A5088383728- Cell Adhesion Molecules Research
- Immunotherapy and Immune Responses
- RNA Research and Splicing
- Immune Cell Function and Interaction
- RNA modifications and cancer
- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- Chemokine receptors and signaling
- Immune cells in cancer
- Extracellular vesicles in disease
- Phagocytosis and Immune Regulation
- Computational Drug Discovery Methods
- Systemic Lupus Erythematosus Research
- Gout, Hyperuricemia, Uric Acid
- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- HIV Research and Treatment
- Platelet Disorders and Treatments
- Diabetes and associated disorders
- Reproductive System and Pregnancy
- Virology and Viral Diseases
- Immunodeficiency and Autoimmune Disorders
- interferon and immune responses
- T-cell and B-cell Immunology
- Inflammasome and immune disorders
Université de Strasbourg
2016-2025
Inserm
2016-2025
Fédération Hospitalo-Universitaire, Paris Center for Microbiome Medicine
2015-2025
Hôpitaux Universitaires de Strasbourg
2023
Urbana University
1959-2022
Communities In Schools of Orange County
2021-2022
Concord Consortium
2022
Immuno-Rhumathologie moléculaire
2017-2021
Bioinformatics Institute
2011
Institut de Biologie Intégrative de la Cellule
2011
Abstract Despite abundant evidence demonstrating that platelets foster metastasis, anti-platelet agents have low therapeutic potential due to the risk of hemorrhages. In addition, whether can regulate metastasis at late stages disease remains unknown. this study, we subject syngeneic models various thrombocytopenic regimes show provide a biphasic contribution metastasis. While potent intravascular binding tumor cells efficiently promotes further support outgrowth established metastases via...
Abstract Background MicroRNAs (miRNAs) are endogenous 21 to 23-nucleotide RNA molecules that regulate protein-coding gene expression in plants and animals via the interference pathway. Hundreds of them have been identified last five years very recent works indicate their total number is still larger. Therefore miRNAs discovery remains an important aspect understanding this new widely unknown regulation mechanism. Bioinformatics approaches proved be useful toward goal by guiding experimental...
Shwachman-Diamond syndrome (SDS) (OMIM #260400) is a rare inherited bone marrow failure (IBMFS) that primarily characterized by neutropenia and exocrine pancreatic insufficiency. Seventy-five to ninety percent of patients have compound heterozygous loss-of-function mutations in the Shwachman-Bodian-Diamond (sbds) gene. Using trio whole-exome sequencing (WES) an sbds-negative SDS family candidate gene additional SBDS-negative cases or molecularly undiagnosed IBMFS cases, we identified 3...
The drivers of critical coronavirus disease 2019 (COVID-19) remain unknown. Given major confounding factors such as age and comorbidities, true mediators this condition have remained elusive. We used a multi-omics analysis combined with artificial intelligence in young patient cohort where comorbidities were excluded at the onset. included 47 “critical” (in intensive care unit under mechanical ventilation) 25 “non-critical” non-critical ward) patients COVID-19 22 healthy individuals....
Cancer extracellular vesicles (EVs) shuttle at distance and fertilize pre-metastatic niches facilitating subsequent seeding by tumor cells. However, the link between EV secretion mechanisms their capacity to form remains obscure. Using mouse models, we show that GTPases of Ral family control, through phospholipase D1, multi-vesicular bodies homeostasis tune biogenesis pro-metastatic EVs. Importantly, EVs from RalA or RalB depleted cells have limited organotropic capacities in vivo are less...
ABSTRACT Importance Apart from Huntington's disease, little is known of the genetics autosomal dominant chorea associated with dystonia. Here we identify adenylate cyclase 5 ( ADCY5 ) as a likely new causal gene for early‐onset and Observations Whole exome sequencing in three‐generation family affected dystonia identified single de novo mutation—c.2088+1G>A 5' donor splice‐site —segregating disease. This mutation seeming leads to RNA instability therefore haploinsufficiency. Conclusions...
The Nck-associated protein 1–like (NCKAP1L) gene, alternatively called hematopoietic 1 (HEM-1), encodes a lineage–specific regulator of the actin cytoskeleton. Nckap1l-deficient mice have anomalies in lymphocyte development, phagocytosis, and neutrophil migration. Here we report, for first time, NCKAP1L deficiency cases humans. In two unrelated patients Middle Eastern origin, recessive mutations abolishing expression led to immunodeficiency, lymphoproliferation, hyperinflammation with...
Abstract Inherent immune suppression represents a major challenge in the treatment of human cancer. The extracellular matrix molecule tenascin-C promotes cancer by multiple mechanisms, yet roles tumor immunity are incompletely understood. Using 4NQO-induced oral squamous cell carcinoma (OSCC) model with abundant and absent tenascin-C, we demonstrated that enforced an immune-suppressive lymphoid stroma via CCL21/CCR7 signaling, leading to increased metastatic tumors. Through TLR4, expression...
The interplay between cancer cells and immune is a key determinant of tumor survival. Here, we uncovered how tumors exploit the immunomodulatory properties extracellular matrix to create microenvironment that enables their escape from surveillance. Using orthotopic grafting mammary in immunocompetent mice autochthonous models breast cancer, discovered tenascin-C, molecule absent most healthy adult tissues but expressed at high levels associated with poor patient prognosis many solid cancers,...
Abstract Protein‐based virtual screening of chemical libraries is a powerful technique for identifying new molecules that may interact with macromolecular target interest. Because docking and scoring limitations, it more difficult to apply as lead optimization method because requires the docking/scoring tool able propose few solutions possible all them very good accuracy both protein‐bound orientation conformation ligand. In present study, we consensus approach (ConsDock) takes advantage...
Abstract The Protein Data Bank (PDB) has been processed to extract a screening protein library (sc‐PDB) of 2148 entries. A knowledge‐based detection algorithm applied 18,000 PDB files find regular expressions corresponding either protein, ions, co‐factors, solvent, or ligand atoms. sc‐PDB database comprises high‐resolution X‐ray structures proteins for which (i) well‐defined active site exists, (ii) the bound‐ligand is small molecular weight molecule. screened by an inverse docking tool...
In addition to acting as an RNA quality control pathway, nonsense-mediated mRNA decay (NMD) plays roles in regulating normal gene expression. particular, the extent which alternative splicing is coupled NMD and of uORF containing transcripts have been a matter debate.In order achieve greater understanding regulated expression we used 2D-DiGE proteomics technology examine changes protein induced HeLa cells by UPF1 knockdown. QPCR based validation corresponding mRNAs, response both knockdown...
à la diffusion de documents scientifiques niveau recherche, publiés ou non, émanant des établissements d'enseignement et recherche français étrangers, laboratoires publics privés.
Abstract The prognosis of patients with relapsed/refractory (R/R) diffuse large B-cell lymphoma (DLBCL) remains unsatisfactory and, despite major advances in genomic studies, the biological mechanisms underlying chemoresistance are still poorly understood. We conducted for first time a large-scale differential multi-omics investigation on DLBCL patient’s samples order to identify new biomarkers that could early at risk R/R disease and targets determine chemorefractoriness. compared...
Abstract Tumor progression and metastatic dissemination are driven by cell-intrinsic biomechanical cues that favor the growth of life-threatening secondary tumors. We recently identified pro-metastatic vascular regions with blood flow profiles permissive for arrest circulating tumor cells. have further established such also control endothelial remodeling, which favors extravasation arrested CTCs. Yet, how shear forces remodeling is unknown. In present work, we aimed at dissecting cellular...
Abstract Background Gout, which manifests as severe inflammation caused by excessive serum urate levels and monosodium deposits in the joints, is associated with multiple genomic loci Europeans. In this exploratory work, we aimed to identify additional gene variants Melanesian families from New Caledonia, a population disease highly prevalent. Methods Two Lifou Island (New Caledonia) members were diagnosed gout selected. Whole exome sequencing and/or targeted of panel genes involved immunity...
SRC-3 is an important coactivator of nuclear receptors including the retinoic acid (RA) receptor α. Most functions are facilitated by changes in posttranslational code protein that involves mainly phosphorylation and ubiquitination. We recently reported degraded proteasome response to RA. Here, using RNAi E3-ubiquitin ligase entry screen, we identified CUL-3 RBX1 as components E3 ubiquitin involved RA-induced ubiquitination subsequent degradation SRC-3. also show depends on its prior at...
HIV-specific broadly neutralizing antibodies (bnAbs) have been isolated from patients with high viremia but also HIV controllers that repress HIV-1 replication. In these elite (ECs), multiple parameters contribute to viral suppression, including genetic factors and immune responses. Defining the correlates associated generation of bnAbs may help in designing efficient immunotherapies. this study, ECs either positive or negative for HLA-B*57 protective allele, treated HIV-infected...
Eosinophils are leukocytes that released into the peripheral blood in a phenotypically mature state and capable of being recruited tissues response to appropriate stimuli. Eosinophils, traditionally considered cytotoxic effector cells, airways asthma patients where they believed contribute development many features disease. This perception, however, has been challenged by recent findings suggesting eosinophils have also immunomodulatory functions may be involved tissue homeostasis wound...
The association of primary Sjögren's syndrome (pSS) with Major Histocompatibility Complex (MHC) alleles is quintessential MHC-disease associations. Indeed, although disease associations classical HLA class I and II alleles/haplotypes are amply documented, further dissection often prevented by the strong linkage disequilibrium across entire MHC complex. Here we study pSS, not genes, but non-conventional encoded gene, MICA (MHC chain-related gene A). selectively expressed within epithelia,...