A5011359129- Nanoparticle-Based Drug Delivery
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- Neuroscience and Neural Engineering
- Advanced Nanomaterials in Catalysis
- Glioma Diagnosis and Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- CRISPR and Genetic Engineering
- Barrier Structure and Function Studies
- Brain Metastases and Treatment
- Lung Cancer Research Studies
- Graphene and Nanomaterials Applications
- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
University of Coimbra
2021-2025
Centro de Neurociências e Biologia Celular
2024
Center for Neurosciences
2024
Polytechnic University of Turin
2019
Brain metastasis (BrM) is a devastating end-stage neurological complication that occurs in up to 50% of human epidermal growth factor receptor 2-positive (HER2+) breast cancer (BC) patients. Understanding how disseminating tumor cells manage cross the blood-brain barrier (BBB) essential for developing effective preventive strategies. We identified ecto-nucleotidase ENPP1 (ectonucleotide pyrophosphatase/phosphodiesterase 1) as specifically enriched secretome HER2+ brain metastatic cells,...
Abstract In this study, hybrid nanocubes composed of magnetite (Fe 3 O 4 ) and manganese dioxide (MnO 2 ), coated with U‐251 MG cell‐derived membranes (CM‐NCubes) are synthesized. The CM‐NCubes demonstrate a concentration‐dependent oxygen generation (up to 15%), and, for the first time in literature, an intracellular increase temperature (6 °C) due exothermic scavenging reaction hydrogen peroxide (H is showed. Internalization studies that internalized much faster at higher extent by...
The development of effective neurological treatments remains a challenge due to the low permeation across blood-brain barrier (BBB). use nanoparticles for brain drug delivery is promising, but level BBB crossing low, typically around 0.5-3%. We report synthesis negatively charged N-isopropylacrylamide nanogels (diameter 10 nm) displaying in vitro human model nearly 5-fold higher than previously reported, and permeating parenchyma healthy zebrafish with fully formed BBB....
The development of effective neurological treatments remains a challenge due to the low permeation across blood-brain barrier (BBB). use nanoparticles for brain drug delivery is promising, but level BBB crossing low, typically around 0.5-3%. We report synthesis negatively charged N-isopropylacrylamide nanogels (diameter 10 nm) displaying in vitro human model nearly 5-fold higher than previously reported, and permeating parenchyma healthy zebrafish with fully formed BBB....
Abstract Ischemic stroke is considered one of the most threatening neurological disorders with high percentages mortality and morbidity worldwide. Although in recent years, several nanotechnological advances have improved survival rates, severe untreated poststroke side‐effects continue to significantly influence way life many. Tissue plasminogen activator mechanical thrombectomy are gold standards for treatment acute cerebral ischemia. These, however, fail improve postischemic disorders....
Abstract The CRISPR/Cas9 system has emerged as a promising platform for gene editing; however, the lack of an efficient and safe delivery to introduce it into cells continues hinder clinical translation. Here, we report rationally designed gene‐editing nanoparticle (NP) formulation brain applications: sgRNA:Cas9 ribonucleoprotein complex is immobilized on NP surface by oligonucleotides that are complementary sgRNA. Irradiation with near‐infrared (NIR) laser generates heat in NP, leading...
<title>Abstract</title> Background Drug delivery to the brain is challenging due restrict permeability of blood barrier (BBB). Recent studies indicate that BBB increases over time during physiological aging likely factors (including extracellular vesicles (EVs)) exist in bloodstream. Therefore, inspiration can be taken from develop new strategies for transient opening drug brain. Results Here, we evaluated impact small EVs (sEVs) enriched with microRNAs (miRNAs) overexpressed aging, capacity...
Abstract The CRISPR/Cas9 system has emerged as a promising platform for gene editing; however, the lack of an efficient and safe delivery to introduce it into cells continues hinder clinical translation. Here, we report rationally designed gene‐editing nanoparticle (NP) formulation brain applications: sgRNA:Cas9 ribonucleoprotein complex is immobilized on NP surface by oligonucleotides that are complementary sgRNA. Irradiation with near‐infrared (NIR) laser generates heat in NP, leading...