A5085438624- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Protein Structure and Dynamics
- Hepatitis C virus research
- Ultrasound and Hyperthermia Applications
- Advanced NMR Techniques and Applications
- Chemical Synthesis and Analysis
- HIV Research and Treatment
- Molecular spectroscopy and chirality
- Protein purification and stability
- Protein Degradation and Inhibitors
- Bacterial biofilms and quorum sensing
- NMR spectroscopy and applications
- DNA and Nucleic Acid Chemistry
- Hepatitis B Virus Studies
- Ubiquitin and proteasome pathways
- Enzyme Structure and Function
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Biochemical and Structural Characterization
- Antimicrobial Resistance in Staphylococcus
- Ultrasound Imaging and Elastography
- HIV/AIDS drug development and treatment
- Photoacoustic and Ultrasonic Imaging
- Protein Tyrosine Phosphatases
University of Rome Tor Vergata
2010-2023
European Brain Research Institute
2013-2014
IRBM Science Park
1996-2013
Chiesi (France)
2012-2013
Institute of Molecular Biology and Pathology
1994-2009
MSD (Italy)
2008
National Institutes of Health
1991-2007
University of Campania "Luigi Vanvitelli"
2007
Institut Pasteur
1995
National Institute of Diabetes and Digestive and Kidney Diseases
1992
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBackbone dynamics of calmodulin studied by nitrogen-15 relaxation using inverse detected two-dimensional NMR spectroscopy: the central helix is flexibleGaetano Barbato, Mitsuhiko Ikura, Lewis E. Kay, Richard W. Pastor, and Ad BaxCite this: Biochemistry 1992, 31, 23, 5269–5278Publication Date (Print):July 16, 1992Publication History Published online1 May 2002Published inissue 16 July...
Heteronuclear 2D and 3D NMR experiments were carried out on recombinant Drosophila calmodulin (CaM), a protein of 148 residues with molecular mass 16.7 kDa, that is uniformly labeled 15N 13C to level greater than 95%. Nearly complete 1H side-chain assignments for all amino acid are obtained by using the HCCH-COSY HCCH-TOCSY rely large heteronuclear one-bond scalar couplings transfer magnetization establish through-bond connectivities. The secondary structure this in solution has been...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNMR Analysis of Molecular Flexibility in Solution: A New Method for the Study Complex Distributions Rapidly Exchanging Conformations. Application to a 13-Residue Peptide with an 8-Residue LoopD. O. Cicero, G. Barbato, and R. BazzoCite this: J. Am. Chem. Soc. 1995, 117, 3, 1027–1033Publication Date (Print):January 1, 1995Publication History Published online1 May 2002Published inissue 1 January...
HIV-1 entry into cells is mediated by the envelope glycoprotein receptor-binding (gp120) and membrane fusion-promoting (gp41) subunits. The gp41 heptad repeat 1 (HR1) domain molecular target of fusion-inhibitor drug enfuvirtide (T20). HR1 sequence highly conserved therefore considered an attractive for vaccine development, but it unknown whether antibodies can access HR1. Herein, we use gp41-based peptides to select a human antibody, 5H/I1-BMV-D5 (D5), that binds inhibits assembly fusion...
The purpose of this study was to evaluate the antimicrobial efficacy five different proteases belonging two families on Staphylococcus aureus and epidermidis strains.We used three serine metalloproteases in single species biofilm formation assays human cell invasion processes. Following each protease incubation with bacterial cells, surface protein patterns were analysed by SDS-PAGE zymography. Some differently expressed proteins identified mass spectrometry.The effect tested not related...
The HECT-containing E3 ubiquitin ligase Itch mediates the degradation of several proteins, including p63 and p73, involved in cell specification fate. contains four WW domains, which are essential for recognition on target substrate, a short proline-rich sequence. Several signaling complexes containing these domains have been associated with human diseases such as muscular dystrophy, Alzheimer's or Huntington's diseases. To gain further insight into structural determinants Itch-WW2 domain,...
The NS3 region of the hepatitis C virus encodes for a serine protease activity, which is necessary processing nonstructural viral polyprotein. minimal domain with proteolytic activity resides in N terminus, where structural tetradentate zinc binding site located. ligands being been identified by x-ray crystallography as three cysteines (Cys97, Cys99, and Cys145) one histidine residue (His149), postulated to coordinate metal through water molecule. In this article, we present an analysis role...
The E3 ubiquitin ligase Itch mediates the degradation of p63 protein. contains four WW domains which are pivotal for substrate recognition process. Indeed, this domain is implicated in several signalling complexes crucially involved human diseases including Muscular Dystrophy, Alzheimer's Disease, and Huntington Disease. highly compact protein-protein binding modules that interact with short proline-rich sequences. present belong to Group I type, binds polypeptides a PY motif characterized...
The molecular determinants necessary and sufficient for recognition of its specific DNA target are contained in the C-terminal domain (H-NSctd) nucleoid-associated protein H-NS. H-NSctd protects from DNaseI cleavage a few short segments H-NS-sensitive hns promoter whose sequences closely match recently identified H-NS consensus motif (tCG(t/a)T(a/t)AATT) and, alone or fused to oligomerization phage lambda CI repressor, inhibits transcription vitro vivo. importance is indicated by fact that...
Neuromedin U (NMU) is an endogenous peptide implicated in the regulation of feeding, energy homeostasis, and glycemic control, which being considered for therapy obesity diabetes. A key liability NMU as a therapeutic its very short half-life vivo. We show here that conjugation to human serum albumin (HSA) yields compound with long circulatory half-life, maintains full potency at both peripheral central receptors. Initial attempts conjugate via prevalent strategy reacting maleimide derivative...
A procedure is described that affords complete 1H, 13C and 15N resonance assignment in proteins of up to about 25 kDa. The new approach requires uniform isotopic enrichment the protein with correlates resonances adjacent nuclei using relatively large well-resolved one-bond J couplings. Spectral overlap, a common problem application two-dimensional NMR, removed by increasing dimensionality methods three or four, without number observed resonances. With assignments available, nuclear...