A5091892350- Melanoma and MAPK Pathways
- Cancer-related Molecular Pathways
- Cutaneous Melanoma Detection and Management
- Genetic factors in colorectal cancer
- melanin and skin pigmentation
- Quinazolinone synthesis and applications
- Chronic Lymphocytic Leukemia Research
- Synthesis and biological activity
- Computational Drug Discovery Methods
- MicroRNA in disease regulation
- Cancer Research and Treatments
- Immunotherapy and Immune Responses
- Mesenchymal stem cell research
- PI3K/AKT/mTOR signaling in cancer
- Extracellular vesicles in disease
- Immune cells in cancer
- Cancer, Hypoxia, and Metabolism
- Cancer, Stress, Anesthesia, and Immune Response
- Cell death mechanisms and regulation
- Click Chemistry and Applications
- CAR-T cell therapy research
- Redox biology and oxidative stress
- Mast cells and histamine
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
Institut Jules Bordet
2014-2024
Université Libre de Bruxelles
2014-2024
Institute of Experimental Endocrinology of the Slovak Academy of Sciences
2017
microRNAs (miRNAs) are known as potent gene expression regulators, and several studies have revealed the prognostic value of miRNAs in acute myeloid leukemia (AML) patient survival. Recently, strong evidence has indicated that can be transported by exosomes (EXOs) from cancer cells to recipient immune microenvironment (IME) cells.We found AML blast-released EXOs enhance CD3 T-cell apoptosis both CD4 CD8 T cells. We hypothesized present key players mediating changes observed miR-24-3p, a...
Mitogen-activated protein kinase (MAPK) inhibition with the combination of BRAF (Rapidly Accelerated Fibrosarcoma) and MEK (Mitogen-activated kinase) inhibitors has become standard first-line therapy metastatic melanoma harbouring V600 mutations. However, about half patients present primary resistance while remaining develop secondary under prolonged treatment. Thus, there is a need for predictive biomarkers sensitivity and/or to further refine patient population likely benefit from MAPK...
MEK kinase inhibitors (trametinib and selumetinib) or directed against mutated BRAF(V600E) (vemurafenib dabrafenib) have initial encouraging effects in the treatment of melanoma but acquired resistance appears almost invariably after some months. Studies revealed mutually exclusive NRAS BRAF activating mutations driving MAPK/ERK pathway among human melanomas. Although combination therapy exerts significantly better antitumor cell efficacy, complete remission is rarely achieved. To employ an...
Radiotherapy (RT) in patients with melanoma historically showed suboptimal results, because the disease is often radioresistant due to various mechanisms such as scavenging free radicals by thiols, pigmentary machinery, or enhanced DNA repair. However, radiotherapy has been utilized adjuvant therapy after complete excision of primary and lymph nodes reduce rate nodal recurrences high-risk patients. The resistance cells may also be relation constitutive activation MAPK pathway and/or...
Targeting MAPK pathway in mutant BRAF melanoma with the specific inhibitor vemurafenib showed robust initial responses majority of patients followed by relapses due to acquired resistance drug. In V600EBRAF cell lines, senescence-associated β-galactosidase activity is often encountered a constitutive manner or induced after inhibition. However, link between senescence-like phenotype and inhibition not fully understood yet. Our data validate (low proliferation, high volume, β-Gal activity)...
Treatments of metastatic melanoma underwent an impressive development over the past few years, with emergence small molecule inhibitors targeting mutated proteins, such as BRAF, NRAS, or cKIT. However, since a significant proportion patients acquire resistance to these therapies, new strategies are currently being considered overcome this issue. For purpose, cell lines mutant cKIT and acquired resistances MEK, inhibitors, respectively, were investigated using both 1H-NMR-based metabonomic...
Age-related genetic changes in lymphocyte subsets are not currently well documented. BACH2 is a transcription factor that plays an important role immune-mediated homeostasis by tightly regulating PRDM1 expression both B-cells and T-cells. gene highly sensitive to DNA damage aged mice. This concept led us investigate the variation also major with age. Lymphocyte from 60 healthy donors, 20 90 years, 41 untreated chronic lymphocytic leukemia patients were studied. was analyzed real-time...
Melanoma is the most common cancer in young adults, with a constantly increasing incidence. Metastatic melanoma very aggressive 5-year survival rate of about 22-25%. This is, cases, due to lack therapies which are effective on long term. Hence, it crucial find new therapeutic agents increase patient survival. Toad venoms rich source potentially pharmaceutically active compounds and studies have highlighted their possible effect cells. We focused two different toad species: Bufo bufo Rhinella...
Melanoma is known to be a radioresistant cancer. radioresistance can due several factors such as pigmentation, antioxidant defenses and high Deoxyribonucleic acid (DNA) repair efficacy. However, irradiation induces intracellular translocation of RTKs, including cMet, which regulates response DNA damage activating proteins promotes repair. Accordingly, we hypothesized that co-targeting (PARP-1) relevant activated c-Met in particular, may radiosensitize wild-type B-Raf Proto-Oncogene,...
Potential intrinsic resistance mechanisms to regorafenib were explored after short exposure (3 days) on five CRC cell lines (HCT-116, SW1116, LS-1034, SW480, Caco-2). The observation of senescence-like features led the investigation a drug-initiated phenotype switch. Following long-term (12 months) HCT-116 and SW480 regorafenib, we developed resistant models explore acquired resistance. cells demonstrated senescent-like properties, including arrest in late G2/prophase cycle stage...
The oxygen sensor prolyl hydroxylase domain 2 (PHD2) plays an important role in cell hypoxia adaptation by regulating the stability of HIF proteins (HIF1α and HIF2α) numerous types, including T lymphocytes. on immune cells, particularly regulatory (Treg) function, has not been fully elucidated. purpose our study was to evaluate PHD2 regulation Treg phenotype function. We demonstrate herein that selective ablation expression (PHD2ΔTreg mice) leads a spontaneous systemic inflammatory syndrome,...