A5083886652- Photoreceptor and optogenetics research
- Retinal Development and Disorders
- Neurogenesis and neuroplasticity mechanisms
- Cellular transport and secretion
- Microtubule and mitosis dynamics
- Neuroscience and Neural Engineering
- Hippo pathway signaling and YAP/TAZ
- Pluripotent Stem Cells Research
- Photochromic and Fluorescence Chemistry
- Single-cell and spatial transcriptomics
- Neural dynamics and brain function
Institut Curie
2021-2024
Centre National de la Recherche Scientifique
2015-2024
Université Paris Sciences et Lettres
2021-2024
Institut de la Vision
2015-2019
Inserm
2015-2018
Sorbonne Université
2015-2018
Research Article27 September 2016Open Access Transparent process Red-shifted channelrhodopsin stimulation restores light responses in blind mice, macaque retina, and human retina Abhishek Sengupta INSERM, U968, Paris, France Sorbonne Universités, UPMC Univ Paris 06, UMR_S 968, Institut de la Vision, CNRS, UMR_7210, Search for more papers by this author Antoine Chaffiol Emilie Macé Romain Caplette Mélissa Desrosiers Maruša Lampič Valérie Forster Olivier Marre John Y Lin School of Medicine,...
A major challenge in the treatment of retinal degenerative diseases, with transplantation replacement photoreceptors, is difficulty inducing grafted cells to grow and maintain light sensitive outer segments host retina, which depends on proper interaction underlying pigment epithelium (RPE). Here, for an RPE-independent approach, we introduce a hyperpolarizing microbial opsin into photoreceptor precursors from newborn mice, transplant them blind mice lacking layer. These...
Abstract The human neocortex has undergone strong evolutionary expansion, largely due to an increased progenitor population, the basal radial glial cells. These cells are responsible for production of a diversity cell types, but successive fate decisions taken by individual progenitors remain unknown. Here we developed semi-automated live/fixed correlative imaging method map division modes in early fetal tissue and cerebral organoids. Through live analysis hundreds dividing progenitors, show...
Abstract Radial glial (RG) cells are the neural stem of developing neocortex. Apical RG (aRG) can delaminate to generate basal (bRG) cells, a cell type associated with human brain expansion. Here, we report that aRG delamination is regulated by post‐Golgi secretory pathway. Using in situ subcellular live imaging, show transport RAB6+ vesicles occurs toward minus ends microtubules and depends on dynein. We demonstrate apical determinant Crumbs3 (CRB3) also transported Double knockout RAB6A/A'...
Primary microcephaly and megalencephaly are severe brain malformations defined by reduced increased size, respectively. Whether these two pathologies arise from related alterations at the molecular level is unclear. Microcephaly has been largely associated with centrosomal defects, leading to cell death. Here, we investigate consequences of WDR81 loss function, which causes in patients. We show that regulates endosomal trafficking EGFR function leads MAP kinase pathway activation. Mouse...
Abstract The human neocortex has undergone strong evolutionary expansion, largely due to an increased progenitor population, the basal radial glial (bRG) cells. These cells are responsible for production of a diversity cell types, but successive fate decisions taken by individual progenitors remains unknown. Here, we developed semi-automated live/fixed correlative imaging method generate map bRG division modes in early fetal tissue and cerebral organoids. Through analysis over 1,000 dividing...
A major challenge in the treatment of retinal degenerative diseases, with transplantation replacement photoreceptors, is difficulty inducing grafted cells to grow and maintain light sensitive outer segments (OS) host retina, which depends on proper interaction underlying pigment epithelium (RPE). For a RPE-independent approach, we introduced hyperpolarizing microbial opsin into photoreceptor precursors from new-born mice, transplanted them blind mice lacking layer. These optogenetically...
Abstract Radial glial (RG) cells are the neural stem of developing neocortex. Apical RG (aRG) can delaminate to generate basal (bRG) cells, a cell type associated with human brain expansion. Here, we report that this delamination is regulated by post-Golgi secretory pathway. Using in situ subcellular live imaging, show transport RAB6+ vesicles occurs toward minus ends microtubules and depends on dynein. We demonstrate apical determinant Crumbs3 (CRB3) also transported Double knockout...