A5010296434- Tissue Engineering and Regenerative Medicine
- Cardiac Fibrosis and Remodeling
- Electrospun Nanofibers in Biomedical Applications
- Mesenchymal stem cell research
- Congenital heart defects research
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neural Engineering
- Cardiomyopathy and Myosin Studies
- Cardiac Structural Anomalies and Repair
- Pluripotent Stem Cells Research
- Cardiac Ischemia and Reperfusion
- Cardiovascular Function and Risk Factors
- Elasticity and Material Modeling
- Circular RNAs in diseases
- 3D Printing in Biomedical Research
- Mechanical Circulatory Support Devices
- Cardiovascular Effects of Exercise
- CRISPR and Genetic Engineering
- Advanced Sensor and Energy Harvesting Materials
- Cellular and Composite Structures
- Pancreatic function and diabetes
- RNA modifications and cancer
- RNA regulation and disease
- Protease and Inhibitor Mechanisms
- Mitochondrial Function and Pathology
Imperial College London
2015-2025
Medizinische Hochschule Hannover
2019-2024
Sana Biotechnology (United States)
2023
Hammersmith Hospital
2019
University of Oxford
2012-2016
University of Verona
2012-2016
Semmelweis University
2016
King's College London
2016
Hospital General Universitario Gregorio Marañón
2016
Tampere University
2016
An auxetic conductive cardiac patch (AuxCP) for the treatment of myocardial infarction (MI) is introduced. The design gives a negative Poisson's ratio, providing it with ability to conform demanding mechanics heart. conductivity allows interface electroresponsive tissues such as Excimer laser microablation used micropattern re-entrant honeycomb (bow-tie) into chitosan-polyaniline composite. It shown that bow-tie can produce patches wide range in mechanical strength and anisotropy, which be...
Electrically active constructs can have a beneficial effect on electroresponsive tissues, such as the brain, heart, and nervous system. Conducting polymers (CPs) are being considered components of these because their intrinsic electroactive flexible nature. However, clinical application has been largely hampered by short operational time due to decrease in electronic properties. We show that, immobilizing dopant conductive scaffold, we prevent its electric deterioration. grew polyaniline...
The EMPA-REG OUTCOME study showed reduced mortality and hospitalization due to heart failure (HF) in diabetic patients treated with empagliflozin. Overexpression Ca2+ -dependent activation of /calmodulin-dependent kinase II (CaMKII) are hallmarks HF, leading contractile dysfunction arrhythmias. We tested whether empagliflozin reduces CaMKII- activity improves -handling human murine ventricular myocytes.Myocytes from wild-type mice, mice transverse aortic constriction (TAC) as a model failing...
Adult cardiac tissue undergoes a rapid process of dedifferentiation when cultured outside the body. The in vivo environment, particularly constant electromechanical stimulation, is fundamental to regulation structure and function. We investigated role stimulation preventing culture-induced adult using rat, rabbit human heart failure myocardial slices. Here we report that application preload equivalent sarcomere length (SL) = 2.2 μm optimal for maintenance rat slice structural, functional...
Preclinical testing of cardiotoxicity and efficacy novel heart failure therapies faces a major limitation: the lack an in situ culture system that emulates complexity human tissue maintains viability functionality for prolonged time. To develop reliable, easily reproducible, medium-throughput method to pig slices under physiological conditions period Here, we describe novel, biomimetic (300 µm thickness) 6 days culture. We optimized medium with continuous electrical stimulation at 1.2 Hz...
Abstract Cardiovascular diseases represent a major cause of morbidity and mortality, necessitating research to improve diagnostics, discover test novel preventive curative therapies, all which warrant experimental models that recapitulate human disease. The translation basic science results clinical practice is challenging task, in particular for complex conditions such as cardiovascular diseases, often result from multiple risk factors comorbidities. This difficulty might lead some...
Abstract Aims Cardiac fibroblasts (CFs) are considered the principal regulators of cardiac fibrosis. Factors that influence CF activity difficult to determine. When isolated and cultured in vitro, CFs undergo rapid phenotypic changes including increased expression α-SMA. Here we describe a new model study their response pharmacological mechanical stimuli using vitro mouse, dog human myocardial slices. Methods results Unloading slices induced proliferation without α-SMA up 7 days culture....
Engineered heart tissue (EHT) strategies, by combining cells within a hydrogel matrix, may be novel therapy for failure. EHTs restore cardiac function in rodent injury models, but more data are needed clinically relevant settings. Accordingly, an upscaled EHT patch (2.5 cm × 1.5 mm) consisting of up to 20 million human induced pluripotent stem cell–derived cardiomyocytes (hPSC-CMs) embedded fibrin-based was developed. A rabbit myocardial infarction model then established test feasibility and...
Cardiac remodelling is the process by which heart adapts to its environment. Mechanical load a major driver of remodelling. tissue culture has been frequently employed for in vitro studies load-induced remodelling; however, current protocols (e.g. cyclic stretch, isometric load, and auxotonic load) are oversimplified do not accurately capture dynamic sequence mechanical conformational changes experienced vivo. This limits translational scope relevance findings.We developed novel methodology...
Several pathologic conditions of the heart lead to cardiac structural remodelling. Given high density and opaque nature myocardium, deep three dimensional (3D) imaging is difficult achieve analysis pathological myocardial structure often limited two images thin sections. Efficient methods obtain optical clearing tissue for 3D visualisation are therefore needed. Here we describe a rapid, simple versatile Free-of-Acrylamide SDS-based Tissue Clearing (FASTClear) protocol specifically designed...
Heart function relies on the interplay of several specialized cell types and a precisely regulated network chemical mechanical stimuli. Over last few decades, this complexity has often been undervalued progress in translational cardiovascular research significantly hindered by lack appropriate models. The data collected are oversimplified these make translation results from laboratory to clinical trials challenging occasionally misleading. Living myocardial slices ultrathin (100-400μm)...
Heart failure (HF) is a burgeoning health problem worldwide. Often arising as result of cardiac injury, HF has become major cause mortality with limited availability effective treatments. Ferroptotic pathways, triggering an iron-dependent form cell death, are known to be potential key players in heart disease. This death does not exhibit typical characteristics programmed and mediated by impaired iron metabolism lipid peroxidation signalling.
Cardiosphere-derived cells (CDCs), which can be isolated from heart explants, are a promising candidate cell source for infarcted myocardium regeneration. However, current protocols used to expand CDCs require at least 1 month in vitro obtain sufficient transplantation. We report that CDC culture optimized by preconditioning the under hypoxia (2% oxygen), may reflect physiological oxygen level of stem niche. Under hypoxia, proliferation rate increased 1.4-fold, generating 6 × 10 with higher...
ABSTRACT Introduction Percutaneous left ventricular assist devices (pLVADs) have become essential tools during coronary reperfusion in high risk PCI. Significant reduction infarct propagation is observed when mechanical unloading coupled with reperfusion, but little known of the effect this wall stress and extracellular matrix pLVADs on hearts regenerative capacity. Objective This study investigates has myocardial fibrosis, impact these changes potential. Methods MI was induced by artery...
Abstract The contact cardiac electrogram is derived from the extracellular manifestation of cellular action potentials and cell-to-cell communication. It used to guide catheter based clinical procedures. Theoretically, potential are directly related, should change in conjunction with each other during arrhythmogenesis, however there currently no methodology by which concurrently record both electrograms same preparation for direct validation their relationships mechanistic links. We report a...