A5089878829- Barrier Structure and Function Studies
- Intracerebral and Subarachnoid Hemorrhage Research
- Acute Ischemic Stroke Management
- Drug Transport and Resistance Mechanisms
- Cancer Cells and Metastasis
- Amino Acid Enzymes and Metabolism
- Glycosylation and Glycoproteins Research
- Angiogenesis and VEGF in Cancer
- Nanoparticle-Based Drug Delivery
- Metabolism and Genetic Disorders
- Brain Metastases and Treatment
- Glioma Diagnosis and Treatment
- Clusterin in disease pathology
- Proteoglycans and glycosaminoglycans research
- Machine Learning in Bioinformatics
- Neurological Disease Mechanisms and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- S100 Proteins and Annexins
- Alzheimer's disease research and treatments
- 3D Printing in Biomedical Research
- Innovative Teaching Methods
- MRI in cancer diagnosis
- Nerve injury and regeneration
- Interdisciplinary Research and Collaboration
- Microfluidic and Capillary Electrophoresis Applications
Université d'Artois
2014-2025
Laboratoire Jean Perrin
2009-2024
Université de Lille
2009-2016
Université Lille Nord de France
2009-2016
Predict (France)
2013
Institut de Médecine et d'Epidémiologie Africaines
2013
University of Münster
2012
The migration of polymorphonuclear granulocytes (PMN) into the brain parenchyma and release their abundant proteases are considered main causes neuronal cell death reperfusion injury following ischemia. Yet, therapies targeting PMN egress have been largely ineffective. To address this discrepancy we investigated temporo-spatial localization PMNs early after transient ischemia in a murine middle cerebral artery occlusion (tMCAO) model human stroke specimens. Using specific markers that...
The disappointing clinical outcomes of neuroprotectants challenge the relevance preclinical stroke models and data in defining early cerebrovascular events as potential therapeutic targets. kinetics blood–brain barrier (BBB) leakage after reperfusion link with parenchymal lesion remain debated. By using vivo vitro approaches, we conducted a kinetic analysis BBB dysfunction during reperfusion. After 60 minutes middle cerebral artery occlusion followed by times up to 24 hours mice,...
Amyloid-β (Aβ) accumulation in Alzheimer's disease (AD) and cerebral amyloid angiopathy (CAA) is likely caused by the impairment of its brain clearance that partly occurs through blood-brain barrier (BBB). In this context, an vitro BBB model a valuable tool for studying molecular mech anisms regulate process. This study assessed Aβ elimination across modulation natural chaperones Apolipoprotein A1 (ApoA1) J/Clusterin (ApoJ). The was based on primary endothelial cells were cultured...
The ST6GALNAC5 gene that encodes an α2,6-sialyltransferase involved in the biosynthesis of α-series gangliosides, was previously identified as one genes mediate breast cancer metastasis to brain. We have shown expression MDA-MB-231 cells resulted GD1α ganglioside at cell surface. By using a human blood-brain barrier vitro model recently developed, consisting CD34+ derived endothelial co-cultivated with pericytes, we show decreased interactions between and barrier.
Abstract Background Pediatric diffuse intrinsic pontine glioma (DIPG) represents one of the most devastating and lethal brain tumors in children with a median survival 12 months. The high mortality rate can be explained by ineligibility patients to surgical resection due growth pattern midline localization tumor. While therapeutic strategies are unfortunately palliative, blood–brain barrier (BBB) is suspected responsible for treatment inefficiency. Located at capillary endothelial cells...
The development of effective neurological treatments remains a challenge due to the low permeation across blood-brain barrier (BBB). use nanoparticles for brain drug delivery is promising, but level BBB crossing low, typically around 0.5-3%. We report synthesis negatively charged N-isopropylacrylamide nanogels (diameter 10 nm) displaying in vitro human model nearly 5-fold higher than previously reported, and permeating parenchyma healthy zebrafish with fully formed BBB....
That promising neuroprotectants failed to demonstrate benefit against stroke highlights the great difficulties translate preclinical pharmacological effects in clinical outcomes. Part of this hurdle implies complex response injury neurovascular unit increasing cerebrovascular permeability at level blood-brain barrier (BBB). Previous studies reported neuroprotection animal models upon activation nuclear receptor PPARα (peroxisome proliferator-activated receptor) α, but cellular targets BBB...
Around 7–17% of metastatic breast cancer patients will develop brain metastases, associated with a poor prognosis. To reach the parenchyma, cells need to cross highly restrictive endothelium Blood-Brain Barrier (BBB). As treatments for metastases are mostly inefficient, preventing could provide relevant and important strategy. For that purpose an in vitro approach is required identify cellular molecular interaction mechanisms between BBB endothelium, notably at early steps interaction....
Abstract Background Cell-based therapeutic strategies have been proposed as an alternative for brain repair after stroke, but their clinical application has hampered by potential adverse effects in the long term. The present study was designed to test effect of secretome endothelial progenitor cells (EPCs) from stroke patients (scCM) on vitro human models angiogenesis and vascular barrier. Methods Two different scCM batches were analysed mass spectrometry a proteome profiler. Human primary...
Owing to its ability generate the clot-dissolving protease plasmin, tissue plasminogen activator (tPA) is only approved drug for acute treatment of ischemic stroke. However, tPA also promotes hemorrhagic transformation and excitotoxic events. High mobility group box-1 protein (HMGB-1) a non-histone transcription factor pro-inflammatory cytokine, which has been shown bind both plasminogen. We thus investigated cellular molecular effects through HMGB-1 could influence vascular parenchymal...
α-Series gangliosides define a particular sub-class of glycosphingolipids containing sialic acid α2,6-linked to GalNAc residue that was isolated as minor compound from the brain. The sialyltransferase ST6GalNAc V cloned mouse brain and showed α2,6-sialyltransferase activity almost exclusively for GM1b, form GD1α is considered main enzyme involved in biosynthesis α-series gangliosides. Recently, ST6GALNAC5 identified one genes over-expressed breast cancer cell populations selected their...
Abstract Background CD44 is a multifunctional membrane glycoprotein. Through its heparan sulfate chain, presents growth factors to their receptors. We have shown that and Tropomyosin kinase A (TrkA) form complex following nerve factor (NGF) induction. Our study aimed understand how TrkA interact the consequences of inhibiting this interaction regarding pro-tumoral effect NGF in breast cancer. Methods After determining which isoforms (variants) are involved forming TrkA/CD44 using proximity...
Treatment for CNS related diseases are limited by the difficulty of drugs to cross blood-brain barrier (BBB). The functionalization polymeric nanoparticles (NPs) coated with surfactants polysorbate 80 (PS80) and poloxamer 188 (P188), have shown promising results as carriers able BBB on animal models. In this study, poly(lactide-co-glycolide) (PLGA) NPs PS80 P188, labelled a fluorescent dye were tested human pre-clinical in vitro model evaluate compare their uptake profiles, mechanisms...
Abstract Background Triple-Negative Breast Cancer is particularly aggressive, and its metastasis to the brain has a significant psychological impact on patients' quality of life, in addition reducing survival. The development metastases harmful triple-negative breast cancer (TNBC). To date, mechanisms that induce TNBC are poorly understood. Methods Using human blood–brain barrier (BBB) vitro model, an 3D organotypic extracellular matrix, ex vivo mouse slices co-culture xenograft experiment,...
Ce retour d’expérience présente la mise en œuvre de l’approche par compétences (APC) dans le programme des Sciences Vie. Un travail pionnier a été réalisé pour les expérimentations destinées aux étudiants troisième année licence. Plusieurs ateliers ont conçus aider à s'approprier référentiel compétences, situer place du portfolio l’évaluation développement et s’auto-évaluer. L’évaluation l’APC permis recueillir leurs perceptions. Les exprimé difficultés démarche mais reconnu efforts l'équipe...
The delivery of drugs to the brain remains a challenge due blood-brain barrier's (BBB) highly specific and restrictive properties, which controls restrict access parenchyma. However, with development nanotechnologies, large panels new nanomaterials were developed improve drug delivery, highlighting need for reliable in vitro microsystems predict penetration frame preclinical assays. Here is straightforward method set up microphysiological system model BBB using solely human cells. In its...
This study describes feedback on the effects of changes introduced in our teaching practices for an introductory biochemistry course Life Sciences curriculum. Students this have diverse educational qualifications and are taught large learning groups, creating challenges management individual learning. We used constructive alignment principle, refining contract re-drafting program to introduce active organization activities that promotes participation all students helps their understanding....
Abstract Cell-based therapeutic strategies have been proposed as an alternative for brain and blood vessels repair after stroke, but their clinical application is hampered by potential adverse effects. We therefore tested the hypothesis that secretome of these cells might be used instead to still focus on cell-based strategies. characterized composition effect microvascular endothelial (BMECs) primary in vitro human models angiogenesis vascular barrier. Two different batches produced high...