A5021782353- Occupational and environmental lung diseases
- Stress Responses and Cortisol
- Pleural and Pulmonary Diseases
- Neonatal Respiratory Health Research
- Cancer Research and Treatments
- Single-cell and spatial transcriptomics
- Lung Cancer Diagnosis and Treatment
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Hormonal Regulation and Hypertension
- Adrenal Hormones and Disorders
- MicroRNA in disease regulation
- Tryptophan and brain disorders
- Cancer-related molecular mechanisms research
- Neuroendocrine regulation and behavior
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Bioinformatics and Genomic Networks
- Neurogenesis and neuroplasticity mechanisms
- Cancer Cells and Metastasis
- Neonatal and fetal brain pathology
- Lung Cancer Research Studies
- Birth, Development, and Health
- Cancer, Stress, Anesthesia, and Immune Response
- Lung Cancer Treatments and Mutations
- Pneumothorax, Barotrauma, Emphysema
Max Planck Institute of Psychiatry
2020-2025
University of Patras
2016-2023
Helmholtz Zentrum München
2021
General University Hospital of Patras
2020
Ludwig-Maximilians-Universität München
2020
Icahn School of Medicine at Mount Sinai
2020
LMU Klinikum
2020
Abstract Malignant pleural effusion (MPE) is the lethal consequence of various human cancers metastatic to cavity. However, mechanisms responsible for development MPE are still obscure. Here we show that mutant KRAS important induction in mice. Pleural disseminated, bearing tumour cells upregulate and systemically release chemokine ligand 2 (CCL2) into bloodstream mobilize myeloid from host bone marrow space via spleen. These promote formation, as indicated by splenectomy splenocyte...
Glucocorticoids are important for proper organ maturation, and their levels tightly regulated during development. Here, we use human cerebral organoids mice to study the cell-type-specific effects of glucocorticoids on neurogenesis. We show that increase a specific type basal progenitors (co-expressing PAX6 EOMES) has been shown contribute cortical expansion in gyrified species. This effect is mediated via transcription factor ZBTB16 leads increased production neurons. A phenome-wide...
A fine-tuned balance of glucocorticoid receptor (GR) activation is essential for organ formation, with disturbances influencing many health outcomes. In utero, glucocorticoids have been linked to brain-related negative outcomes, unclear underlying mechanisms, especially regarding cell-type-specific effects. An in vitro model fetal human brain development, induced pluripotent stem cell (hiPSC)-derived cerebral organoids, was used test whether organoids are suitable studying the impact...
Exposure to stressful life events increases the risk for psychiatric disorders. Mechanistic insight into genetic factors moderating impact of stress can increase our understanding disease processes. Here, we test 3,662 single nucleotide polymorphisms (SNPs) from preselected expression quantitative trait loci in massively parallel reporter assays identify variants that modulate activity regulatory elements sensitive glucocorticoids, important mediators response. Of tested SNP sequences, 547...
During brain development, neural stem cells (NSCs) must balance self-renewal with differentiation and ensure lineage progression. To identify novel regulators of NSCs during neurogenesis, we isolated by FACS from the mouse cerebral cortex ganglionic eminence at mid-neurogenesis, birth, when gliogenesis starts in both, but neurogenesis only continues latter region. RNA-seq ATAC-seq revealed major transcriptional chromatin changes between these stages identified TGFB-Induced Homeobox Factor 2...
Disruptions in the tightly regulated process of human brain development have been linked to increased risk for and mental illnesses. While genetic contribution these diseases is well established, important environmental factors less studied at molecular cellular levels. Here, we used single-cell cell type-specific techniques investigate effect glucocorticoid (GC) exposure, a mediator antenatal risk, on gene regulation lineage specification unguided neural organoids. We characterized...
Disruptions in the tightly regulated process of human brain development have been linked to increased risk for and mental illnesses. While genetic contribution these diseases is well established, important environmental factors less studied at molecular cellular levels. In this study, we used single-cell cell-type-specific techniques investigate effect glucocorticoid (GC) exposure, a mediator antenatal risk, on gene regulation lineage specification unguided neural organoids. We characterized...
Research Article24 March 2017Open Access Source DataTransparent process NRAS destines tumor cells to the lungs Anastasios D Giannou Laboratory for Molecular Respiratory Carcinogenesis, Department of Physiology, Faculty Medicine, University Patras, Rio, Greece Search more papers by this author Antonia Marazioti Nikolaos I Kanellakis orcid.org/0000-0002-0065-2282 Ioanna Giopanou Ioannis Lilis Dimitra E Zazara Giannoula Ntaliarda Danai Kati Vasileios Armenis Georgia A Giotopoulou Anthi C...
Malignant pleural effusion (MPE) is a frequent metastatic manifestation of human cancers. While we previously identified KRAS mutations as molecular culprits MPE formation, the underlying mechanism remained unknown. Here, determine that non-canonical IKKα-RelB pathway activation KRAS-mutant tumor cells mediates development and this fueled by host-provided interleukin IL-1β. Indeed, IKKα required for MPE-competence activating NF-κB signaling. IL-1β fuels addiction mutant to resulting in...
Malignant pleural mesothelioma (MPM) arises from mesothelial cells lining the cavity of asbestos-exposed individuals and rapidly leads to death. MPM harbors loss-of-function mutations in BAP1, NF2, CDKN2A, TP53, but isolated deletion these genes alone mice does not cause mouse models disease are sparse. Here, we show that a proportion human harbor point mutations, copy number alterations, overexpression KRAS with or without TP53 changes. These likely pathogenic, since ectopic expression mutant
Genome-wide gene expression analyses are invaluable tools for studying biological and disease processes, allowing a hypothesis-free comparison of profiles. Traditionally, transcriptomic analysis has focused on gene-level effects found by differential expression. In recent years, network emerged as an important additional level investigation, providing information molecular connectivity, especially diseases associated with large number linked smaller magnitude, like neuropsychiatric...
Objective: Oxidative stress plays an important role in the pathogenesis of diabetic retinopathy.The aim present study was to investigate effect Crocus sativus L. styles (saffron) extract on oxidative indices retina streptozotocin (STZ)induced rats.Methods: Adult male Wistar rats (n=20) were randomized into following 4 groups (n=6-7/ group): Control group (C): normal, + Saffron (CS): non-diabetic treated with 60 mg/ kg saffron extract, Diabetic (D) and (DS): extract.We determined activity...
Pleural effusions frequently signal disseminated cancer. Diagnostic markers of pleural malignancy at presentation that would assess cancer risk and streamline diagnostic decisions remain unidentified. A consecutive cohort 323 patients with effusion (PE) from different etiologies were recruited between 2013 2017 was retrospectively analyzed. Data included history, chest X-ray, blood/pleural fluid cell counts biochemistry. Group comparison, receiver-operator characteristics, unsupervised...
Abstract A fine-tuned balance of glucocorticoid receptor (GR) activation is essential for organ formation, with disturbances influencing health outcomes. Excess GR-activation in utero has been linked to brain-related negative outcomes, unclear underlying mechanisms, especially regarding cell-type specific effects. To address this, we used an vitro model fetal human brain, induced pluripotent-stem-cell-derived cerebral organoids, and mapped effects using single-cell transcriptomics across...
Abstract Genome-wide gene expression analyses are invaluable tools for increasing our knowledge of biological and disease processes, allowing a hypothesis-free comparison profiles across experimental groups, tissues cell types. Traditionally, transcriptomic data analysis has focused on gene-level effects found by differential expression. In recent years, network emerged as an important additional level investigation, providing information molecular connectivity, especially diseases...
ABSTRACT Importance Pleural effusions frequently signal disseminated cancer. Diagnostic markers of pleural malignancy at presentation that would assess cancer risk and streamline diagnostic decisions remain unidentified. Objective The present study aimed identifying validating predictors malignant effusion patient presentation. DESIGN, SETTING, AND PARTICIPANTS A consecutive cohort 323 patients with (PE) from different etiologies were recruited between 2013-2017 was retrospectively analyzed....
Summary Glucocorticoids are important for proper organ maturation and their levels tightly regulated during development. Here we use human cerebral organoids mice to study cell-type specific effects of glucocorticoids on neurogenesis. We show that increase a type basal progenitors (co-expressing PAX6 EOMES ) has been shown drive cortical expansion in gyrified species. This effect is mediated via the transcription factor ZBTB16 leads increased production neurons. A phenome-wide mendelian...
<b>Background:</b> Malignant pleural mesothelioma (MPM) is a highly aggressive tumor linked to loss of suppressorsBAP1, NF2, andTRP53and accompanied by malignant effusion (MPE). <b>Aim:</b> To develop novel mouse models MPM with MPE. <b>Methods:</b> Intercrosses conditional mutantKRAS (LoxP-STOP-LoxP.KRASG12D; K) andTRP53-deleted (LoxP.Trp53.LoxP; P) mice received intrapleural adenovirus-Cre. Pleural tumors were molecularly phenotyped and cultured isolate cell lines, which inoculated into...
Malignant pleural mesothelioma (MPM) arises from mesothelial cells lining the cavity of asbestos-exposed individuals and rapidly leads to development effusion death. MPM harbours loss-of-function mutations in genes like BAP1, NF2, CDKN2A , TP53 but isolated deletion these alone mice does not cause mouse models disease are sparse. Here we show that a significant proportion human harbour point copy number alterations KRAS proto-oncogene. These likely pathogenic, since ectopic expression mutant...
Malignant pleural mesothelioma (MPM) is a highly aggressive tumor caused by asbestos. Faithful mouse models of the disease are missing. Our aim was to develop and characterize conditional transplantable model MPM. Conditional mutant <i>KRAS</i>-expressing (<i>LSL.KRAS</i><sup>G12D</sup>; K) <i>TRP53</i>-deleted (<i>Trp53<sup>f/f</sup></i>; P) mice were intercrossed in all possible combinations, followed intrapleural adenovirus-Cre. Efficient widespread recombination verified using dual...
Adolescence is an important period when the brain undergoes many changes. Because changing so much at this time, our experiences can have a huge impact on brain’s health. Positive keep mind healthy, whereas negative lead to mental disorders. To process all information that comes into brain, cells must talk with each other. They communicate via messenger systems. One of these systems called endocannabinoid (eCB) system. The eCB system plays role in changes happen adolescent brain. In article,...