A5067622760- Extracellular vesicles in disease
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Barrier Structure and Function Studies
- Graphene and Nanomaterials Applications
- Tissue Engineering and Regenerative Medicine
- RNA regulation and disease
- Mesenchymal stem cell research
University of Coimbra
2022-2024
Centro de Neurociências e Biologia Celular
2024
Parkinson's disease is a neurodegenerative characterized by the loss of dopaminergic neurons in substantia nigra with no effective cure available. MicroRNA-124 has been regarded as promising therapeutic entity for due to its pro-neurogenic and neuroprotective roles. However, efficient delivery brain remains challenging. Here, we used umbilical cord blood mononuclear cell-derived extracellular vesicles biological vehicle deliver microRNA (miR)-124-3p evaluate effects mouse model disease. In...
Endothelial cell (EC) activity is essential for tissue regeneration in several (patho)physiological contexts. However, our capacity to deliver vivo biomolecules capable of controlling EC fate relatively limited. Here, we screened a library microRNA (miR) mimics and identified 25 miRs enhancing the survival ECs exposed ischemia-mimicking conditions. In vitro, showed that miR-425-5p, one hits, was able enhance migration. vivo, using mouse Matrigel plug assay, transfected with miR-425-5p...
Therapeutic cells are usually administered as living agents, despite the risks of undesired cell migration and acquisition unpredictable phenotypes. Additionally, most cell-based therapies rely on administration single cells, often associated with rapid in vivo clearance. 3D cellular materials may be useful to prolong effect offer possibility creating structural volumetric constructs. Here, manufacturing shape-versatile fixed immunomodulatory properties is reported. Living aggregates...
<title>Abstract</title> Background Drug delivery to the brain is challenging due restrict permeability of blood barrier (BBB). Recent studies indicate that BBB increases over time during physiological aging likely factors (including extracellular vesicles (EVs)) exist in bloodstream. Therefore, inspiration can be taken from develop new strategies for transient opening drug brain. Results Here, we evaluated impact small EVs (sEVs) enriched with microRNAs (miRNAs) overexpressed aging, capacity...