A5042047372- Osteoarthritis Treatment and Mechanisms
- Metabolism, Diabetes, and Cancer
- Barrier Structure and Function Studies
- Pluripotent Stem Cells Research
- Inflammatory mediators and NSAID effects
- Angiogenesis and VEGF in Cancer
- Extracellular vesicles in disease
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Peroxisome Proliferator-Activated Receptors
- Retinal Development and Disorders
- Natural product bioactivities and synthesis
- Immunotherapy and Immune Responses
- Neuroscience and Neural Engineering
- Cancer, Hypoxia, and Metabolism
- Adipose Tissue and Metabolism
- High Altitude and Hypoxia
- MicroRNA in disease regulation
- Congenital heart defects research
- Neuroinflammation and Neurodegeneration Mechanisms
- Nuclear Structure and Function
- Sesquiterpenes and Asteraceae Studies
- Virus-based gene therapy research
- Seed and Plant Biochemistry
University of Coimbra
2008-2025
Centro de Neurociências e Biologia Celular
2004-2024
Hospital Israelita Albert Einstein
2022
John Wiley & Sons (United States)
2019
Hudson Institute
2019
Center for Neurosciences
2006-2008
Nagasaki University
2008
Cell2B (Portugal)
2005
Abstract Introduction Disorders that affect glucose metabolism, namely diabetes mellitus (DM), may favor the development and/or progression of osteoarthritis (OA). Thus far, little is known regarding ability chondrocytes to adjust variations in extracellular concentration, resulting from hypoglycemia and hyperglycemia episodes, so, avoid deleterious effects deprivation or intracellular accumulation glucose. The aim this study was compare normal OA regulate their transport capacity conditions...
Cartilage matrix homeostasis involves a dynamic balance between numerous signals that modulate chondrocyte functions. This study aimed at elucidating the role of extracellular glucose concentration in modulating anabolic and catabolic gene expression normal osteoarthritic (OA) human chondrocytes its ability to modify responses induced by pro-anabolic stimuli, namely Transforming Growth Factor-β (TGF). For this, we analyzed real time RT-PCR articular cartilage matrix-specific non-specific...
The use of engineered cardiac tissue for high-throughput drug screening/toxicology assessment remains largely unexplored. Here we propose a scaffold that mimics aspects extracellular matrix while preserving the contractility cardiomyocytes. is based on poly(caprolactone) (PCL) nanofilm with magnetic properties (MNF, standing nanofilm) coated layer piezoelectric (PIEZO) microfibers poly(vinylidene fluoride–trifluoroethylene) (MNF+PIEZO). creates flexible support cell contraction and aligned...
Abstract The current work reports the functional characterization of human induced pluripotent stem cells (iPSCs)- arterial and venous-like endothelial (ECs), derived in chemically defined conditions, either monoculture or seeded a scaffold with mechanical properties similar to blood vessels. iPSC-derived arterial- were obtained two steps: differentiation iPSCs into precursor (CD31 pos /KDR /VE-Cad med /EphB2 neg /COUP-TF ) followed by their ECs using high low vascular growth factor (VEGF)...
The derivation of human brain capillary endothelial cells is utmost importance for drug discovery programs focusing on diseases the central nervous system. Here, we describe a two-step differentiation protocol to derive capillary-like from pluripotent stem cells. were initially differentiated into progenitor followed by specification cell phenotype using that combined induction, in time-dependent manner, VEGF, Wnt3a, and retinoic acid signaling pathways use fibronectin as extracellular...
Disease tolerance is a defensive strategy that limits tissue damage during infection. Macrophage migration inhibitory factor (MIF)-deficient mice ( Mif-/- ) are protected in different models of infection and intestinal inflammation due to unclear disease mechanisms, whereas low disease-tolerant Il10-/- develop microbiota-dependent spontaneous gut inflammation. Here, we examined whether IL-10 required for the phenotype seen and, conversely, contribution MIF deficiency. While breeding...
Abstract ATP‐sensitive potassium [K(ATP)] channels sense intracellular ATP/ADP levels, being essential components of a glucose‐sensing apparatus in various cells that couples glucose metabolism, levels and membrane potential. These are present human chondrocytes, but their subunit composition functions unknown. This study aimed at elucidating the K(ATP) expressed chondrocytes determining whether they play role regulating abundance major transporters, GLUT‐1 GLUT‐3, transport capacity. The...
Birth defects, which are in part caused by exposure to environmental chemicals and pharmaceutical drugs, affect 1 every 33 babies born the United States each year. The current standard screen drugs that embryonic development is based on prenatal animal testing; however, this approach yields low-throughput limited mechanistic information regarding biological pathways potential adverse consequences humans. To develop a screening platform for molecules human endothelial cells (ECs) derived from...
More effective approaches are needed in the treatment of blood cancers, particular acute myeloid leukemia (AML), that able to eliminate resistant stem cells (LSCs) at bone marrow (BM), after a chemotherapy session, and then enhance hematopoietic cell (HSC) engraftment for re-establishment HSC compartment. Here, we investigate whether light-activatable nanoparticles (NPs) encapsulating all-trans-retinoic acid (RA+NPs) could solve both problems. Our vitro results show mouse AML transfected...
BBB dysfunction during aging is characterized by an increase in its permeability and phenotypic alterations of brain endothelial cells (BECs) including dysregulation tight junction's expression. Here we have investigated the role BEC senescence BBB. Our results suggest that transition from young to aged mediated, at least part senescence.
purpose. Altered glutamatergic neurotransmission and calcium homeostasis may contribute to retinal neural cell dysfunction apoptosis in diabetic retinopathy (DR). The purpose of this study was determine the effect high glucose on protein content α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) kainate glutamate receptor subunits, particularly GluR2 subunit, because it controls Ca2+ permeability AMPA receptor-associated channels. concentration cytosolic free ([Ca2+]i) also...
Nuclear factor-κB is a key transcription factor activated by pro-inflammatory signals, like interleukin-1β (IL-1), being required for the expression of many inflammatory and catabolic mediators, such as nitric oxide (NO), that play an important role in arthritic diseases. This work aimed at screening identifying natural inhibitors IL-induced NF-κB activation NO production human articular chondrocytes. Five essential oils obtained from four plants Iberian flora, Mentha x piperita L....
Abstract In vitro cell‐based models that better mimic the human heart tissue are of utmost importance for drug development and cardiotoxicity testing but also as tools to understand mechanisms related with disease at cellular molecular level. Besides, implementation analytical allow depiction comprehensive understanding crosstalk between different cell types is relevant. this work, we implemented a cardiac tissue‐like in model, derived from human‐induced pluripotent stem (hiPSC), evaluated...
The sensitizing potential of chemicals is currently assessed using animal models. However, ethical and economic concerns the recent European legislative framework triggered intensive research efforts in development validation alternative methods. Therefore, aim this study was to develop an vitro predictive test based on analysis integration gene expression intracellular signaling profiles chemical-exposed skin-derived dendritic cells. Cells were treated with four known sensitizers two...
Recent epidemiologic and experimental data reinforced the concept that diabetes mellitus (DM) is an independent risk factor for osteoarthritis (OA). Besides a systemic inflammatory response can affect joint tissues contribute to OA pathogenesis, direct effects of hyperglycaemia have been shown cause cell damage induce inflammation by various mechanisms in several associated diabetic complications. Whether how glucose directly affects cells just beginning be unraveled. Indirect high result...
<title>Abstract</title> Background Drug delivery to the brain is challenging due restrict permeability of blood barrier (BBB). Recent studies indicate that BBB increases over time during physiological aging likely factors (including extracellular vesicles (EVs)) exist in bloodstream. Therefore, inspiration can be taken from develop new strategies for transient opening drug brain. Results Here, we evaluated impact small EVs (sEVs) enriched with microRNAs (miRNAs) overexpressed aging, capacity...