A5071946381- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Cell death mechanisms and regulation
- Nanoparticle-Based Drug Delivery
- Mesoporous Materials and Catalysis
- Supramolecular Self-Assembly in Materials
- ATP Synthase and ATPases Research
- Cancer Immunotherapy and Biomarkers
- Porphyrin and Phthalocyanine Chemistry
- Chemical Synthesis and Analysis
- Cancer, Hypoxia, and Metabolism
- Nanoparticles: synthesis and applications
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Gut microbiota and health
- Immunotherapy and Immune Responses
- Cancer-related Molecular Pathways
- Advanced Nanomaterials in Catalysis
- Mitochondrial Function and Pathology
- Protein Degradation and Inhibitors
- Supramolecular Chemistry and Complexes
- CAR-T cell therapy research
- RNA modifications and cancer
- Microbial infections and disease research
- Immune Cell Function and Interaction
Josep Carreras Leukaemia Research Institute
2023-2024
CIBBIM-Nanomedicine
2023
Vall d'Hebron Hospital Universitari
2023
Institut Català de Nanociència i Nanotecnologia
2022-2023
Consejo Superior de Investigaciones Científicas
2023
Vall d'Hebron Institut de Recerca
2022-2023
Université Paris-Saclay
2018-2021
Inserm
2009-2020
Université Paris-Sud
2018-2020
Institut Gustave Roussy
2016-2020
Immune checkpoint inhibitors (ICIs) targeting the PD-1/PD-L1 axis induce sustained clinical responses in a sizable minority of cancer patients. We found that primary resistance to ICIs can be attributed abnormal gut microbiome composition. Antibiotics inhibited benefit patients with advanced cancer. Fecal microbiota transplantation (FMT) from who responded into germ-free or antibiotic-treated mice ameliorated antitumor effects PD-1 blockade, whereas FMT nonresponding failed do so....
The synthesis of new capped silica mesoporous nanoparticles for on-command delivery applications is described. gate-like functional hybrid systems consisted nanoscopic MCM-41-based materials functionalized on the pore outlets with different "saccharide" derivatives and a dye contained in mesopores. A series hydrolyzed starch products as saccharides were selected. S1, S2, S3 containing grafted Glucidex 47, Gludicex 39, 29 synthesized. Additionally, comparative purposes solid S4 lactose was...
Mitophagy is an evolutionarily conserved process that selectively targets impaired mitochondria for degradation. Defects in mitophagy are often associated with diverse pathologies, including cancer. Because the main known regulators of frequently inactivated cancer cells, mechanisms regulate cells not fully understood. Here, we identified E3 ubiquitin ligase (ARIH1/HHARI) triggers a PINK1-dependent manner. We found ARIH1/HHARI polyubiquitinates damaged mitochondria, leading to their removal...
Gated community: Peptides anchored to the surface of silica mesoporous supports by a valid procedure act as gatekeepers. In this way, "zero release" that selectively deliver cargo in presence suitable peptidase are obtained (see picture, red spheres: cargo, colored chains: peptides).
Trapped: Mesoporous silica nanoparticles were loaded with a fluorescent guest and functionalized octadecyltrimethoxysilane. The alkyl chains interact paraffins, which build hydrophobic layer around the particle (see picture). Upon melting of paraffin, molecule is released, as demonstrated in cells for doxorubicin. release temperature can be tuned by choosing an appropriate paraffin.
Learning to let go with age: Intracellular controlled release of molecules within senescent cells was achieved using mesoporous silica nanoparticles (MSNs) capped a galacto-oligosaccharide (GOS) contain the cargo (magenta spheres; see scheme). The GOS is substrate biomarker, senescence-associated β-galactosidase (SA-β-gal), and releases upon entry into SA-β-gal expressing cells. Detailed facts importance specialist readers are published as "Supporting Information". Such documents...
Abstract Hormone receptor (HR) + breast cancer (BC) causes most BC-related deaths, calling for improved therapeutic approaches. Despite expectations, immune checkpoint blockers (ICBs) are poorly active in patients with HR BC, part reflecting the lack of preclinical models that recapitulate disease progression immunocompetent hosts. We demonstrate mammary tumors driven by medroxyprogesterone acetate (M) and 7,12-dimethylbenz[a]anthracene (D) several key features human luminal B HER2 −...
Cancer is a major and still increasing cause of death in humans. Most cancer cells have fundamentally different metabolic profile from that normal tissue. This shift away mitochondrial ATP synthesis via oxidative phosphorylation towards high rate glycolysis, termed Warburg effect, has long been recognized as paradigmatic hallmark cancer, supporting the increased biosynthetic demands tumor cells. Here we show deletion apoptosis-inducing factor (AIF) KrasG12D-driven mouse lung model resulted...
Abstract The synthesis and characterization of two new capped silica mesoporous nanoparticles for controlled delivery purposes are described. Capped hybrid systems consist MCM‐41 functionalized on the outer surface with polymer ε‐poly‐ L ‐lysine by different anchoring strategies. In both cases, were loaded model dye molecule [Ru(bipy) 3 ] 2+ . An strategy involved random formation urea bonds treatment propyl isocyanate‐functionalized lysine amino groups located backbone (solid Ru‐rLys‐S1 )....
Please release me: Mesoporous silica nanoparticles capped with bulky organic moieties containing amide and urea linkages were able to their cargo upon addition of amidase urease. Amidase induced an immediate, yet not complete, the cargo. On other hand, urease allowed a near total that was delayed in time (see graph).
Changes in the conformation of a peptide anchored onto external surface MCM-41 nanoparticles are used to design temperature-controlled delivery systems.
Abstract New capped silica mesoporous nanoparticles for intracellular controlled cargo release within cathepsin B expressing cells are described. Nanometric MCM‐41 supports loaded with safranin O ( S1‐P ) or doxorubicin S2‐P containing a molecular gate based on target peptidic sequence were synthesized. Solids designed to show “zero delivery” and display in the presence of enzyme, which selectively hydrolyzed vitro capping peptide sequence. Controlled delivery HeLa, MEFs WT, lacking cell...
Nanoparticle (NP) pharmacokinetics significantly differ from traditional small molecule principles. From this emerges the need to create new tools and concepts harness their full potential avoid unnecessary risks. strongly depend on size, shape, surface functionalisation, aggregation state, influencing biodistribution, accumulation, transformations, excretion profile, hence efficacy safety. Today, while NP biodistribution nanoceria have been studied often at short times, long-term...
The programmed cell death or apoptosis plays both physiological and pathological roles in biology. Anomalous activation of has been associated with malignancies. intrinsic mitochondrial pathway occurs through a multiprotein complex named the apoptosome. We have discovered molecules that bind to central protein component apoptosome, Apaf-1, inhibits its activity. These new first-in-class apoptosome inhibitors further improved by modifications directed enhance their cellular penetration yield...
Come and gate it: DNA-capped mesoporous silica nanoparticles loaded with a dye are used to detect the common contaminate Mycoplasma in real contaminated cell-culture media without needing polymerase chain reaction (PCR) techniques, at detection limit range of 70 DNA genome copies μL−1. As service our authors readers, this journal provides supporting information supplied by authors. Such materials peer reviewed may be re-organized for online delivery, but not copy-edited or typeset. Technical...
The treatment of breast cancer largely depends on the utilization immunogenic chemotherapeutics, which, as a common leitmotif, stimulate exposure calreticulin (CALR) surface cells, thereby facilitating their recognition by dendritic cells for uptake tumor-associated antigens and subsequent antigen cross-presentation to cytotoxic T cells. Breast also express antagonist CD47, which inhibits tumor cell phagocytosis consequently subverts anticancer immune responses. Here, we treated...
Inhibition of oxidative phosphorylation (OXPHOS) by 1-cyclopropyl-4-(4-[(5-methyl-3-(3-[4-(trifluoromethoxy)phenyl]-1,2,4-oxadiazol-5-yl)-1H-pyrazol-1-yl)methyl]pyridin-2-yl)piperazine (BAY87-2243, abbreviated as B87), a complex I inhibitor, fails to kill human cancer cells in vitro. Driven this consideration, we attempted identify agents that engage synthetically lethal interactions with B87. Here, report dimethyl α-ketoglutarate (DMKG), cell-permeable precursor lacks toxicity on its own,...