A5102706406- Hematopoietic Stem Cell Transplantation
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Chronic Myeloid Leukemia Treatments
- Chronic Lymphocytic Leukemia Research
- Acute Lymphoblastic Leukemia research
- T-cell and B-cell Immunology
- Acute Myeloid Leukemia Research
- Mesenchymal stem cell research
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Cytomegalovirus and herpesvirus research
- Renal Transplantation Outcomes and Treatments
- Lymphoma Diagnosis and Treatment
- Extracellular vesicles in disease
- Polyomavirus and related diseases
- Viral-associated cancers and disorders
- Virus-based gene therapy research
- Eosinophilic Disorders and Syndromes
- Cancer-related molecular mechanisms research
- Transplantation: Methods and Outcomes
- Bone and Joint Diseases
- Animal Disease Management and Epidemiology
- Tissue Engineering and Regenerative Medicine
Institut Jules Bordet
2015-2024
Université Libre de Bruxelles
2015-2024
Rega Institute for Medical Research
2022
KU Leuven
2022
Lebanese University
2011-2020
Palmetto Hematology Oncology
2019
Universitätsklinikum Aachen
2017
Amis de l'Institut Bordet
2010
Laboratory of Molecular Genetics
2000
Cliniques Universitaires Saint-Luc
1993-1997
Treg are the main mediators of dominant tolerance. Their mechanisms action and applications subjects considerable debate currently. However, a human microRNA (miR) signature has not been described yet. We investigated natural identified composed five miR (21, 31, 125a, 181c 374). Among those, two were considerably under-expressed (miR-31 miR-125a). functional target sequence for miR-31 in 3' untranslated region (3' UTR) FOXP3 mRNA. Using lentiviral transduction fresh cord blood T cells, we...
Abstract Background MicroRNAs (miRNAs) are small (19-22-nt) single-stranded noncoding RNA molecules whose deregulation of expression can contribute to human disease including the multistep processes carcinogenesis in human. Circulating miRNAs emerging biomarkers many diseases and cancers such as type 2 diabetes, pulmonary disease, colorectal cancer, gastric cancer among others; however, defining a plasma miRNA signature acute myeloblastic leukemia (AML) that could serve biomarker for...
Cancer development is a complex process that primarily results from the combination of genetic alterations and dysregulation major signalling pathways due to interference with epigenetic machinery. As regulators, miRNAs are central players in control many key tumour factors. These have been classified as oncogenic (oncomiRs) when they target suppressor genes (TS miRNAs) inhibit oncogene protein expression. Most mechanisms modulate oncomiR expression linked transcriptional or...
Regulatory T cells (Tregs) are characterized by a high expression of IL-2 receptor α chain (CD25) and forkhead box P3 (FOXP3), the latter being essential for their development function. Another major player in regulatory function is cytotoxic T-lymphocyte associated molecule-4 (CTLA-4) that inhibits responses. However, regulation CTLA-4 remains less well explored. We therefore studied microRNA signature circulating CD4+ Tregs isolated from adult healthy donors identified composed 15...
Donor lymphocyte infusion has been used in the management of relapsed hematologic malignancies after allogeneic hematopoietic cell transplantation. It can eradicate minimal residual disease or be to rescue a relapse, being able induce durable remissions subset patients. With increased use haploidentical transplantation, there is renewed interest donor lymphocytes either treat prevent relapse post transplant. Published retrospective and small prospective studies have shown encouraging results...
Mesenchymal stromal cells (MSCs) are important in the support of hematopoiesis. In this pilot study, we evaluated safety and efficiency donor-expanded MSC infusion after allogeneic hematopoietic stem cell transplantation (HSCT) six patients with poor recovery. MSCs were infused without HSC conditioning at a dose 1 x 10(6)/kg weight. Two displayed rapid recovery (days 12 21), four showed no response. The two who first complete remission (CR1) compared to other heavily pretreated patients....
microRNAs (miRNAs) are known as potent gene expression regulators, and several studies have revealed the prognostic value of miRNAs in acute myeloid leukemia (AML) patient survival. Recently, strong evidence has indicated that can be transported by exosomes (EXOs) from cancer cells to recipient immune microenvironment (IME) cells.We found AML blast-released EXOs enhance CD3 T-cell apoptosis both CD4 CD8 T cells. We hypothesized present key players mediating changes observed miR-24-3p, a...
Epstein-Barr virus (EBV) reactivation after hematopoietic stem-cell transplantation can lead to posttransplant lymphoproliferative disease (PTLD), which carries a high mortality rate. Among therapeutic and prophylactic options being developed, B-cell depletion with monoclonal antibodies is encouraging. Because viral load correlated PTLD occurrence, we developed preemptive attitude based on polymerase chain reaction (PCR)-guided rituximab administration.We monitored 115 transplant patients...
Few studies thus far have compared head-to-head different non-myelooablative conditioning regimens for allogeneic hematopoietic cell transplantation (allo-HCT). Here, we report the results of a phase II multicenter randomized study comparing non-myeloablative allo-HCT from HLA-identical siblings (n = 54) or 10/10 HLA-matched unrelated donors 40) with either fludarabine plus 2 Gy total body irradiation (Flu-TBI arm; n 49) 8 TLI + anti-thymocyte globulin (TLI-ATG 45) conditioning. The 180-day...
Acute myeloid leukemia (AML) is a cancer of the lineage blood cells, and treatment for AML lengthy can be very expensive. Medicinal plants their bioactive molecules are potential candidates improving human health. In this work, we studied effect Ptychotis verticillata (PV) essential oil its derivatives, carvacrol thymol, in cell lines. We demonstrated that combination thymol induced tumor death with low toxicity on normal cells. Mechanistically, highlighted different molecular pathways,...
Recently, regulatory T (Treg) cells have gained interest in the fields of immunopathology, transplantation and oncoimmunology. Here, we investigated microRNA expression profile human natural CD8+CD25+ Treg impact microRNAs on molecules associated with immune regulation. We purified CD8+ assessed FOXP3 CTLA-4 by flow cytometry. also tested ex vivo suppressive capacity these mixed leukocyte reactions. Using TaqMan low-density arrays qPCR for validation, could identify a 'signature'...