A5044663343- Bone and Dental Protein Studies
- Cell Adhesion Molecules Research
- Bone Metabolism and Diseases
- dental development and anomalies
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Oral and Maxillofacial Pathology
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Digestive system and related health
- Immune cells in cancer
- Cytokine Signaling Pathways and Interactions
- Protease and Inhibitor Mechanisms
- Bone health and treatments
- Cellular Mechanics and Interactions
- interferon and immune responses
- NF-κB Signaling Pathways
- RNA Research and Splicing
- Corneal Surgery and Treatments
- Signaling Pathways in Disease
- Vitamin D Research Studies
- Connexins and lens biology
- Platelet Disorders and Treatments
- Chemokine receptors and signaling
- Monoclonal and Polyclonal Antibodies Research
Fukuyama University
2016-2025
Hokkaido University
2009-2020
Hokkaido Pharmaceutical University
2014-2017
University of California, San Francisco
2011
Center of Molecular Immunology (Cuba)
2009
Dana-Farber Cancer Institute
2008
University of Basel
2005
University of Freiburg
2005
Universität Ulm
2005
Medical & Biological Laboratories (Japan)
2001-2005
Rheumatoid arthritis is one of the most critical diseases that impair quality life patients, but its pathogenesis has not yet been fully understood. Osteopontin (OPN) an extracellular matrix protein containing Arg-Gly-Asp (RGD) sequence, which interacts with αvβ3 integrins, promotes cell attachment, and migration expressed in both synovial cells chondrocytes rheumatoid arthritis; however, functional relationship to known. Therefore, we investigated roles OPN inflammatory process a model...
Osteopontin (OPN), a noncollagenous adhesive protein, is implicated in atherosclerosis, which macrophages within atherosclerotic plaques express OPN. However, it not known whether the elevated OPN expression cause or result of atherosclerosis.We generated mice that lacked and crossed them with apolipoprotein (apo) E-deficient analyzed these mixed C57BL/6x129 background after 36 weeks on normal chow diet. In female mice, OP+/-E-/- OP-/-E-/- had significantly smaller inflammatory lesions...
Osteopontin (OPN) is upregulated in several experimental models of cardiac fibrosis and remodeling. However, its direct effects remain unclear. We examined the hypothesis that OPN important for development Moreover, we whether inhibitory effect eplerenone (Ep), a novel aldosterone receptor antagonist, was mediated through inhibition expression against Wild-type (WT) OPN-deficient mice were treated with angiotensin II (Ang II) 4 weeks. WT receiving Ang divided into 2 groups: control group an...
It has been shown that osteopontin (OPN) plays a pivotal role in the pathogenesis of rheumatoid arthritis (RA). However, molecular mechanism OPN action is yet to be elucidated. Splenic monocytes obtained from arthritic mice exhibited significant capacity for cell migration toward thrombin-cleaved but not full-length OPN. Migratory expressed alpha9 and alpha4 integrins. Since cleavage by thrombin exposes cryptic epitope recognized integrins, we investigated SLAYGLR murine RA model using...
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Tyrosine kinase 2 (Tyk2), a member of the Jak family, mediates signals triggered by various cytokines, which are related to pathogenesis psoriasis. In this study, we investigated role Tyk2 in IL-23-induced psoriasis-like skin inflammation. Tyk2(-/-) mice when injected with IL-23 showed significantly reduced ear swelling epidermal hyperplasia and inflammatory cell infiltration compared wild-type mice. addition, deficiency production pro-inflammatory cytokines psoriasis-relevant anti-microbial...
In psoriasis lesions, a diverse mixture of cytokines is up-regulated that influence each other generating complex inflammatory situation. Although this the case, inhibition IL-17A alone showed unprecedented clinical results in patients, indicating critical inducer pathogenesis. To elucidate IL-17A-driven keratinocyte-intrinsic signaling pathways, we treated monolayers normal human epidermal keratinocytes vitro with six (IL-17A, TNF-α, IL-17C, IL-22, IL-36γ and IFN-γ) involved to mimic milieu...
Abstract Inherent immune suppression represents a major challenge in the treatment of human cancer. The extracellular matrix molecule tenascin-C promotes cancer by multiple mechanisms, yet roles tumor immunity are incompletely understood. Using 4NQO-induced oral squamous cell carcinoma (OSCC) model with abundant and absent tenascin-C, we demonstrated that enforced an immune-suppressive lymphoid stroma via CCL21/CCR7 signaling, leading to increased metastatic tumors. Through TLR4, expression...
Osteopontin (OPN) is a chemotactic protein that attracts immune cells, to inflammatory sites. The sensitization phase of allergic cutaneous contact hypersensitivity (CHS) requires the migration Langerhans cells/dendritic cells (LCs/DCs) from skin draining lymph nodes. Characterizing OPN function for LC/DC we found upregulated expression in hapten sensitized and induces migration, initiates their emigration epidermis, LCs/DCs nodes by interacting with CD44 αv integrin. Furthermore,...
Tumor growth and metastasis are angiogenesis-dependent processes initiated regulated by a number of cytokines. Vascular endothelial factor (VEGF) is potent angiogenic protein with selective mitogenic effect on vascular cells. Osteopontin (OPN) induces cell migration upregulates induced VEGF. To clarify the cooperative role VEGF OPN in tumor angiogenesis, we stained VEGF, OPN, CD34 immunohistochemically 87 cases stage I non-small lung cancer (adenocarcinoma, 55, squamous carcinoma, 32). Of...
Abstract —We have previously reported that high glucose stimulates osteopontin (OPN) expression through protein kinase C–dependent pathways as well hexosamine in cultured rat aortic smooth muscle cells. The finding prompted us to study vivo of OPN diabetes mellitus. In the present study, we found by immunohistochemistry medial layers carotid arteries streptozotocin-induced diabetic rats and forearm patients stained positively for antibodies, whereas staining from control nondiabetic was...
Osteopontin (OPN), originally discovered in bone as an extracellular matrix protein, was identified many cell types the immune system, presumably being involved aspects of pathogenesis inflammatory and diseases. Mast cells are also such pathological by secreting multiple mediators. However, it has not been determined whether mast produce OPN affects their function. To test this, we used murine fetal skin-derived cultured (FSMC) marrow-derived cells. We found that spontaneously produced FSMC...
Osteopontin is critically involved in rheumatoid arthritis; however, the molecular cross-talk between osteopontin and joint cell components that leads to inflammatory destruction largely unknown. We found not only but also tenascin-C their common receptor, alpha(9) integrin, are expressed at arthritic joints. The local production of mainly due synovial fibroblasts and, a lesser extent, macrophages. Synovial macrophages express autocrine paracrine interactions integrin on its ligands...
To investigate the role of osteopontin (OPN) in development osteoarthritis (OA) under vivo and vitro conditions.Both instability-induced aging-associated OA models were generated using OPN-deficient (OPN-/-) control wild-type (WT) mice. An cartilage degradation model was also used, to evaluate effect OPN on proteoglycan loss from joint cartilage.OPN deficiency exacerbated both OA. Both structural changes an increased tissue augmented absence OPN. led induction matrix metalloproteinase 13...
Currently, there is no effective intervention available that can reduce brain damage following reperfusion. Clinical studies suggest a positive correlation between the increased influx of neutrophils and severity injury Integrin α9β1 highly expressed on activated contributes to stable adhesion, but its role in stroke outcome has not been demonstrated date.
We have generated synthetic peptides corresponding to various portions of human osteopontin (OPN) and immunized rabbits mice with these generate polyclonal monoclonal antibodies specific OPN. then six distinct sandwich enzyme-linked immunoabsorbent assay (ELISA) systems by using different pairs against These allowed us detect not only isoforms truncated forms recombinant OPN, but also the glycosylated form native urinary Most importantly, tumor-derived OPN was differentially detected ELISA...
Abstract Purpose: The development of hepatocellular carcinoma is associated with the chronic inflammation liver caused by various factors such as hepatitis B or C virus infection. Previously, we reported DNA binding protein A (dbpA) a candidate molecule that can accelerate inflammation-induced hepatocarcinogenesis. DbpA belongs to Y-box family, and protein-1 (YB-1), prototype member this be prognostic marker malignant diseases other than carcinoma. purpose study examine significance...
purpose. To investigate the effects of loss osteopontin (OPN) in healing injured cornea mice. Cell culture study was also conducted to clarify OPN fibroblast behaviors. methods. Ocular fibroblasts from wild-type (WT) and OPN-null (KO) mice were used role on cell behavior. The effect lack corneal wound evaluated results. In culture, is involved adhesion migration ocular fibroblasts. Adhesion epithelial line not affected by exogenous OPN. upregulated a healing, mouse cornea. Loss did affect...
The balance between immune activation and suppression must be regulated to maintain homeostasis. Tissue macrophages (MΦs) constitute the major cellular subsets of APCs within body; however, how what types resident MΦs are involved in regulation homeostasis peripheral lymphoid tissues poorly understood. Splenic red pulp MΦ (RPMs) remove self-Ags, such as blood-borne particulates aged erythrocytes, from blood. Although many scattered T cells exist spleen, little attention has been given RPMs...
We recently reported that Lactobacillus helveticus SBT2171 (LH2171) inhibited the proliferation and inflammatory cytokine production of primary immune cells in vitro, alleviated collagen-induced arthritis (CIA) mice, a model human rheumatoid (RA). In this study, we newly investigated whether LH2171 could relieve severity experimental autoimmune encephalomyelitis (EAE), murine multiple sclerosis (MS), which is an disease, but develop symptoms by different mechanisms from RA. MS EAE, main...