A5047764521- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Biochemical and Molecular Research
- Atherosclerosis and Cardiovascular Diseases
- Nitric Oxide and Endothelin Effects
- Parasitic Diseases Research and Treatment
- Insect symbiosis and bacterial influences
- Vector-borne infectious diseases
- Lysosomal Storage Disorders Research
- Monoclonal and Polyclonal Antibodies Research
- Toxin Mechanisms and Immunotoxins
- Parasites and Host Interactions
- Insect and Pesticide Research
- Vector-Borne Animal Diseases
- vaccines and immunoinformatics approaches
- Parasite Biology and Host Interactions
- Advanced Glycation End Products research
- Plant Virus Research Studies
- Signaling Pathways in Disease
- Virus-based gene therapy research
- Galectins and Cancer Biology
- Calcium signaling and nucleotide metabolism
- MicroRNA in disease regulation
- Parasitic Infections and Diagnostics
Université Libre de Bruxelles
2016-2025
Institute of Pathology and Genetics
2005-2023
Institut des Biomolécules Max Mousseron
2020
MRC Laboratory of Molecular Biology
2010
In-Q-Tel
2008
Molina Center for Energy and the Environment
2008
Trinity College Dublin
2005
Gembloux Agro-Bio Tech
2005
Instituut voor Tropische Geneeskunde
1997
University of Cambridge
1997
Abstract Onchocerciasis is a parasitic disease with high socio-economic burden particularly in sub-Saharan Africa. The elimination plan for this has faced numerous challenges. A multi-epitope prophylactic/therapeutic vaccine targeting the infective L3 and microfilaria stages of parasite’s life cycle would be invaluable to achieve current goal. There are several observations that make possibility developing against likely. For example, despite being exposed transmission rates infection, 1 5%...
Apolipoprotein L-I is the trypanolytic factor of human serum. Here we show that this protein contains a membrane pore-forming domain functionally similar to bacterial colicins, flanked by membrane-addressing domain. In lipid bilayer membranes, apolipoprotein formed anion channels. Trypanosoma brucei, was targeted lysosomal and triggered depolarization membrane, continuous influx chloride, subsequent osmotic swelling lysosome until trypanosome lysed.
Abstract Background MicroRNAs (miRNAs) are small (19-22-nt) single-stranded noncoding RNA molecules whose deregulation of expression can contribute to human disease including the multistep processes carcinogenesis in human. Circulating miRNAs emerging biomarkers many diseases and cancers such as type 2 diabetes, pulmonary disease, colorectal cancer, gastric cancer among others; however, defining a plasma miRNA signature acute myeloblastic leukemia (AML) that could serve biomarker for...
In the search for new diagnostic methods that would distinguish Trypanosoma brucei rhodesiense from T. b. and gambiense, we have developed two polymerase chain reaction (PCR) primer sets. The first set was derived serum resistance-associated (SRA) gene of confers resistance to lysis by normal human (NHS). specificity SRA-based PCR tested on 97 different trypanosome populations originating various taxonomic groups, host species, geographic regions. Only one 25 samples negative in this PCR,...
The protozoan parasite Trypanosoma brucei develops antigenic variation to escape the immune response of its host. To this end, trypanosome genome contains multiple telomeric expression sites competent for transcription variant surface glycoprotein genes, but as a rule only single antigen is expressed at any time. We used reverse transcription‐PCR (RT‐PCR) analyse different segments in clones two independent strains T. . results indicated that RNA polymerase installed and active beginning...
Background During their blood meal, ticks secrete a wide variety of proteins that can interfere with host's defense mechanisms. Among these proteins, lipocalins play major role in the modulation inflammatory response. Methodology/Principal Findings We previously identified 14 new lipocalin genes tick Ixodes ricinus. One them codes for protein specifically binds leukotriene B4 very high affinity (Kd: ±1 nM), similar to neutrophil transmembrane receptor BLT1. By silico approaches, we modeled...
Blood coagulation starts immediately after damage to the vascular endothelium. This system is essential for minimizing blood loss from an injured vessel but also contributes thrombosis. Although it has long been thought that intrinsic pathway not important clotting in vivo, recent data obtained with genetically altered mice indicate contact phase proteins seem be thrombus formation. We show recombinant Ixodes ricinus inhibitor (Ir-CPI), a Kunitz-type protein expressed by salivary glands of...
Serpins (serine protease inhibitors) are a large family of structurally related proteins found in wide variety organisms, including hematophagous arthropods. Protein analyses revealed that Iris, previously described as an immunomodulator secreted the tick saliva, is to leukocyte elastase inhibitor and possesses serpin motifs, reactive center loop (RCL), which involved interaction between serpins serine proteases. Only proteases were inhibited by purified recombinant Iris (rIris), whereas...
BackgroundTicks are blood feeding arachnids that characteristically take a long meal. They must therefore counteract host defence mechanisms such as hemostasis, inflammation and the immune response. This is achieved by expressing batteries of salivary proteins coded multigene families.Methodology/Principal FindingsWe report in-depth analysis tick family describe five new anticomplement in Ixodes ricinus. Compared to previously described proteins, these segregated into phylogenetic group or...
Lipocalins are widely distributed in nature and found bacteria, plants, arthropoda vertebra. In hematophagous arthropods, they implicated the successful accomplishment of blood meal, interfering with platelet aggregation, coagulation inflammation transmission disease parasites such as Trypanosoma cruzi Borrelia burgdorferi. The pairwise sequence identity is low among this family, often below 30%, despite a well conserved tertiary structure. Under 30% threshold, alignment methods do not...
Oxidation of LDL by the myeloperoxidase (MPO)-H2O2-chloride system is a key event in development atherosclerosis. The present study aimed at investigating interaction MPO with native and modified revealing posttranslational modifications on apoB-100 (the unique apolipoprotein LDL) vitro vivo. Using amperometry, we demonstrate that activity increases up to 90% when it adsorbed surface LDL. This phenomenon apparently reflected local structural changes observed circular dichroism. MS, further...