A5031559798- Viral Infectious Diseases and Gene Expression in Insects
- Transgenic Plants and Applications
- Insect Resistance and Genetics
- CRISPR and Genetic Engineering
- Blood groups and transfusion
- Endoplasmic Reticulum Stress and Disease
- Immune Response and Inflammation
- Protein purification and stability
- Pluripotent Stem Cells Research
- RNA and protein synthesis mechanisms
- Immunodeficiency and Autoimmune Disorders
- RNA Interference and Gene Delivery
- Insect symbiosis and bacterial influences
- Pneumonia and Respiratory Infections
- NF-κB Signaling Pathways
- Advanced biosensing and bioanalysis techniques
- Insect Pest Control Strategies
- interferon and immune responses
- Circadian rhythm and melatonin
- Insect and Pesticide Research
- Genetics, Aging, and Longevity in Model Organisms
Institut de génétique et de biologie moléculaire et cellulaire
2020-2024
Centre National de la Recherche Scientifique
2020-2024
Université de Strasbourg
2020-2024
Inserm
2020-2024
Institut de Biologie Moléculaire et Cellulaire
2022
Integrated Structural Biology Grenoble
2021
The cuticle of C. elegans is impermeable to chemicals, toxins, and pathogens. However, increased permeability a desirable phenotype because it facilitates chemical uptake. Surface lipids contribute the barrier. Here, we identify lipid transfer protein GMAP-1 as critical element setting cuticle. A gmap-1 deletion mutant increases cuticular sodium azide, levamisole, Hoechst, DiI. Expressing in hypodermis or transiently adults sufficient rescue this phenotype. secreted from aqueous fluid...
Abstract The Baculovirus/insect cell expression system is a powerful technology for reconstitution of eukaryotic macromolecular assemblies. Most multigene platforms rely on Tn7-mediated transposition transferring the cassette into baculoviral genome. This allows rigorous characterization recombinant bacmids but involves multiple steps, limitation when many constructs are to be tested. For parallel screening and potential high throughput applications, we have established an open source...
The nematode cuticle is a thick and resistant hydroskeleton that acts as barrier controlling exchanges between the external environment internal medium. of C. elegans- free-living - impermeable to chemicals, toxins pathogens. Increased permeability desirable phenotype because it facilitates chemical uptake, leading increased sensitivity agents reduced risks false negatives during small molecule screening. Permeability defects can arise from deficiencies in subset collagens, surface coat...