A5086665067- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Immunotherapy and Immune Responses
- Gout, Hyperuricemia, Uric Acid
- Inflammasome and immune disorders
- Sepsis Diagnosis and Treatment
- RNA modifications and cancer
- Immune Response and Inflammation
- Inflammation biomarkers and pathways
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Cancer Treatment and Pharmacology
- Proteoglycans and glycosaminoglycans research
- Immune cells in cancer
- Vasculitis and related conditions
- Phytochemistry and Bioactive Compounds
- Cellular Mechanics and Interactions
- Chemokine receptors and signaling
- Veterinary medicine and infectious diseases
- Cancer-related molecular mechanisms research
- Viral Infections and Vectors
- Antimicrobial Resistance in Staphylococcus
- Neonatal and Maternal Infections
Inserm
2014-2023
Université Grenoble Alpes
2017-2023
Centre National de la Recherche Scientifique
2017-2023
Toxicologie, Pharmacologie et Signalisation Cellulaire
2018-2023
Hôpital Civil, Strasbourg
2021-2022
Université de Strasbourg
2019-2021
Immuno-Rhumathologie moléculaire
2020-2021
Institut pour l'avancée des biosciences
2014-2020
Institut de Biosciences et Biotechnologies
2016
Université Joseph Fourier
2015
Metastasis is a major cause of death in cancer patients. The extracellular matrix molecule tenascin-C known promoter metastasis, however the underlying mechanisms are not well understood. To further analyze impact on progression we generated MMTV-NeuNT mice that develop spontaneous mammary tumors, knockout background. We also developed syngeneic orthotopic model which tumor cells derived from tumor. Tumor were transfected with control shRNA or to knockdown expression and, grafted into gland...
à la diffusion de documents scientifiques niveau recherche, publiés ou non, émanant des établissements d'enseignement et recherche français étrangers, laboratoires publics privés.
Abstract The splice variant sVEGFR1-i13 is a truncated version of the cell membrane-spanning VEGFR1 receptor that devoid its transmembrane and tyrosine kinase domains. We recently showed contribution to progression response squamous lung carcinoma anti-angiogenic therapies. In this study, we identify VEGF165, VEGF-A, as regulator expression in these tumors, further show VEGF 165 cooperates with transcription factor SOX2 splicing SRSF2 control expression. also demonstrate therapies...
The extracellular matrix (ECM) molecule Tenascin-C (TNC) is well-known to promote tumor progression by multiple mechanisms. However, reliable TNC detection in tissues of banks remains limited. Therefore, we generated dromedary single-domain nanobodies Nb3 and Nb4 highly specific for human (hTNC) characterized the interaction with several approaches including ELISA, western blot, isothermal fluorescence titration negative electron microscopic imaging. Our results revealed binding both...
Tenascin C (TNC) is an extracellular matrix glycoprotein that recently emerged as immunomodulator. TNC-deficient (TNC-/-) mice were reported to have a reduced inflammatory response upon systemic administration of lipopolysaccharide, the toxic component gram-negative bacteria. Here, we investigated role TNC during pneumonia derived sepsis. TNC+/+ and TNC-/- infected with Klebsiella pneumoniae via airways sacrificed 24 42 h thereafter for further analysis. Pulmonary protein levels elevated...
While lung adenocarcinoma patients can somewhat benefit from anti-angiogenic therapies, with squamous cell carcinoma (SQLC) cannot. The reasons for this discrepancy remain largely unknown. Soluble VEGF receptor-1, namely sVEGFR1-i13, is a truncated splice variant of the membrane-spanning VEGFR1 that has no transmembrane or tyrosine kinase domain. sVEGFR1-i13 mainly viewed as an factor which counteracts VEGF-A/VEGFR signalling in endothelial cells. However, its role tumour cells poorly...
Tenascin C (TNC) is an extracellular matrix protein with immunomodulatory properties that plays a major role during tissue injury and repair. TNC levels are increased in patients pneumonia pneumosepsis, they associated worse outcomes. Methicillin-resistant Staphylococcus aureus (MRSA) Gram-positive bacterium causative pathogen nosocomial rising cause of community-acquired pneumonia. To study the MRSA-induced pneumonia, sufficient (TNC+/+) TNC-deficient (TNC-/-) mice were infected MRSA via...
Anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitides (AAV) are severe inflammatory disorders that often involve focal necrotizing glomerulonephritis (FNGN) and consequent glomerular scarring, interstitial fibrosis, chronic kidney disease. Robust murine models of scarring in FNGN may help to further our understanding deleterious processes still lacking. Here, we present a model based on combined administration antibodies against the basement membrane (GBM) myeloperoxidase...
Septic shock and disseminated intravascular coagulation (DIC) are known to be characterized by an endothelial cell dysfunction. The molecular mechanisms underlying this relationship are, however, poorly understood. In work, we aimed investigate human circulating IFN-α in patients with septic shock-induced DIC tested the potential role of Stat1 (signal transducer activator transcription 1) as a therapeutic target mouse model sepsis. For this, type I, II, III IFNs procoagulant microvesicles...
Radiotherapy, the most frequent treatment of oral squamous cell carcinomas (OSCC) besides surgery is employed to kill tumor cells but, radiotherapy may also promote relapse where immune-suppressive microenvironment (TME) could be instrumental. We established a novel syngeneic grafting model from carcinogen-induced tongue tumor, OSCC13, address impact on OSCC. This revealed similarities with human OSCC, recapitulating mutations found in smoking associated tumors, abundant infiltrating...
The role of Monosodium Urate (MSU) crystals in gout pathophysiology is well described, as the major impact IL-1b inflammatory reaction that constitutes hallmark disease. However, despite discovery NLRP3 inflammasome and its a Pattern Recognition Receptor linking detection danger signal to IL-1b; secretion vitro, precise mechanisms leading joint inflammation patients are still poorly understood. Here, we provide an extensive clinical, biological molecular characterization acute uratic mouse...