A5011347135- Venomous Animal Envenomation and Studies
- Monoclonal and Polyclonal Antibodies Research
- Toxin Mechanisms and Immunotoxins
- Aquaculture disease management and microbiota
- Ion channel regulation and function
- Biochemical and Structural Characterization
- Microbial Metabolism and Applications
- Probiotics and Fermented Foods
- Genomics and Phylogenetic Studies
- Advanced biosensing and bioanalysis techniques
- Insect and Pesticide Research
- Cell Adhesion Molecules Research
- Glycosylation and Glycoproteins Research
- Protein purification and stability
- Force Microscopy Techniques and Applications
- Antimicrobial Peptides and Activities
- Genetic factors in colorectal cancer
- Biosimilars and Bioanalytical Methods
- Mosquito-borne diseases and control
- Bacteriophages and microbial interactions
- Insect Resistance and Genetics
- Invertebrate Immune Response Mechanisms
- Identification and Quantification in Food
- Cardiac electrophysiology and arrhythmias
- Psoriasis: Treatment and Pathogenesis
Institut Pasteur de Tunis
2016-2025
Tunis El Manar University
2016-2025
Tunis University
2016-2025
Faculté de médecine de Tunis
2009-2024
Institut Pasteur
1996-2022
Laboratoire des Biomolécules
2021
Envenoming following scorpion sting is a common emergency in many parts of the world. Our aim was to ameliorate current 100-kDa horse plasma antivenom serum (PAS)-derived Fab'(2) more quickly reach highly diffusible toxins (7 kDa). We immunized dromedaries with from Androctonus australis hector (Aah) scorpions and cloned single-domain antibody fragments or nanobodies (15 kDa) their B cells. Nanobodies against AahI' toxin (with AahII most toxic compound venom) were retrieved libraries,...
Abstract The protein purity is generally checked using SDS‐PAGE, where densitometry could be used to quantify the bands. In literature, few studies have been reported image analysis for quantification of in SDS‐PAGE: that is, imaged with Stain‐Free™ technology. This study presents a protocol electrophoresis gels allows unknown proteins molecular weight markers as standards. Escherichia coli WK6/pHEN6 encoding bispecific nanobody CH10‐12 engineered by Pasteur Institute Tunisia was cultured...
Scorpion venom, containing highly toxic, small polypeptides that diffuse rapidly within the patient, causes serious medical problems. Nanobodies, single-domain antigen-binding fragments derived from dromedary heavy-chain antibodies, have a size closely matches of scorpion toxins. Therefore these nanobodies might be developed into potent immunotherapeutics to treat envenoming. Multiple sub-nanomolar affinity AahII, most toxic polypeptide Androctonus australis hector were isolated immunized...
Scorpion stings and snake bites are major health hazards that lead to suffering of victims high mortality. Thousands injuries associated with such venomous animals occur every year worldwide. In North Africa, more than 100,000 scorpion reported annually. An appropriate determination the 50% lethal doses (LD50) venoms appears be an important step assess (and compare) venom toxic activity. Such LD50 values also commonly used evaluate neutralizing capacity specific anti-venom batches. present...
Novel lactic acid bacteria isolated from different organs of freshwater fish were examined for their potential application as probiotics in raw and processed foods. Four isolates Enterococcus faecium Leuconostoc mesenteroides identified at the molecular level by 16S rRNA sequencing random amplification polymorphic DNA – polymerase chain reaction, antimicrobial activity against a panel pathogens food-poisoning was investigated. The whole bacteriocins 4 characterized enterobacterial repetitive...
Rapid and specific detection of pathogenic bacteria in fecal samples is critical importance for the diagnosis neonatal diarrhea veterinary clinics. Nanobodies are a promising tool treatment infectious diseases due to their unique recognition properties. In this study, we report design nanobody-based magnetofluorescent immunoassay sensitive Escherichia coli F17-positive strains (E. F17). For this, camel was immunized with purified F17A protein from F17 fimbriae nanobody library constructed by...
Insofar as they play an important role in the pathogenesis of colorectal cancer (CRC), this study analyzes serum profile cytokines, chemokines, growth factors, and soluble receptors patients with CRC cancer-free controls possible signatures. Serum levels 65 analytes were measured age- sex-matched using ProcartaPlex Human Immune Monitoring 65-Plex Panel. Of tested analytes, 8 cytokines (CSF-3, IFN-γ, IL-12p70, IL-18, IL-20, MIF, TNF-α TSLP), chemokines (fractalkine, MIP-1β, BLC, Eotaxin-1,...
During scorpion envenoming, highly toxic small polypeptides of the venom diffuse rapidly within victim, causing serious medical problems. Nanobodies (Nbs), recombinant single-domain antigen-binding fragments camel-specific heavy-chain only antibodies, offer special advantages in therapy over classic antibody due to their robustness and smaller size, matching size toxins. Recently, a potent AahII toxin-neutralizing Nb was identified. However, this NbAahII10 contains single Cys its first loop,...
Snakebite envenoming (SBE) remains a severely neglected public health issue, particularly affecting tropical and subtropical regions, with Africa experiencing an estimated 435,000 to 580,000 snakebites annually, leading high morbidity mortality rates, especially across Asia. Recognized as Neglected Tropical Disease, SBE management is further complicated by the inadequate efficacy of current antivenom treatments. Of particular concern are cobras (Naja sp.), whose neurotoxins can induce rapid...
Abstract Most large‐scale microbial production of recombinant proteins are based on Escherichia coli , yeasts, or filamentous fungi systems. Using eukaryotic hosts, antibody fragments generally expressed by targeting to the secretory pathway. This enables not only efficient disulfide bond formation but also secretion soluble and correctly folded product. For this goal, a vector was constructed produce single‐domain (NbAahI′22) directed against Aah I′ scorpion toxin using methylotrophic yeast...