A5086163487- Neuroblastoma Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Cancer therapeutics and mechanisms
- Glioma Diagnosis and Treatment
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Synthesis and biological activity
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Adipose Tissue and Metabolism
- Cancer Genomics and Diagnostics
- Bioactive Compounds and Antitumor Agents
- Renal and related cancers
- Aquaculture disease management and microbiota
- Monoclonal and Polyclonal Antibodies Research
- Eicosanoids and Hypertension Pharmacology
- Cell Adhesion Molecules Research
- Cytokine Signaling Pathways and Interactions
- Cardiac Ischemia and Reperfusion
- Lung Cancer Research Studies
- Protein Degradation and Inhibitors
- Telomeres, Telomerase, and Senescence
- Cancer-related Molecular Pathways
- Muscle Physiology and Disorders
- Immune cells in cancer
Cancer Research Institute Ghent
2017-2024
Ghent University
2015-2024
Ghent University Hospital
2016-2024
High-risk neuroblastoma, a pediatric tumor originating from the sympathetic nervous system, has low mutation load but highly recurrent somatic DNA copy number variants. Previously, segmental gains and/or amplifications allowed identification of drivers for neuroblastoma development. Using this approach, combined with gene dosage impact on expression and survival, we identified ribonucleotide reductase subunit M2 (RRM2) as candidate dependency factor further supported by growth inhibition...
Abstract Pediatric central nervous system tumors remain challenging to diagnose. Imaging approaches do not provide sufficient detail discriminate between different tumor types, while the histopathological examination of tissue shows high inter-observer variability. Recent studies have demonstrated accurate classification based on DNA methylation profile a biopsy. However, brain biopsy holds significant risk bleeding and damaging surrounding tissues. Liquid analyzing circulating show...
Abstract Neuroblastoma is an aggressive childhood cancer arising from sympatho-adrenergic neuronal progenitors. The low survival rates for high-risk disease point to urgent need novel targeted therapeutic approaches. Detailed molecular characterization of the neuroblastoma genomic landscape indicates that ALK- activating mutations are present in 10% primary tumours. Together with other causing RAS/MAPK pathway activation, ALK also enriched relapsed cases and activation was shown accelerate...
Abstract The ongoing ascent of sequencing technologies has enabled researchers to gain unprecedented insights into the RNA content biological samples. MiRNAs, a class small non-coding RNAs, play pivotal role in regulating gene expression. discovery that miRNAs are stably present circulation spiked interest their potential use as minimally-invasive biomarkers. However, blood-derived samples (serum, plasma) is challenging due often low concentration, poor quality and presence highly abundant...
Effective inhibitors of invasion and metastasis represent a serious unmet clinical need. We have recently identified 4-fluoro-3',4',5'-trimethoxychalcone or C16 as potent anti-invasive molecule. In this paper, we report on the development an optimized vehicle for oral administration C16. also explore its pharmacokinetic toxicity profile in rodents prelude to broad-scope evaluation pharmacological tool animal models disease. showed suboptimal pharmacokinetics with limited bioavailability...
Abstract MYCN is an oncogenic driver in neural crest-derived neuroblastoma and medulloblastoma. To better understand the early effects of activation a neural-crest lineage context, we profiled transcriptome immortalized human retina pigment epithelial cells with inducible activation. Gene signatures associated elevated MYC/MYCN activity were induced after 24 h activation, which attenuated but sustained at later time points. Unexpectedly, was accompanied by reduced cell growth. set enrichment...
Human embryonic stem cells (hESCs) and embryonal tumors share a number of common features, including compromised G1/S checkpoint. Consequently, these rapidly dividing hESCs cancer undergo elevated levels replicative stress, inducing genomic instability that drives chromosomal imbalances. In this context, it is interest long-term in vitro cultured exhibit remarkable high incidence segmental DNA copy gains, some which are also highly recurrent certain malignancies such as 17q gain (17q+). The...
The goal of the chick heart assay is to offer a relevant organ culture method study tumor invasion in three dimensions. can distinguish between invasive and non-invasive cells, enables effects test compounds on invasion. Cancer cells - either as aggregates or single are confronted with fragments embryonic heart. After suspension for few days weeks confronting cultures fixed embedded paraffin histological analysis. three-dimensional interaction cancer normal tissue then reconstructed from...
Summary Neuroblastoma is a pediatric tumor originating from the sympathetic nervous system responsible for 10-15 percent of all childhood cancer deaths. Half neuroblastoma patients present with high-risk disease at diagnosis. Despite intensive multi-modal therapies nearly 50 cases relapse and die their disease. In contrast to overall paucity mutations, invariably recurrent somatic segmental chromosome copy number variants. For several focal aberrations ( e.g. MYCN LIN28B amplification),...
The goal of the chick heart assay is to offer a relevant organ culture method study tumor invasion in three dimensions. can distinguish between invasive and non-invasive cells, enables effects test compounds on invasion. Cancer cells - either as aggregates or single are confronted with fragments embryonic heart. After suspension for few days weeks confronting cultures fixed embedded paraffin histological analysis. three-dimensional interaction cancer normal tissue then reconstructed from...
Abstract Pediatric central nervous system tumors remain challenging to diagnose. Imaging approaches do not provide sufficient detail discriminate between different tumor types, while the histopathological examination of tissue shows high interobserver variability. Recent studies have demonstrated accurate classification based on DNA-methylation profile a biopsy. However, brain biopsy holds significant risk bleeding and damaging surrounding tissues. Liquid analyzing circulating DNA show...