A5072659964- Acute Myeloid Leukemia Research
- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- Chronic Myeloid Leukemia Treatments
- Acute Lymphoblastic Leukemia research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Immune Cell Function and Interaction
- Eosinophilic Disorders and Syndromes
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- T-cell and B-cell Immunology
- Protein Degradation and Inhibitors
- Glycosylation and Glycoproteins Research
- Viral-associated cancers and disorders
- Galectins and Cancer Biology
- Cancer Genomics and Diagnostics
- Immunotherapy and Immune Responses
- Immunodeficiency and Autoimmune Disorders
- Monoclonal and Polyclonal Antibodies Research
- Sepsis Diagnosis and Treatment
- Retinoids in leukemia and cellular processes
- Genomic variations and chromosomal abnormalities
- Genetic factors in colorectal cancer
- Immune cells in cancer
- Blood disorders and treatments
- Vasculitis and related conditions
Centre National de la Recherche Scientifique
1998-2025
Hôpitaux Universitaires de Strasbourg
2012-2025
Immunologie, Immunopathologie et Chimie Thérapeutique
2024-2025
Institut de Biologie Moléculaire et Cellulaire
2024-2025
Université de Strasbourg
2013-2024
Inserm
2001-2024
Hôpital d'Hautepierre
2011-2022
Université Paris Cité
1998-2016
Hôpital Européen Georges-Pompidou
2016
EM Strasbourg Business School
2014
Neutrophils extracellular traps (NETs) have recently emerged as a new potential link between inflammation, immunity, and thrombosis could play key role in septic shock-induced disseminated intravascular coagulation (DIC) pathogenesis. The objective of our study was to investigate NETosis DIC.Twenty patients with shock (10 without 10 DIC according JAAM 2006 score) were prospectively included study. Vascular cell activation assessed by microparticle (MP) measurement. investigated at days 1, 3,...
Aims We recently showed that refractory sprue is distinct from coeliac disease, the former being characterized by abnormal intraepithelial T‐lymphocytes expressing a cytoplasmic CD3 chain (CD3c), lacking and CD8 surface expression, showing TCR γ gene rearrangements. To take advantage of phenotype CD3c + − lymphocytes (IEL) in we developed simple method to distinguish disease sprue. Methods results Comparative immunohistochemical studies using anti‐CD3 anti‐CD8 antibodies were applied on...
Abstract Shed membrane microparticles (MPs) are microvesicles generated from the plasma when cells submitted to stress conditions. Although MPs reflect cell state (at least in vitro ), little is known on their protein composition. We describe first set of experiments aiming characterize MP proteome. Two ways triggering formation a T‐lymphocytic line were analyzed using 1‐D gel approach coupled with LC‐MS/MS and results compared those obtained classic preparation. In total, 390 proteins...
Abstract FIP1L1‐PDGFRA‐positive myeloid neoplasm with eosinophilia (F/P+ MN‐eo) is a rare disease: robust epidemiological data are lacking and reported issues scarce, of low sample‐size limited follow‐up. Imatinib mesylate (IM) highly efficient but no predictive factor relapse after discontinuation has yet been identified. One hundred fifty‐one patients F/P+ MN‐eo (143 males; mean age at diagnosis 49 years; annual incidence: 0.18 case per million population) were included in this...
Abstract Defect in apoptosis has been implicated as a major cause of resistance to chemotherapy observed B cell chronic lymphocytic leukaemia (B CLL). This study evaluated the pro-apoptotic effect an anthocyanin-rich dietary bilberry extract (Antho 50) on CLL cells from 30 patients and peripheral blood mononuclear (PBMCs) healthy subjects determined underlying mechanism. Antho 50 induced concentration- time-dependent effects but little or no PBMCs. Among main phenolic compounds extract,...
Disseminated intravascular coagulation (DIC) is a severe complication of septic shock. Polymorphonuclear neutrophils (PMNs) may play key role in shock-induced DIC via the release extracellular traps (NETs). NETs capture invading pathogens, but also act as pro-coagulant surface at interface between immunity and thrombosis. During DIC, neutrophil activation result excessive NET formation. Herein, we originally report presence circulating human blood during DIC. To investigate formation were...
Abstract The prognosis of patients with relapsed/refractory (R/R) diffuse large B-cell lymphoma (DLBCL) remains unsatisfactory and, despite major advances in genomic studies, the biological mechanisms underlying chemoresistance are still poorly understood. We conducted for first time a large-scale differential multi-omics investigation on DLBCL patient’s samples order to identify new biomarkers that could early at risk R/R disease and targets determine chemorefractoriness. compared...
The diagnosis of mature B-cell neoplasms (MBCN) remains difficult in a number cases, especially leukemic phases non-Hodgkin lymphoma, for which discriminating criteria or biomarker are often lacking. To identify new surface biomarkers, we developed an original proteomic approach based on mass spectrometry analysis plasma membrane microparticles derived from chronic lymphoproliferations single patients: lymphocytic leukemia (CLL), small cell lymphoma (SLL) and mantle (MCL). A straightforward...
To investigate the contribution of neutrophil activation as innate immune cells during septic shock-induced disseminated intravascular coagulation.Prospective study.One University Hospital ICU.Hundred patients with shock. Thirty-five had coagulation according to Japanese Association for Acute Medicine 2006 score.None.Neutrophil chromatin decondensation was assessed by measuring fluorescence (NEUT-side-fluorescence light) labeled a fluorochrome-based polymethine reagent using routine...
Abstract A chronic antigenic stimulation is believed to sustain the leukemogenic development of lymphocytic leukemia (CLL) and most lymphoproliferative malignancies developed from mature B cells. Reproducing a proliferative ex vivo critical decipher mechanisms leukemogenesis in these malignancies. However, functional studies CLL cells remains limited since current cell receptor (BCR) protocols are not sufficient induce proliferation cells, pointing out need mandatory BCR co-factors this...
In order to characterize the genetic diversity in splenic lymphoma with villous lymphocytes (SLVL), we have undertaken cytogenetic and molecular analyses of CCND1 expression BCL1‐IgH PCR rearrangement 76 cases diagnosed predominantly on morphological criteria. Cytogenetic abnormalities were detected 19/44 (43%) cases, including 16/25 (64%) an absolute lymphocytosis. Abnormalities included those involving chromosome 14q32 (9/19, 47%), t(11;14)(q13;q32) (5/19, 26%), 3 (26%), 3q, 17p (26%)...
Toll-like receptors play crucial roles in the sepsis-induced systemic inflammatory response. Septic shock mortality correlates with overexpression of neutrophilic TLR2 and TLR9, while role TLR4 remains a debate. In addition, TLRs are involved pathogenesis viral infections such as COVID-19, where single-stranded RNA SARS-CoV-2 is recognized by TLR7 TLR8, spike protein activates TLR4.
The accurate diagnosis of the different forms chronic mature B-cell lymphocytic malignancies is primary importance to determine an appropriate and efficient treatment. Usually, achieved by morphology immunophenotyping. Nevertheless, diagnostic tools available are not able discriminate pathologies with variable evolution, or classify some them. To discover new biomarkers, we used peptide protein profiling SELDI-TOF−MS, analyze 39 20 control serum samples. Markers interest were subsequently...
Ruxolitinib is a JAK-1/JAK-2 inhibitor indicated for the treatment of polycythemia vera and primary or secondary myelofibrosis. Only one patient (0.2%) was diagnosed with tuberculosis among 485 patients receiving ruxolitinib in four pivotal trials. Fourteen cases have since been reported. We observed two (3%) mycobacterial infections (one due to Mycobacterium avium complex) our cohort 65 ruxolitinib. This observation suggests that rate infection might be higher than trials atypical can also occur.