A5026890553- RNA Interference and Gene Delivery
- Polymer Surface Interaction Studies
- Immunotherapy and Immune Responses
- Advanced biosensing and bioanalysis techniques
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- Antimicrobial Peptides and Activities
- Nanoparticle-Based Drug Delivery
- Virus-based gene therapy research
- Click Chemistry and Applications
- Immune Cell Function and Interaction
- Bone Tissue Engineering Materials
- Advanced Drug Delivery Systems
- Molecular Junctions and Nanostructures
- Lipid Membrane Structure and Behavior
- T-cell and B-cell Immunology
- Chemical Synthesis and Analysis
- Glycosylation and Glycoproteins Research
- Hydrogels: synthesis, properties, applications
- Dendrimers and Hyperbranched Polymers
- Photochromic and Fluorescence Chemistry
- biodegradable polymer synthesis and properties
- Electrospun Nanofibers in Biomedical Applications
- Inflammasome and immune disorders
- vaccines and immunoinformatics approaches
Centre National de la Recherche Scientifique
2015-2025
Inserm
2002-2025
Université de Strasbourg
2015-2024
Laboratoire de Chémo-biologie synthétique et thérapeutique
2014-2024
Université Libre de Bruxelles
2022
Fondation Jean-Marie Lehn
2011-2018
Laboratoire de Biophotonique et Pharmacologie
2018
Pharmac
2017
Centre National pour la Recherche Scientifique et Technique (CNRST)
2012
Genethon (France)
2010
Secondary bacterial infections often complicate viral respiratory infections. We hypothesize that perturbation of the gut microbiota during influenza A virus (IAV) infection might favor superinfection. Sublethal with transiently alters composition and fermentative activity in mice. These changes are attributed part to reduced food consumption. Fecal transfer experiments demonstrate IAV-conditioned compromises lung defenses against pneumococcal infection. In mechanistic terms, production...
Mechanical properties of model and natural gels have recently been demonstrated to play an important role in various cellular processes such as adhesion, proliferation, differentiation, besides events triggered by chemical ligands. Understanding the biomaterial/cell interface is particularly many tissue engineering applications implant surgery. One final goals would be control precisely at biomaterial surface guide regeneration. In this work, we investigate substrate mechanical effect on...
Abstract Polyelectrolyte multilayers (PEMs) are now widely used for biomedical applications. In this work, we investigated the primary osteoblast adhesion properties of PEMs poly( L ‐lysine) (PLL), ‐glutamic acid) (PGA), poly(alginic (Palg), and poly(galacturonic (Pgal). order to compensate poor as‐synthesized films, two kinds film modifications were achieved: a purely physical modification by crosslinking, chemical grafting arginine–glycine–aspartic acid (RGD) peptide PGA. Crosslinking was...
The inherent room temperature mending and self-healing properties of saloplastic PAA/PAH CoPECs are studied. After ultracentrifugation polyelectrolyte complexes, tough, elastic materials obtained that undergo facilitated by salt. At intermediate salt concentrations the remain enough to recover their original shape while chains mobile repair cut, thus leading actual behavior. As a service our authors readers, this journal provides supporting information supplied authors. Such peer reviewed...
Objective To evaluate whether miR-20a belonging to the cluster miR-17–92 is a negative regulator of inflammation in rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS) by modulating expression apoptosis signal-regulating kinase (ASK) 1, key component toll-like receptors 4 pathway, upstream p38 mitogen-activated protein kinase. Methods Evaluation and ASK1 mRNA was performed RT-qPCR. assessed western blotting. Overexpression transfection RA FLS THP-1 cells with mimics. Interleukin...
The mRNA vaccine technology has promising applications to fight infectious diseases as demonstrated by the licensing of two mRNA-based vaccines, Comirnaty® (Pfizer/BioNtech) and Spikevax® (Moderna), in context Covid-19 crisis. Safe effective delivery systems are essential performance these vaccines lipid nanoparticles (LNPs) able entrap, protect deliver vivo considered many current "best class". Nevertheless, mRNA/LNP still some limitations, one them being thermostability, evidenced...
The buildup and secondary structure of poly(l-glutamic acid)/poly(allylamine) (PGA/PAH) multilayer films were investigated by means optical waveguide lightmode spectroscopy, quartz crystal microbalance, Fourier transform infrared spectroscopy in attenuated total reflection mode. thickness the mass these grow exponentially with number deposited bilayers. Moreover, PGA undergoes a random/α-helix transition when interacting PAH during film process. This structural leads to (PGA/PAH)i an α-helix...
An efficient and convenient chemoselective conjugation method based on "click chemistry" was developed for coupling ligands to the surface of preformed liposomes. It can be performed under mild conditions in aqueous buffers; use a water soluble Cu(I) chelator, such as bathophenanthrolinedisulfonate, essential obtain good yields reasonable reaction times. A model achieved which, single step, an unprotected alpha-D-mannosyl derivative carrying spacer arm functionalized with azide group...
Cross-linked polyelectrolyte multilayer films (CL PEM) have an increased rigidity and are mechanically more resistant than native (e.g., uncrosslinked) films. However, they still biodegradable, which make them interesting candidates for biomedical applications. In this study, CL PEM been explored their multifunctional properties as (i) resistant, (ii) (iii) bioactive Toward end, we investigated drug loading into chitosan/hyaluronan (CHI/HA) poly(l-lysine)/hyaluronan (PLL/HA) by simple...
A new antibacterial coating made of poly(l-lysine)/hyaluronic acid (PLL/HA) multilayer films and liposome aggregates loaded with silver ions was designed. Liposomes filled an AgNO3 solution were first aggregated by the addition PLL in solution. The obtained micrometer-sized then deposited on a PLL/HA film, playing role spacer support. Finally, HA/PLL/HA capping layers top architecture to form composite coating. Release encapsulated from this followed triggered upon temperature increase over...
Immunotherapy aiming at enhancing innate and acquired host immunity is a promising approach for cancer treatment. The invariant NKT (iNKT) cell ligand α-galactosylceramide (α-GalCer) holds great promise in therapy, although several concerns limit its use clinics, including the uncontrolled response it promotes when delivered nonvectorized form. Therefore, development of delivery systems to vivo target immune cells might be valuable option optimize iNKT cell-based antitumor responses. Using...
The comparative reactivity of maleimide and bromoacetyl groups with thiols (2-mercaptoethanol, free cysteine, cysteine residues present at the N-terminus peptides) was investigated in aqueous media. These studies were performed (i) water-soluble functionalized model molecules, i.e., polyoxyethylene-based spacer arms that could also be coupled to lipophilic anchors destined incorporated into liposomes, (ii) small unilamellar liposomes carrying their surface these thiol-reactive functions. Our...
An important challenge for the development of new generations vaccines is efficient delivery antigens to antigen presenting cells such as dendritic cells. In present study we compare interaction plain and targeted liposomes, containing mono-, di-, tetraantennary mannosyl lipid derivatives, with human monocyte-derived immature (iDCs). Whereas mannose receptor-mediated endocytosis by iDCs was observed mannosylated in contrast, only nonspecific little uptake liposomes. accordance clustering...
We report the covalent layer-by-layer construction of polyelectrolyte multilayer (PEM) films by using an efficient electrochemically triggered Sharpless click reaction. The reaction is catalyzed Cu(I) which generated in situ from Cu(II) (originating dissolution CuSO4) at electrode constituting substrate film. film buildup can be controlled application a mild potential inducing reduction to absence any reducing agent or ligand. experiments were carried out electrochemical quartz crystal...
Abstract Durable compact polyelectrolyte complexes (CoPECs) with controlled porosity and mechanical properties are prepared by ultracentrifugation. Because the starting materials, poly(allylamine hydrochloride) (PAH) poly(acrylic acid sodium salt) (PAA), weak acids/bases, both composition morphology solution pH. In addition, nonequilibrium nature of complexation can be exploited to provide a range compositions porosities under influence addition order speed, concentration. Confocal...
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