A5027244220- Cellular transport and secretion
- Microtubule and mitosis dynamics
- Erythrocyte Function and Pathophysiology
- Endoplasmic Reticulum Stress and Disease
- Biotin and Related Studies
- Lipid Membrane Structure and Behavior
- Reproductive tract infections research
- Hippo pathway signaling and YAP/TAZ
- Retinal Development and Disorders
- Click Chemistry and Applications
- Molecular Junctions and Nanostructures
- Glycosylation and Glycoproteins Research
- Immune Response and Inflammation
- Calcium signaling and nucleotide metabolism
- Lysosomal Storage Disorders Research
- HIV Research and Treatment
- Photoreceptor and optogenetics research
- Receptor Mechanisms and Signaling
- Monoclonal and Polyclonal Antibodies Research
- Urinary Tract Infections Management
- Neuroscience and Neuropharmacology Research
- Advanced Fluorescence Microscopy Techniques
- Wnt/β-catenin signaling in development and cancer
- Legionella and Acanthamoeba research
- Photosynthetic Processes and Mechanisms
Centre National de la Recherche Scientifique
2015-2024
Institut Curie
2015-2024
Université Paris Sciences et Lettres
2016-2024
Sorbonne Université
2018-2024
Centre National pour la Recherche Scientifique et Technique (CNRST)
2013-2022
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
2020-2021
Laboratoire de Biologie et Modélisation de la Cellule
2019-2020
Sorbonne Paris Cité
2019
Biologie cellulaire et Cancer
2018-2019
Institut Pasteur
2009-2014
Abstract Despite their roles in intercellular communications, the different populations of extracellular vesicles (EVs) and secretion mechanisms are not fully characterized: how to what extent EVs form as intraluminal endocytic compartments (exosomes), or at plasma membrane (PM) (ectosomes) remains unclear. Here we follow intracellular trafficking EV markers CD9 CD63 from endoplasmic reticulum residency compartment, respectively PM late endosomes. We observe transient co-localization both...
Distinct PSD-95 clusters are primary landmarks of postsynaptic densities (PSDs), which specialized membrane regions for synapses. However, the mechanism that defines locations and whether or how they reorganized inside individual dendritic spines remains controversial. Because palmitoylation regulates targeting, we combined a conformation-specific recombinant antibody against palmitoylated with live-cell super-resolution imaging discovered subsynaptic nanodomains composed serve as elementary...
To ensure their homeostasis and sustain differentiated functions, cells continuously transport diverse cargos to various cell compartments in particular the surface. Secreted proteins are transported along intracellular routes from endoplasmic reticulum through Golgi complex before reaching plasma membrane microtubule tracks. Using a synchronized secretion assay, we report here that exocytosis does not occur randomly at surface but on localized hotspots juxtaposed focal adhesions. Although...
ABSTRACT Reactive oxygen species (ROS) are many-faceted compounds involved in cell defense against pathogens, as well signaling. Their involvement the response to infection epithelial cells remains poorly documented. Here, we investigated production of ROS during with Chlamydia trachomatis , a strict intracellular pathogen, HeLa cells. C. induced transient increase level within few hours, followed by return basal 9 hours after infection. At this time point, host enzyme dedicated production,...
Chlamydia trachomatis is an obligate intracellular pathogen responsible for loss of eyesight through trachoma and millions cases annually sexually transmitted diseases. The bacteria develop within a membrane-bounded inclusion. They lack enzymes several biosynthetic pathways, including those to make some phospholipids, exploit their host compensate. Three-dimensional fluorescence microscopy demonstrates that small organelles the host, peroxisomes, are translocated into inclusion found...
Distinct dimeric conformations of the HIV-1 co-receptor CCR5 govern its appearance at cell surface.
Stimulation of cells with epidermal growth factor (EGF) induces internalization and partial degradation the EGF receptor (EGFR) by endo-lysosomal pathway. For continuous cell functioning, EGFR plasma membrane levels are maintained transporting newly synthesized EGFRs to surface. The regulation this process is largely unknown. In study, we find that stimulation specifically increases transport efficiency from endoplasmic reticulum membrane. This coincides an up-regulation inner coat protein...
ABSTRACT The Golgi complex is responsible for processing and sorting of secretory cargos. Microtubules are known to accelerate the transport proteins from endoplasmic reticulum (ER) plasma membrane. However, whether post-Golgi strictly requires microtubules still unclear. Using retention using selective hooks (RUSH) system synchronize trafficking cargos, we show that anterograde tumor necrosis factor (TNF) strongly reduced without microtubules. We two populations elements co-exist in these...
Glucose transporter 4 (GLUT4) is sequestered inside muscle and fat then released by vesicle traffic to the cell surface in response postprandial insulin for blood glucose clearance. Here, we map biogenesis of this GLUT4 pathway humans, which involves clathrin isoform CHC22. We observe that transits through early secretory more slowly than constitutively secreted GLUT1 localize CHC22 ER-to-Golgi intermediate compartment (ERGIC). functions transport from ERGIC, as demonstrated an essential...
Abstract Radial glial (RG) cells are the neural stem of developing neocortex. Apical RG (aRG) can delaminate to generate basal (bRG) cells, a cell type associated with human brain expansion. Here, we report that aRG delamination is regulated by post‐Golgi secretory pathway. Using in situ subcellular live imaging, show transport RAB6+ vesicles occurs toward minus ends microtubules and depends on dynein. We demonstrate apical determinant Crumbs3 (CRB3) also transported Double knockout RAB6A/A'...
The translocation of the protein high mobility group box 1 (HMGB1) from nucleus to cytoplasm and its secretion or passive release through permeabilized plasma membrane, constitutes a major cellular danger signal. Extracellular HMGB1 can interact with pattern recognition receptors stimulate pro-inflammatory immunostimulatory pathways. Here, we developed screening assay identify pharmacological agents endowed releasing properties. For this, took advantage "retention using selective hooks"...
To reach the lysosome, lysosomal membrane proteins (LMPs) are translocated in endoplasmic reticulum after synthesis and then transported to Golgi apparatus. The existence of a direct transport from apparatus endosomes but also an indirect route through plasma has been described. Clathrin adaptor binding motifs contained cytosolic tail LMPs have described as key players their intracellular trafficking. Here we used RUSH assay synchronize biosynthetic multiple LMPs. After exiting apparatus,...
Chlamydiae are Gram-negative, obligate intracellular pathogens that replicate within a membrane-bounded compartment termed an inclusion. Throughout their development, they actively modify the eukaryotic environment. The type III secretion (TTS) system is main process by which bacteria translocate effector proteins into inclusion membrane and host cell cytoplasm. Here we describe family of secreted effectors present in all pathogenic chlamydiae absent environment-related species. It defined...
Dymeclin is a Golgi-associated protein whose deficiency causes Dyggve–Melchior–Clausen syndrome (DMC, MIM #223800), rare recessively inherited spondyloepimetaphyseal dysplasia consistently associated with postnatal microcephaly and intellectual disability. While the skeletal phenotype of DMC patients has been extensively described, very little known about their cerebral anomalies, which result in brain growth defects cognitive dysfunction. We used Dymeclin-deficient mice to determine cause...
The diversity of secretory routes enabled the identification specific inhibitors CCR5 transport and HIV-1 infection.