioether bridges might provide a useful alternative to disulfide‐bridged bioactive peptides. We report the effect of replacing disulfide bridge with lanthionine linkage in 16‐mer cyclic peptide that binds death receptor 5 (DR5, TRAIL‐R2). Upon covalent oligomerisation, has previously shown similar behaviour TNF‐related apoptosis inducing ligand (TRAIL), by selectively triggering DR5 cell pathway. The structural and biological properties...
Different conformers of the immunosuppressant cyclosporin A have been observed in structural studies isolated molecule and its complex with cyclophilin or an Fab fragment. The factors that control this conformational change are not well understood. Variations amount formed antibody were measured as a function time after adding to proteins, using Pharmacia BIAcore biosensor instrument. Up 1 hour was needed reach maximum formation solution, which is likely reflect for transition cyclosporin....
The ectopic expression of antibody fragments inside mammalian cells (intrabodies) is a challenging approach for probing and modulating target activities. We previously described the shuttling activity intracellularly expressed Escherichia coli β‐galactosidase conferred by single‐chain Fv (scFv) fragment 13R4 equipped with nuclear import/export signals. Here, appending to scFvs proteolytic PEST signal sequence (a protein region rich in proline, glutamic acid, serine threonine) mouse ornithine...