A5045198746- Microbial Fuel Cells and Bioremediation
- Microbial Community Ecology and Physiology
- Microbial bioremediation and biosurfactants
- Pesticide and Herbicide Environmental Studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Wastewater Treatment and Nitrogen Removal
- Anaerobic Digestion and Biogas Production
- Methane Hydrates and Related Phenomena
- Radioactive element chemistry and processing
- Geochemistry and Elemental Analysis
- Bacterial Genetics and Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Spectroscopy Techniques in Biomedical and Chemical Research
- Hydrocarbon exploration and reservoir analysis
- Enhanced Oil Recovery Techniques
- Toxic Organic Pollutants Impact
- Biosensors and Analytical Detection
- CO2 Sequestration and Geologic Interactions
- Enzyme-mediated dye degradation
- Spectroscopy and Chemometric Analyses
- Environmental Toxicology and Ecotoxicology
Helmholtz Zentrum München
2014-2023
Institute of Groundwater Ecology
2014-2022
Abstract Cable bacteria of the family Desulfobulbaceae couple spatially separated sulfur oxidation and oxygen or nitrate reduction by long-distance electron transfer, which can constitute dominant process in shallow sediments. However, it remains unknown how cells anoxic part centimeter-long filaments conserve energy. We found 16S rRNA gene sequences similar to groundwater cable a 1-methylnaphthalene-degrading culture (1MN). Cultivation with elemental thiosulfate ferrihydrite as acceptors...
Raman microspectroscopy is a prime tool to characterize the molecular and isotopic composition of microbial cells. However, low sensitivity long acquisition times limit broad applicability method in environmental analysis. In this study, we explore potential, applicability, limitations stable isotope (SIRM), resonance SIRM, SIRM combination with surface-enhanced scattering (SERS) for characterization single bacterial The latter two techniques have potential significantly increase decrease...
Exploring adaptive strategies by which microorganisms function and survive in low-energy natural environments remains a grand goal of microbiology, may help address prime challenge the 21st century: degradation man-made chemicals at low concentrations ("micropollutants"). Here we explore physiological adaptation maintenance energy requirements herbicide (atrazine)-degrading microorganism (Arthrobacter aurescens TC1) while concomitantly observing mass transfer limitations directly...
Biodegradation of persistent micropollutants like pesticides often slows down at low concentrations (μg/L) in the environment. Mass transfer limitations or physiological adaptation are debated to be responsible. Although promising, evidence from compound-specific isotope fractionation analysis (CSIA) remains unexplored for bacteria adapted this concentration regime. We accomplished CSIA degradation a pesticide, atrazine, during cultivation Arthrobacter aurescens TC1 chemostat under four...
An anaerobic culture (1MN) was enriched with 1-methylnaphthalene as sole source of carbon and electrons Fe(OH)3 electron acceptor. 1-Naphthoic acid produced a metabolite during growth while 2-naphthoic detected naphthalene 2-methylnaphthalene. This indicates that the degradation pathway might differ from 2-methylnaphthalene in sulfate reducers. Terminal restriction fragment length polymorphism pyrosequencing revealed is mainly composed two bacteria related to uncultured Gram-positive...
For microorganisms that play an important role in bioremediation, the adaptation to swift changes availability of various substrates is a key for survival. The iron-reducing bacterium Geobacter metallireducens was hypothesized repress utilization less preferred presence high concentrations easily degradable compounds. In our experiments, acetate and ethanol were over benzoate, but benzoate co-consumed with toluene butyrate. To reveal overall physiological caused by different single mixture...
The enrichment culture BPL is able to degrade benzene with sulfate as electron acceptor and dominated by an organism of the genus Pelotomaculum. Members Pelotomaculum are usually known be fermenters, undergoing syntrophy anaerobic respiring microorganisms or methanogens. By using a metagenomic approach, we reconstructed high-quality genome (∼2.97 Mbp, 99% completeness) for candidate BPL. proteogenomic data suggested that (1) degradation was activated yet unknown mechanism conversion...
While they are theoretically conceptualized to restrict biodegradation of organic contaminants, bioavailability limitations challenging observe directly. Here we explore the onset mass transfer during slow polycyclic aromatic hydrocarbon 2-methylnaphthalene (2-MN) by anaerobic, sulfate-reducing strain NaphS2. Carbon and hydrogen compound specific isotope fractionation was pronounced at high aqueous 2-MN concentrations (60 μM) (εcarbon = -2.1 ± 0.1‰/εhydrogen -40 7‰) in absence an oil phase...
The strict anaerobe Geobacter metallireducens was cultivated in retentostats under acetate and plus benzoate limitation the presence of Fe(III) citrate order to investigate its physiology close natural conditions. Growth rates below 0.003h(-1) were achieved course cultivation. A nano-liquid chromatography-tandem mass spectrometry-based proteomic approach (nano-LC-MS/MS) with subsequent label-free quantification performed on proteins extracted from cells sampled at different time points...
Slow microbial degradation of organic trace chemicals ("micropollutants") has been attributed to either downregulation enzymatic turnover or rate-limiting substrate supply at low concentrations. In previous biodegradation studies, a drastic decrease in isotope fractionation atrazine revealed transition from enzyme membrane permeation as bottleneck when concentrations fell below the Monod constant growth. With pollutant 4-chlorophenol (4-CP) by Arthrobacter chlorophenolicus A6, this study...
There are two main strategies known how microorganisms regulate substrate utilization: specialization on one preferred at high concentrations in batch cultures or simultaneous utilization of many substrates low chemostats. However, it remains unclear utilize the subsurface: do they focus a single and exhibit catabolite repression de-repress regulation all catabolic pathways? Here, we investigated readiness Geobacter metallireducens to degrade organic under sessile growth sediment columns...
Desulfitobacterium hafniense Y51 has been widely used in investigations of perchloroethylene (PCE) biodegradation, but limited information exists on its other physiological capabilities. We investigated how D. confronts the debilitating limitations not having enough electron donor (lactate), or acceptor (fumarate) during cultivation chemostats. The residual concentrations substrates supplied excess were much lower than expected. Transcriptomics, proteomics and fluxomics integrated to...