A5065744840- Monoclonal and Polyclonal Antibodies Research
- Hepatitis B Virus Studies
- Glycosylation and Glycoproteins Research
- Angiogenesis and VEGF in Cancer
- Bacteriophages and microbial interactions
- Immunotherapy and Immune Responses
- Peptidase Inhibition and Analysis
- Galectins and Cancer Biology
- Transgenic Plants and Applications
- Protein Degradation and Inhibitors
- Genetic and phenotypic traits in livestock
- Plant Virus Research Studies
- Protease and Inhibitor Mechanisms
- Animal Virus Infections Studies
- Cancer Research and Treatments
- Cancer Immunotherapy and Biomarkers
- Genetic diversity and population structure
- Click Chemistry and Applications
- Viral gastroenteritis research and epidemiology
- Colorectal Cancer Treatments and Studies
- Vibrio bacteria research studies
- Biotechnology and Related Fields
- Cancer Treatment and Pharmacology
- Advanced Biosensing Techniques and Applications
- Inflammatory mediators and NSAID effects
Istanbul Aydın University
2019-2023
Scientific and Technological Research Council of Turkey
2000-2017
TÜBİTAK Marmara Research Center
2011-2017
Biotechnology Institute
2011-2014
Abstract Human vascular endothelial growth factor (VEGF) and its receptor (VEGFR‐2/kinase domain [KDR]) play a crucial role in angiogenesis, which makes the VEGFR‐2 signaling pathway major target for therapeutic applications. In this study, single‐chain antibody phage display library was constructed from spleen cells of mice immunized with recombinant human soluble extracellular VEGFR‐2/KDR consisting all seven domains (sKDR D1–7) to obtain antibodies that block VEGF binding VEGFR‐2. Two...
Summary The horse has been a food source, but more importantly, it means for transport. Its domestication was one of the crucial steps in history human civilization. Despite archaeological and molecular studies carried out on domestication, which would contribute to conservation breeds, details horses still remain be resolved. We employed 21 microsatellite loci mitochondrial control region partial sequences analyse genetic variability within among four Anatolian native Ayvacık Pony, Malakan...
Hepatitis B virus (HBV) is one of the major causes chronic hepatitis, cirrhosis and liver cancer. In combating HBV infections, diagnosis vaccination are therefore critical. The hepatitis surface antigen (HBsAg) a key target molecule in developing vaccines diagnostic systems. To date, although HBsAg has been expressed bacteria, yeasts mammalian cells, there still limitations existing ones, which leave necessity for searching new production methods. this study, simple phage display-based...
The objective of this study was to obtain transgenic tobacco (Nicotiana tabacum L. cv. Samsun NN) plants resistant mosaic virus, TMV-vulgare strain, by the expression a single-chain variable fragment (scFv-anti-TMV) against TMV coat protein. scFv-anti-TMV gene made combining coding sequences heavy and light chain domains full antibody molecule with sequence encoding flexible linker. cloned under control 35S promoter in plant vector pBI121, used for Agrobcterium-mediated transformation...
Hepatitis B virus (HBV) infection is a worldwide health problem. More than 400 million people are chronic HBV carriers in the world. Infected individuals at high risk of developing liver cirrhosis and hepatocellular carcinoma as main consequences HBV. The discoveries fast diagnostic systems new therapeutic applications crucial fight against viral hepatitis. In this paper we present generation single-chain variable fragment (scFv) from mouse monoclonal antibody specific to surface antigen...
Development of monoclonal antibody therapeutics against vascular endothelial growth factor receptor 2 (VEGFR-2) protein, which is the main regulator in angiogenesis, has been a major challenge for years. In current study, we engineer an inclusion body forming single-chain variable fragment (scFv) VEGFR-2 by using complementarity determining regions (CDR) grafting technique to improve its solubility and investigate activity engineered molecule. CDR sequences target scFv were grafted into...
Potato virus Y (PVY) is distributed around the world and causes an important economic loss. We report here, development of a recombinant antibody in form single chain variable fragment (scFv) against PVY by using phage display technology. A library fragments was constructed region (V) genes B cells derived from spleens immunized Balb/CJ mice. The selection scFv with ability to bind performed biopanning tested phage-enzyme linked immunosorbent assay (ELISA). ELISA tests showed that scFvs...
Fajlar organizmada humoral ve hücresel immün yanıtı uyandırabilen yüksek immünojenik özelliğe sahip moleküllerdir potansiyel aşı taşıyıcısı olarak kullanılmaktadırlar. Bu çalışmada Hepatit B yüzey antijeni (HBsAg) tam molekül M13 faj yüzeyinde füzyon protein ekprese edilmiş rekombinant fajın antijenik etkisi BALB/cJ farelerinde incelenmiştir. Farelerin dozda (10 12 ) ile immünize edilmesinin normal faja kıyasla daha antikor yanıtına neden olduğu gösterilmiştir. elde edilen olumlu yanıt...
The following topics are dealt with: medical image processing; biomechanics; diseases; cellular biophysics; neurophysiology; signal cancer; brain; biomedical MRI; prosthetics.
Peptide therapeutics are gaining increasing attention in healthcare because of their many advantages over traditional small-molecule drugs.Peptides have such as being naturally present the body, specific at a level that drugs cannot reach, and half-life body.These make peptide promising area research for developing new treatments.According to report on global Therapeutics market, with growth rate 6.6%, industry is expected reach $69.3 billion by 2030.As this continues advance, will potential...