A5070359037- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neural Engineering
- RNA modifications and cancer
- Neurogenesis and neuroplasticity mechanisms
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- Immunodeficiency and Autoimmune Disorders
- Digestive system and related health
- Bacterial Genetics and Biotechnology
- Pluripotent Stem Cells Research
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Genomics and Chromatin Dynamics
- Medical Imaging and Pathology Studies
- Bacteriophages and microbial interactions
- Cytomegalovirus and herpesvirus research
- Single-cell and spatial transcriptomics
- Cell Adhesion Molecules Research
- Cancer-related gene regulation
- Metallurgy and Material Science
- Animal Genetics and Reproduction
- Salivary Gland Tumors Diagnosis and Treatment
Ludwig-Maximilians-Universität München
2018-2022
Institute of Bioinformatics and Systems Biology
2022
Helmholtz Zentrum München
2016-2020
Bernstein Center for Computational Neuroscience Munich
2018-2020
Center for Environmental Health
2018
Center for Neurosciences
2018
Abstract Master transcription factors have the ability to direct and reverse cellular identities, consequently their genes must be subject particular transcriptional control. However, it is unclear which molecular processes are responsible for impeding activation safeguarding identities. Here we show that targeting of dCas9-VP64 promoter master factor Sox1 results in strong transcript protein up-regulation neural progenitor cells (NPCs). This gene restores lost neuronal differentiation...
Astrocyte-to-neuron conversion is a promising avenue for neuronal replacement therapy. Neurons are particularly dependent on mitochondrial function, but how well mitochondria adapt to the new fate unknown. Here, we determined comprehensive proteome of cortical astrocytes and neurons, identifying about 150 significantly enriched proteins each cell type, including transporters, metabolic enzymes, cell-type-specific antioxidants. Monitoring their transition during reprogramming revealed late...
Novel applications based on the bacterial CRISPR system make genetic, genomic, transcriptional and epigenomic engineering widely accessible for first time. A significant advantage of over previous methods is its tremendous adaptability due to bipartite nature. Cas9 or engineered variants define molecular effect, while short gRNAs determine targeting sites. majority approaches depend simultaneous delivery multiple into single cells, either as an essential precondition, increase responsive...
Zebrafish have a high capacity to replace lost neurons after brain injury. New involved in repair are generated by specific set of glial cells, known as ependymoglial cells. We analyze changes the transcriptome cells and their progeny injury infer molecular pathways governing restorative neurogenesis. identify aryl hydrocarbon receptor (AhR) regulator ependymoglia differentiation toward post-mitotic neurons. In vivo imaging shows that AhR signaling promotes direct conversion subset into...
The bacterial CRISPR system is fast becoming the most popular genetic and epigenetic engineering tool due to its universal applicability adaptability. desire deploy CRISPR-based methods in a large variety of species contexts has created an urgent need for development easy, time- cost-effective enabling large-scale screening approaches. Here we describe CORALINA (comprehensive gRNA library generation through controlled nuclease activity), method comprehensive libraries screens. can be derived...
(Cell Stem Cell 28, 524–534.e1–e7, March 4, 2021) In the originally published version of our manuscript, values in Table S1 were missing a decimal separator (dot) and some columns moved due to program incompatibilities when transferring original data from .csv .xlsx. To address this, we have now added “.” corrected so that Excel file corresponds file. We apologize for oversight any resulting confusion. CRISPR-Mediated Induction Neuron-Enriched Mitochondrial Proteins Boosts Direct...
The oligodendrocyte progenitors (OPCs) are at the front of glial reaction to traumatic brain injury. However, regulatory pathways steering OPC as well role reactive OPCs remain largely unknown. Here, we compared a long-lasting, exacerbated adult zebrafish injury with timely restricted activation identify specific molecular mechanisms regulating reactivity and their contribution regeneration. We demonstrated that influx cerebrospinal fluid into parenchyma after simultaneously activates...
Abstract The CRISPR/Cas9 system has emerged as a promising platform for gene editing; however, the lack of an efficient and safe delivery to introduce it into cells continues hinder clinical translation. Here, we report rationally designed gene‐editing nanoparticle (NP) formulation brain applications: sgRNA:Cas9 ribonucleoprotein complex is immobilized on NP surface by oligonucleotides that are complementary sgRNA. Irradiation with near‐infrared (NIR) laser generates heat in NP, leading...
Abstract The CRISPR/Cas9 system has emerged as a promising platform for gene editing; however, the lack of an efficient and safe delivery to introduce it into cells continues hinder clinical translation. Here, we report rationally designed gene‐editing nanoparticle (NP) formulation brain applications: sgRNA:Cas9 ribonucleoprotein complex is immobilized on NP surface by oligonucleotides that are complementary sgRNA. Irradiation with near‐infrared (NIR) laser generates heat in NP, leading...
Nanobodies are emerging as critical tools for drug design. Several have been recently created to serve inhibitors of severe acute respiratory syndrome coronavirus s (SARS-CoV-2) entry in the host cell by targeting surface-exposed spike protein. Here we established a pipeline that instead targets highly conserved viral proteins made only after into when SARS-CoV-2 RNA-based genome is translated. As proof principle, designed nanobodies against non-structural protein (Nsp)9, which required...
Abstract Nanobodies are emerging as critical tools for drug design. Several have been recently created to serve inhibitors of SARS-Cov-2 entry in the host cell by targeting surface-exposed Spike protein. However, due high frequency mutations that affect Spike, these nanobodies may not target it their full potential and a consequence, inhibition viral be efficient. Here we established pipeline instead targets highly conserved proteins made only after into when RNA-based genome is translated....
The bacterial CRISPR/Cas9 system has substantially increased methodological options for life scientists. Due to its utilization, genetic and genomic engineering became applicable a large range of systems. Moreover, many transcriptional epigenomic approaches are now generally feasible the first time. One reason broad applicability CRISPR lies in bipartite nature. Small gRNAs determine targets complex, variants protein Cas9, local molecular consequences. However, depend on simultaneous...
The bacterial CRISPR/Cas9 system has substantially increased methodological options for life scientists. Due to its utilization, genetic and genomic engineering became applicable a large range of systems. Moreover, many transcriptional epigenomic approaches are now generally feasible the first time. One reason broad applicability CRISPR lies in bipartite nature. Small gRNAs determine targets complex, variants protein Cas9, local molecular consequences. However, depend on simultaneous...