A5019341580- Environmental Toxicology and Ecotoxicology
- Heavy Metal Exposure and Toxicity
- Mercury impact and mitigation studies
- Trace Elements in Health
- Heavy metals in environment
- Toxic Organic Pollutants Impact
- Nanoparticles: synthesis and applications
- Air Quality and Health Impacts
- Carcinogens and Genotoxicity Assessment
- Fungal and yeast genetics research
- Bacterial Genetics and Biotechnology
- Effects and risks of endocrine disrupting chemicals
- Lipid Membrane Structure and Behavior
- Fermentation and Sensory Analysis
- Electrochemical Analysis and Applications
- Radioactive contamination and transfer
- Drug Transport and Resistance Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Heat shock proteins research
- Aquatic Invertebrate Ecology and Behavior
- Vibrio bacteria research studies
- Weed Control and Herbicide Applications
- Pharmaceutical and Antibiotic Environmental Impacts
- Marine Biology and Environmental Chemistry
- Cellular transport and secretion
Centre National de la Recherche Scientifique
2015-2024
Université de Bordeaux
2015-2024
Institut Européen de Chimie et Biologie
2017-2024
Environnements et Paléoenvironnements Océaniques et Continentaux
2008-2019
Institut de Biochimie et Génétique Cellulaires
1996-2010
Institut de Radioprotection et de Sûreté Nucléaire
2010
General Engineering Measurement and Automation (Czechia)
2008
Écologie Fonctionnelle et Écotoxicologie des Agroécosystèmes
2006
University of Groningen
2005
Laboratoire des Interactions Plantes Micro-Organismes
1995
Effects of dietary methylmercury (MeHg) on gene expression were examined in three organs (liver, skeletal muscle, and brain) the zebrafish (Danio rerio). Adult male fish fed over 7, 21, 63 days different diets: one control diet (C0: 0.08 μg Hg g-1, dry wt) two diets (C1 C2) contaminated by MeHg at 5 13.5 wt. Total concentrations determined after each exposure duration, a demethylation process was evidenced only liver. Thirteen genes known to be involved antioxidant defenses, metal chelation,...
Toxicity of methylmercury (MeHg) to wildlife and humans results from its binding cysteine residues proteins, forming MeHg-cysteinate (MeHgCys) complexes that hinder biological functions. MeHgCys can be detoxified in vivo, yet how this occurs is unknown. We report are transformed into selenocysteinate [Hg(Sec)4] multiple animals two phyla (a waterbird, freshwater fish, earthworms) sampled different geographical areas contaminated by Hg sources. In addition, high energy-resolution X-ray...
The impact of a daily ration food containing gold nanoparticles (AuNPs) two sizes (12 and 50 nm) was investigated in the zebrafish Danio rerio at very low doses (from 36-106 ng gold/fish/day). AuNP exposure resulted various dysfunctions sub cellular scale, concentration food, size duration modulated observed adverse effects. Indeed, we showed alteration genome composition using RAPD-PCR genotoxicity test as number hybridization sites RAPD probes significantly modified after exposure....
Increasing use of metallic nanomaterials is likely to result in release these particles into aquatic environments; nevertheless it unclear whether materials present a hazard organisms. The impact contaminated sediment containing 14-nm gold nanoparticles (AuNPs) was investigated the zebrafish Danio rerio exposed for 20 days two concentrations, 16 and 55 µg/g dry weight. AuNPs were released from water column, during this period mean concentrations AuNP filtered fraction 0.25 ± 0.05 0.8 0.1...
The kinetics of K+ efflux caused by colicin A in Escherichia coli-sensitive cells have been investigated using a K(+)-selective electrode. order magnitude the rate per molecule was comparable to that ion channels. dependence upon multiplicity, pH, temperature, and membrane potential (delta psi) determined. translocation from outer receptor inner insertion into required fluid membrane, but once inserted, channel properties showed little state lipids. At given lag time before onset found...
Abstract The effects of waterborne uranium (U) exposure on gene expression were examined in four organs (brain, liver, skeletal muscles, and gills) the zebrafish (Danio rerio). Adult male fish exposed to three treatments: No added (control), 23 ± 6 μg U/L, 130 34 U/L. After 3, 10, 21, 28 d an 8-d depuration period, bioaccumulation analyzed. Bioaccumulation decreased significantly liver during phase, genes involved detoxification, apoptotic mechanism, immune response strongly induced. Among...
ABSTRACT The wine bacterium Oenococcus oeni has to cope with harsh environmental conditions, including an acidic pH, a high alcoholic content, nonoptimal growth temperatures, and growth-inhibitory compounds such as fatty acids, phenolic tannins. We describe the characterization cloning of O. ftsH gene, encoding protease belonging ATP binding cassette protein superfamily. FtsH is closest in sequence similarity homologue Lactococcus lactis . gene proved be stress-responsive, since its...
To address the impact of cadmium sulphide nanoparticles (CdSNPs) two different sizes (8 and 50 nm), Danio rerio zebrafish were dietary exposed to very low doses: 100 or 40 ng CdSNPs/day/g body weight for 36 60 days, respectively. The results obtained using RAPD-PCR genotoxicity test showed genomic alteration since number hybridisation sites RAPD probes was significantly modified after CdSNPs exposure. In addition, selected stress response genes either repressed upregulated in tissues...
The freshwater cyprinid Tanichthys albonubes was used to assess the bioavailability of divalent mercury (Hg(II)) complexed in dissolved organic matter (DOM) fish. fish acquired 0.3 2.2 μg Hg/g dry weight after 8 weeks aquaria containing DOM from a Carex peat with at initial concentrations 14 nM 724 nM. Changes relative proportions dithiolate Hg(SR)2 and nanoparticulate β-HgS DOM, as quantified by high energy-resolution XANES (HR-XANES) spectroscopy, indicate that complexes either produced...
To address and to compare the respective impact of gold silver nanoparticles (Au Ag NPs) in soil invertebrate, earthworm Eisenia fetida was exposed containing 2, 10, 50 mg/kg Au both nanoparticulate ionic forms for 10 days. Both metal NPs were 2–15 times less bioavailable than their forms, displayed similar transfer coefficients from tissues. triggered onset an oxidative stress as illustrated by increased glutathione S-transferase levels, decreased catalase malondialdehyde concentrations....
GPI anchors are widely represented among organisms and have several cellular functions. It has been proposed that in yeast there two groups of proteins: plasma membrane-resident proteins, such as Gas1p or Yap3p, cell wall-targeted Tir1p alpha-agglutinin. A model for the membrane retention proteins from first group because a dibasic motif located just upstream GPI-anchoring signal. The results we report here not agreement with show constructs containing C-terminal parts Yap3p also targeted to...
Mutations in tolQ, previously designated fii, render cells tolerant to high concentrations of colicin A. In addition, a short deletion the amino-terminal region A (amino acid residues 16 29) prevents its lethal action, although this protein can still bind receptor and forms channels planar lipid bilayers vitro. These defects translocation across outer membrane tolQ or mutant cannot be bypassed by osmotic shock. The TolQ protein, which is constitutively expressed at low level, was studied...
Mercury (Hg) is a widespread environmental contaminant and its organic form, methylmercury (MeHg), has been known as potent neurotoxic since the Minamata tragedy. In Amazonian basin, gold mining leads to MeHg biomagnification all along food web, culminating in piscivorous fish, ultimately responsible for contamination of human beings through fish consumption. order assess biological impact dietary on at genome scale, we contaminated zebrafish with MeHg-contaminated 25 days (13.5 μg Hg/g...