A5028194331- Animal Disease Management and Epidemiology
- Immune Cell Function and Interaction
- T-cell and Retrovirus Studies
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- HIV Research and Treatment
- SARS-CoV-2 detection and testing
- Advanced biosensing and bioanalysis techniques
- vaccines and immunoinformatics approaches
- Milk Quality and Mastitis in Dairy Cows
- Reproductive System and Pregnancy
- Bone Tissue Engineering Materials
- Circular RNAs in diseases
- Vector-Borne Animal Diseases
- Cancer-related molecular mechanisms research
- Dental Implant Techniques and Outcomes
- Signaling Pathways in Disease
- Biosensors and Analytical Detection
- Dental materials and restorations
Lebanese University
2014-2024
Institut Jules Bordet
2005-2010
Amis de l'Institut Bordet
2010
Université Libre de Bruxelles
2007-2009
Treg are the main mediators of dominant tolerance. Their mechanisms action and applications subjects considerable debate currently. However, a human microRNA (miR) signature has not been described yet. We investigated natural identified composed five miR (21, 31, 125a, 181c 374). Among those, two were considerably under-expressed (miR-31 miR-125a). functional target sequence for miR-31 in 3' untranslated region (3' UTR) FOXP3 mRNA. Using lentiviral transduction fresh cord blood T cells, we...
Objectives. Hollow space between implant and abutment may act as reservoir for commensal and/or pathogenic bacteria representing a potential source of tissue inflammation. Microbial colonization the interfacial gap ultimately lead to infection bone resorption. Using Rhodamine B, sensitive fluorescent tracer dye, we aim in this study investigate leakage at implant-abutment connection three systems having same prosthetic interface. Materials Methods. Twenty-one implants (seven Astra Tech,...
Abstract The coronavirus disease 2019 (COVID-19) pandemic is unceasingly spreading across the globe, and recently a highly transmissible Omicron SARS-CoV-2 variant (B.1.1.529) has been discovered in South Africa Botswana. Rapid identification of this essential for assessment containment. However, mainly being performed using expensive time-consuming genomic sequencing. In study, we propose an alternative RT-qPCR approach detection BA.1 low-cost rapid SYBR Green method. We have designed...
Growing evidence that the CD3gamma gene is specifically targeted in some T cell diseases focused our attention on need to identify and characterize elusive elements involved transcriptional control. In this study, we show while human CD3delta genes are oriented head-to-head separated by only 1.6 kb, transcribed from an independent but weak, lymphoid-specific TATA-less proximal promoter. Using RNA ligase-mediated rapid amplification of cDNA ends, demonstrate a cluster transcription initiation...
Abstract Background HTLV-I infected CD4 + T-cells lines usually progress towards a CD3 - or low phenotype. In this paper, we studied expression, kinetics, chromatin remodeling of the gene at different time-points post infection. Results The onset phenomenon coincided with decrease γ followed by subsequent progressive reduction in δ, then ε and ζ mRNA. Transient transfection experiments showed that promoter was still active cells demonstrating adequate amounts required transcription factors...
Mature dendritic cells (DCs) represent, by far, the most potent antigen-presenting cells. The development of clinical grade techniques to produce them in large numbers has rendered possible their use trials. It is therefore crucial assess DCs characteristics according methodology used generate them, improve comparison and standardization these We thus compared generated matured culture plates (pla-DCs) or bags (bag-DCs) analyzing, secretion bioactive interleukin (IL)-12 capacity induce...
Abstract The COVID-19 pandemic is unceasingly spreading across the globe, and recently a highly transmissible Omicron SARS-CoV-2 variant (B.1.1.529) has been discovered in South Africa Botswana. Rapid identification of this essential for assessment containment. However, mainly being performed using expensive time-consuming genomic sequencing. In study we propose an alternative RT-qPCR approach detection BA.1 low-cost rapid SYBR Green method. We have designed specific primers to confirm...
Adult T-cell leukemia/lymphoma (ATLL) is a malignancy slowly emerging from human leukemia virus type 1 (HTLV-I)-infected mature CD4þ T-cells. To characterize the molecular modifications induced by HTLV-I infection, we compared HTLVI-infected WE17/10 cells with control cells, using micro-arrays. Many calcium-related genes were progressively downmodulated over period of 2 years. Infected acquired profound decrease intracellular calcium levels in response to ionomycin, timely correlated...
Our studies show that HIV-1, HIV-2, and HTLV-I infection all provoke a progressive defect in surface T cell receptor expression. A specific loss of CD3γ transcripts is responsible for the after HIV-1 or HIV-2 infection. Alternatively, while are lost first infection, their reduction followed several months later by CD3δ subsequently CD3e mRNA. Studies transcriptional control revealed parallels with elements regulating gene expression, including downstream element reminiscent HIV TAR. Mutant...
Our studies show that HIV-1 infection provokes a progressive defect in surface TCR/CD3 receptors due to the specific and escalating loss of CD3 g mRNA. The human gene is transcribed from weak, lymphoid-specific promoter with significant transcription initiation site heterogeneity normal T cells. However, early after transcripts preferentially initiate at +1 +13 further focusing over time as are lost first. Mutant deletion analysis delimited 43 bp sequence critical for positive expression....