A5075940380- MicroRNA in disease regulation
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Circular RNAs in diseases
- PI3K/AKT/mTOR signaling in cancer
- Parasite Biology and Host Interactions
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- IL-33, ST2, and ILC Pathways
- Mosquito-borne diseases and control
- Insect Resistance and Genetics
- Parasites and Host Interactions
- T-cell and Retrovirus Studies
- Cytokine Signaling Pathways and Interactions
- Systemic Sclerosis and Related Diseases
- Tendon Structure and Treatment
- Plant Molecular Biology Research
- Muscle Physiology and Disorders
- Bone and Joint Diseases
- interferon and immune responses
- Helminth infection and control
- Insect symbiosis and bacterial influences
- Genetics, Aging, and Longevity in Model Organisms
- CRISPR and Genetic Engineering
- Endoplasmic Reticulum Stress and Disease
Yale University
2017-2020
University of Aberdeen
2013-2019
Institut de Biologie Moléculaire des Plantes
2015
Université de Strasbourg
2011-2015
Immuno-Rhumathologie moléculaire
2014
Institute of Medical Sciences
2013
Inserm
2012
Hôpitaux Universitaires de Strasbourg
2011
Highlights•mTORC1 transcriptionally regulates amino acid transporters and enzymes via ATF4•mTORC1 controls ATF4 by regulating the translation stability of its mRNA•Control requires uORFs, but not changes in eIF2α phosphorylation•mTORC1 control instead employs 4E-BP repressorsSummaryThe mammalian target rapamycin complex 1 (mTORC1) is a master regulator cell growth that commonly deregulated human diseases. Here we find mTORC1 transcriptional program encoding metabolic through mechanism also...
Terminal oligopyrimidine (TOP) motifs are sequences at the 5' ends of mRNAs that link their translation to mTOR Complex 1 (mTORC1) nutrient-sensing signaling pathway. They commonly regarded as discrete elements reside on ∼100 mostly encode factors. However, full spectrum TOP and prevalence throughout transcriptome remain unclear, primarily because uncertainty over mechanism detects them. Here, we globally analyzed targets La-related protein (LARP1), an RNA-binding mTORC1 effector has been...
Resident cells, such as fibroblast-like synoviocytes (FLS), play a crucial role in rheumatoid arthritis (RA). They are implicated the inflammatory response and key osteoarticular destruction. Moreover, RA FLS spread to unaffected joints. Pathogen-associated molecular patterns damage-associated have been found activate by interacting with pattern recognition receptors, TLR. express large number of TLR, TLR2 was demonstrated be involved inflammation. Because microRNA emerged important...
Cell growth is a complex process shaped by extensive and coordinated changes in gene expression. Among these the tightly regulated translation of family growth-related mRNAs defined 5' terminal oligopyrimidine (TOP) motif. TOP mRNA partly controlled via eukaryotic initiation factor 4F (eIF4F), that recognizes cap structure. Recent studies have also implicated La-related protein 1 (LARP1), which competes with eIF4F for binding to ends. However, it has remained controversial whether LARP1...
Objective To evaluate whether miR-20a belonging to the cluster miR-17–92 is a negative regulator of inflammation in rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS) by modulating expression apoptosis signal-regulating kinase (ASK) 1, key component toll-like receptors 4 pathway, upstream p38 mitogen-activated protein kinase. Methods Evaluation and ASK1 mRNA was performed RT-qPCR. assessed western blotting. Overexpression transfection RA FLS THP-1 cells with mimics. Interleukin...
We evaluated micro (mi) RNA-mediated regulation of BAFF expression in fibroblasts using two concomitant models: (i) synovial (FLS) isolated from healthy controls (N) or Rheumatoid Arthritis (RA) patients; (ii) human dermal (HDF) Systemic Sclerosis (SSc) patients. Using RT-qPCR and ELISA, we first showed that SScHDF synthesized released response to Poly(I:C) IFN-γ treatment, as previously observed RAFLS, whereas NHDF preferentially IFN-γ. Next, demonstrated miR-30a-3p was down regulated RAFLS...
MicroRNAs (miRNAs) are now recognized as essential regulators of gene expression in plants and animals. They potentially modulate the multiple genes thereby enabling homeostatic settings physiological conditions. Their role is also increasingly considered many diseases which deregulated epigenetic mechanisms induce aberrant expression. Work conducted our laboratory has recently led to identification miRNAs for control inflammatory reactions that occur during rheumatoid arthritis. In this...
The biosynthesis of ribosomal RNA and its incorporation into functional ribosomes is an essential intricate process that includes production mature from large precursors. Here, we analyse the contribution plant exosome co-factors to processing degradation 18S pre-RNAs in Arabidopsis thaliana. Our data show that, unlike yeast humans, RRP6 homologue, nucleolar exoribonuclease RRP6L2, complex, together with RRP44, function two distinct steps pre-18S rRNA or Arabidopsis. In addition, identify...
The organization of genes into operons, clusters that are co-transcribed to produce polycistronic pre-mRNAs, is a trait found in wide range eukaryotic groups, including multiple animal phyla. Operons present the class Chromadorea, one two main nematode classes, but their distribution other class, Enoplea, not known. We have surveyed genomes Trichinella spiralis, Trichuris muris, and Romanomermis culicivorax identified first putative operons members Enoplea. Consistent with mechanism RNA...
Spliced leader (SL) trans-splicing is a critical element of gene expression in number eukaryotic groups. This process arguably best understood nematodes, where biochemical and molecular studies Caenorhabditis elegans Ascaris suum have identified key steps factors involved. Despite this, the precise details SL yet to be elucidated. In part, this because systematic identification molecules involved has not previously been possible due lack specific phenotype associated with defects process. We...
Infections with parasitic nematodes are among the most significant of neglected tropical diseases affecting about a billion people living mainly in regions low economic activity. The effective current strategy to control nematode infections involves large scale treatment programs anthelmintic drugs. This is at risk from emergence drug resistant parasites. Parasitic also affect livestock, which treated same limited group Livestock parasites single drugs, and even multi-drug parasites,...
Over the past decade, microRNAs (miRNAs) have emerged as major players enabling accurate gene expression and regulation. As such, they participate in basic cellular processes, like apoptosis proliferation which are often deregulated cancer cells (1). This feature has been largely documented case of miR-17~92 cluster, overexpression is a key event oncogenesis (2).