A5064038696- Genetics, Aging, and Longevity in Model Organisms
- Cellular transport and secretion
- Circadian rhythm and melatonin
- Biomedical Research and Pathophysiology
- Renin-Angiotensin System Studies
- Pancreatic function and diabetes
- Photosynthetic Processes and Mechanisms
- Phagocytosis and Immune Regulation
- Dialysis and Renal Disease Management
- Ferroptosis and cancer prognosis
- Advanced Fluorescence Microscopy Techniques
- Autophagy in Disease and Therapy
- Vitamin D Research Studies
- Endoplasmic Reticulum Stress and Disease
- Neuroscience and Neuropharmacology Research
- Nitric Oxide and Endothelin Effects
- Effects of Vibration on Health
- Lipid Membrane Structure and Behavior
- Advanced Glycation End Products research
- Selenium in Biological Systems
- Pain Mechanisms and Treatments
- Migraine and Headache Studies
- Nuclear Receptors and Signaling
- Immune Cell Function and Interaction
- Muscle and Compartmental Disorders
University of Würzburg
2015-2024
University of Zanjan
2021
Background: In end-stage renal disease (ESRD), gut-derived uremic toxins play a crucial role in the systemic inflammation and oxidative stress promoting excess morbidity mortality. The biochemical derangement is part consequence of an insufficient generation short-chain fatty acids (SCFA) due to dysbiosis gut consumption fermentable complex carbohydrates. Aim study: primary end-point was evaluate potential efficacy SCFA (specifically, sodium propionate (SP)) for patients on maintenance...
Significance Cells must interact with their environment to survive. The lipids and proteins of the plasma membrane send receive signals at cell surface respond stimuli. When lipid bilayer is damaged, cells release membrane-bound extracellular vesicles repair membrane. also on communicate a distance. Here, we identify that regulate formation from membrane, providing additional tools control can be used test potential functions vesicles. Furthermore, reveal regulating asymmetric localization...
Phagocytic clearance is important to provide cells with metabolites and regulate immune responses, but little known about how phagolysosomes finally resolve their phagocytic cargo of cell corpses, debris, pathogens. While studying the non-apoptotic polar bodies in C. elegans, we previously discovered that tubulate into small vesicles facilitate corpse within 1.5 h. Here, show phagolysosome vesiculation depends on amino acid export by solute transporter SLC-36.1 activation TORC1. We...
Application of electricity for pain treatment dates back to thousands years BC. The Ancient Egyptians and later the Greeks Romans recognized that electrical fishes are capable generating electric shocks relief pain. In 18th 19th centuries these natural producers were replaced by man-made devices. This happened in following phases. first was application static currents (called Franklinism), which produced a friction generator. Christian Kratzenstein apply it medically, followed shortly...
Selenocysteine (Sec) metabolism is crucial for cellular function and ferroptosis prevention begins with the uptake of Sec carrier, selenoprotein P (SELENOP). Following uptake, released from SELENOP metabolized via selenocysteine lyase (SCLY), producing selenide, a substrate selenophosphate synthetase 2 (SEPHS2), which provides essential selenium donor, (H2SePO3-), biosynthesis Sec-tRNA. Here, we discovered an alternative pathway in mediated by peroxiredoxin 6 (PRDX6), independent SCLY....
Epidemiological studies revealed increased renal cancer incidences and higher mortalities in hypertensive individuals. Activation of the renin–angiotensin–aldosterone system leads to formation reactive oxygen species (ROS). In vitro, cells, ex vivo, isolated perfused mouse kidney, we could show DNA-damaging potential angiotensin II (Ang II). Here, pathway involved genotoxicity Ang was investigated. kidney cell lines with properties proximal tubulus an activation NADPH oxidase production ROS,...
ABSTRACT In animals, the midbody coordinates end of cytokinesis when daughter cells separate through abscission. The was thought to be sequestered by macroautophagy, but recent evidence suggests that midbodies are primarily released and phagocytosed. It unknown, however, whether autophagy proteins play a role in phagosome degradation. Using protein degradation assay, we show Caenorhabditis elegans. Released known internalized actin-driven phagocytosis, which requires RAB-5 GTPase localize...
To understand how undifferentiated pluripotent cells cope with cell corpses, we examined the clearance of polar bodies born during female meiosis. We found that lose membrane integrity and expose phosphatidylserine in Caenorhabditis elegans. Polar body signaling recruits engulfment receptors to plasma embryonic blastomeres using PI3K VPS-34, RAB-5 GTPase sorting nexin SNX-6. The second is then phagocytosed receptor-mediated pathways dependent on Rac1 ortholog CED-10 but undergoes...
The cuticle of C. elegans is impermeable to chemicals, toxins, and pathogens. However, increased permeability a desirable phenotype because it facilitates chemical uptake. Surface lipids contribute the barrier. Here, we identify lipid transfer protein GMAP-1 as critical element setting cuticle. A gmap-1 deletion mutant increases cuticular sodium azide, levamisole, Hoechst, DiI. Expressing in hypodermis or transiently adults sufficient rescue this phenotype. secreted from aqueous fluid...
Abstract Visualization of specific organelles in tissues over background fluorescence can be challenging, especially when reporters localize to multiple structures. Instead trying identify proteins enriched membrane-wrapped structures, we use a selective degradation approach remove from the cytoplasm or nucleus C. elegans embryos and mammalian cells. We demonstrate labelling using degron-tagged reporters, including extracellular vesicles, as well individual neighbouring membranes. These...
Objectives . In diabetes accumulated advanced glycation end products (AGEs) are involved in the striking cardiovascular morbidity/mortality. We asked whether a hypovitaminosis D associates with an increased formation and toxicity of AGEs diabetes. Methods 276 diabetics (160 M/116 F, age:<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mn>65.0</mml:mn><mml:mo>±</mml:mo><mml:mn>13.4</mml:mn></mml:math>; 43 type 1,T1DM, 233 2 patients, T2DM) 121 nondiabetic controls (60...
The effect of mild chronic renal failure (CRF) induced by 4/6-nephrectomy (4/6NX) on central neuronal activations was investigated c-Fos immunohistochemistry staining and compared to sham-operated rats. In the 4/6 NX rats also angiotensin receptor blocker, losartan, sympatholyticum moxonidine studied for two months. serial brain sections Fos-immunoreactive neurons were localized classified semiquantitatively. 37 areas/nuclei several with different functional properties strongly affected in...
The neurotransmitter dopamine causes DNA damage, oxidative stress and is involved in the pathology of neurological diseases. To elucidate this potential link we investigated mechanism dopamine-induced damage. We studied role transporter (DAT) MDCK MDCK-DAT cells, containing human DAT gene. After treatment with dopamine, only cells showed elevated chromosomal damage uptake. Although stimulation type 2 receptor (D(2)R) quinpirole absence did not induce genotoxicity rat neuronal PC12...
The lipids phosphatidylserine (PtdSer) and phosphatidylethanolamine (PtdEth) are normally asymmetrically localized to the cytosolic face of membrane bilayers, but can both be externalized during diverse biological processes, including cell division, fusion, death. Externalized in plasma recognized by lipid-binding proteins regulate clearance corpses other debris. However, it is unclear whether PtdSer PtdEth contribute similar or distinct ways these processes. We discovered that disruption...
Selenocysteine (Sec) metabolism is crucial for cellular function and ferroptosis prevention has traditionally been thought to begin with the uptake of Sec carrier selenoprotein P (SELENOP). Following uptake, released from SELENOP undergoes metabolisation via selenocysteine lyase (SCLY), producing selenide, a substrate used by selenophosphate synthetase 2 (SEPHS2), which provides essential selenium donor - biosynthesis tRNA. Here, we report discovery an alternative pathway mediating that...
The aim of this study was to assess potential effects high-tone external muscle stimulation (HTEMS) on parameters endothelial dysfunction (ED) in patients with acute kidney injury (AKI).The bad outcome AKI is markedly influenced by ED, microinflammation, oxidative stress and protein hypercatabolism. Recently, we have shown that intradialytic application HTMS associated a faster resolution AKI. Here, investigated the same cohort whether ED such as nitric oxide (NO),...
The prognosis of acute kidney injury (AKI) is markedly influenced by the degree muscle protein catabolism. Since current therapeutic strategies are rather limited, for first time, we attempted to attenuate hypercatabolism high tone electrical stimulation (HTEMS) in AKI patients. This kind therapy may lower degradation via its effect on activity as well improving insulin resistance. Moreover, electrotherapy improve renal function due circulatory effects lowering sympathetic tone.34 patients...
This research investigated the bearing capacity and geotechnical properties of a sandy soil substrate contaminated with oil derivatives diesel fuel kerosene. For this purpose, site clayey was considered to evaluate effects contamination on in both clean states. Then, artificially kerosene underwent field laboratory tests. The experiments, including Atterberg limits, standard Proctor compaction, uniaxial compressive strength, strength freeze–thaw durability tests, were performed prepared...