A5040756693- Cell Adhesion Molecules Research
- Platelet Disorders and Treatments
- Complement system in diseases
- Blood Coagulation and Thrombosis Mechanisms
- Atherosclerosis and Cardiovascular Diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Acute Ischemic Stroke Management
- Venous Thromboembolism Diagnosis and Management
- S100 Proteins and Annexins
- Neuroinflammation and Neurodegeneration Mechanisms
- Protease and Inhibitor Mechanisms
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cancer, Hypoxia, and Metabolism
- Antiplatelet Therapy and Cardiovascular Diseases
- Pancreatic and Hepatic Oncology Research
- Immune cells in cancer
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Peroxisome Proliferator-Activated Receptors
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Radiomics and Machine Learning in Medical Imaging
- Nitric Oxide and Endothelin Effects
- Cerebrovascular and Carotid Artery Diseases
- Neurological Disorders and Treatments
- Glaucoma and retinal disorders
University of Iowa
2016-2025
Carver Bible College
2025
Post Graduate Institute of Medical Education and Research
2022-2024
Banaras Hindu University
2017-2023
Institut d'Investigacions Biomèdiques de Barcelona
2023
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2023
Biomedical Research Institute
2023
National Academies of Sciences, Engineering, and Medicine
2023
Consejo Superior de Investigaciones Científicas
2023
Universitat de Barcelona
2023
A heparin binding mitogenic protein isolated from bovine uterus shares NH 2 -terminal amino acid sequence with a newborn rat brain. The cDNA's of the bovine, human, and genes have been encode extraordinarily conserved proteins unrelated to known growth or neurotrophic factors, although identity nearly 50 percent has found predicted retinoic induced transcript in differentiating mouse embryonal carcinoma cells. Lysates COS-7 cells transiently expressing this were for NRK initiated neurite...
There is a critical need for cerebro-protective interventions to improve the suboptimal outcomes of patients with ischemic stroke who have been treated reperfusion strategies. We found that nuclear pyruvate kinase muscle 2 (PKM2), modulator systemic inflammation, was upregulated in neutrophils after onset both humans and mice. Therefore, we determined role PKM2 pathogenesis by using murine models preexisting comorbidities. generated novel myeloid cell-specific PKM2-/- mice on wild-type...
The glycolytic enzyme PKM2 (pyruvate kinase muscle 2) is upregulated in monocytes/macrophages of patients with atherosclerotic coronary artery disease. However, the role cell type-specific setting atherosclerosis remains to be defined. We determined whether myeloid cell-specific regulates efferocytosis and atherosclerosis.
Thrombotic thrombocytopenic purpura (TTP) is a life-threatening illness caused by deficiency of the vWF-cleaving protease ADAMTS13. Here we show that ADAMTS13-deficient mice are viable and exhibit normal survival, although vWF-mediated platelet-endothelial interactions significantly prolonged. Introduction genetic background CASA/Rk (a mouse strain with elevated plasma vWF) resulted in appearance spontaneous thrombocytopenia subset decreased survival. Challenge these shigatoxin (derived from...
Fibronectins (FNs) are multifunctional high molecular weight glycoproteins present in the blood plasma and ECMs of tissues. The FN primary transcript undergoes alternative splicing three regions generating up to 20 main different variants humans. However, precise role isoforms is poorly understood. One alternatively spliced exons extra domain A (EDA) or type III homology that regulated spatially temporally during development aging. To study its vivo function, we generated mice devoid EDA...
The metalloprotease ADAMTS13 (a disintegrin-like and with thrombospondin type I repeats 13) cleaves highly adhesive large von Willebrand factor (VWF) multimers after their release from the endothelium. deficiency is linked to a life-threatening disorder, thrombotic thrombocytopenic purpura (TTP), characterized by platelet-rich thrombi in microvasculature. Here, we show spontaneous thrombus formation activated microvenules of Adamts13−/− mice intravital microscopy. Strikingly, found that...
von Willebrand factor (VWF) levels are elevated and a disintegrin-like metalloprotease with thrombospondin type I repeats-13 (ADAMTS13) activity is decreased in both acute chronic inflammation. We hypothesized that by cleaving hyperactive ultralarge VWF (ULVWF) multimers, ADAMTS13 down-regulates thrombosis Using intravital microscopy, we show deficiency results increased leukocyte rolling on unstimulated veins adhesion inflamed veins. Both processes were dependent the presence of VWF....
Background— The incidence of thrombotic events increases during aging, but the mechanisms are not well understood. To investigate prothrombotic role oxidative stress we tested hypothesis that aged mice overexpressing antioxidant enzyme glutathione peroxidase-1 (Gpx1) protected from experimental thrombosis. Methods and Results— Susceptibility to carotid artery thrombosis was first examined in wild-type C57BL/6J mice. After photochemical injury artery, time stable occlusion significantly...
Mitochondrial fission and fusion impact numerous cellular functions neurons are particularly sensitive to perturbations in mitochondrial dynamics. Here we describe that male mice lacking the A-kinase anchoring protein 1 (AKAP1) exhibit increased sensitivity transient middle cerebral artery occlusion model of focal ischemia. At ultrastructural level, AKAP1 −/− have smaller mitochondria contacts between endoplasmic reticulum brain. Mechanistically, deletion dysregulates complex II electron...
Background/Aims: Treating hepatocellular carcinoma (HCC) with portal vein tumor thrombosis (PVTT) remains controversial. We compared the outcomes of hepatic resection (HR), transarterial chemoembolization (TACE), and sorafenib therapy as treatments for HCC PVTT. Methods: Patients diagnosed PVTT between January 2000 December 2011 who received treatment sorafenib, HR, or TACE were included. main PVTT, superior mesenteric thrombosis, Child-Turcotte-Pugh (CTP) class C excluded. The records 172...
Human diseases may be modeled in animals to allow preclinical assessment of putative new clinical interventions. Recent, highly publicized failures large trials called into question the rigor, design, and value assessment. We established Stroke Preclinical Assessment Network (SPAN) design implement a randomized, controlled, blinded, multi-laboratory trial for rigorous candidate stroke treatments combined with intravascular thrombectomy. Efficacy futility boundaries multi-arm multi-stage...
Respiratory tract infections (RTIs) caused by bacteria or viruses are associated with stroke severity. Recent studies have revealed an imbalance in the von Willebrand factor (VWF)-ADAMTS13 axis patients RTIs, including COVID-19. We examined whether this contributes to RTI-mediated Wild-type (WT), Vwf −/−, Adamts13−/− mice respective littermate controls (Vwf +/+, Adamts13+/+) were infected intranasally sublethal doses of S. aureus (on days 0, 2, and 5) mouse-adapted SARS-CoV-2 day 0)...
The pleiotrophin (PTN) gene (Ptn) encodes an 18-kDa protein that is highly conserved among mammalian species and functions as a weak mitogen promotes neurite-outgrowth activity in vitro. To further investigate the role PTN plays regulating cell growth, we overexpressed bovine cDNA now show phenotypically transforms NIH 3T3 cells, evidenced by increased number at confluence, focus formation, anchorage-independent tumor formation nude mouse. results demonstrate Ptn has potential to regulate...
The peroxiredoxin (Prdx) family of antioxidant enzymes uses redox-active cysteines to reduce peroxides, lipid hydroperoxides, and peroxynitrites. Prdx1 is known be important protect red blood cells against reactive oxygen species in tumor prevention. In this study, the role inflammation, thrombosis, atherosclerosis was investigated. Using intravital microscopy, we showed that number leukocytes rolling per minute unstimulated veins increased by 2.5-fold Prdx1(-/-) compared Prdx1(+/+) mice....
The origin of the fibronectin (FN) found in extracellular matrix tissues has not been defined experimentally. Previous studies suggest that there is contribution from both local tissue production and transfer plasma, but extent this phenomenon addressed. We have shown before engineered mice constitutively expressing extra domain A-containing FN (EDA+FN) a significant decrease levels plasma most tissues. showed hepatocytes modified to produce EDA+FN normal matrix-FN secrete less soluble FN....
Oxidative stress accompanies inflammatory and vascular diseases. The objective of this study was to explore whether reactive oxygen species can activate shedding platelet receptors thus suppress function. Hydrogen peroxide glucose oxidase were chosen model oxidative in vitro. We demonstrate that damage activated tumour necrosis factor-α-converting enzyme (TACE) induced its targets, glycoprotein (GP) Ibα GPV, murine human platelets. Also, 12-HpETE, a synthesized the lipoxygenase pathway,...