A5075454998- Water Treatment and Disinfection
- Toxic Organic Pollutants Impact
- Analytical chemistry methods development
- Lichen and fungal ecology
- Microbial bioremediation and biosurfactants
- Environmental Chemistry and Analysis
- Mycorrhizal Fungi and Plant Interactions
- Receptor Mechanisms and Signaling
- Radioactive contamination and transfer
- Legume Nitrogen Fixing Symbiosis
- Fungal Biology and Applications
- Chemical Analysis and Environmental Impact
- Circadian rhythm and melatonin
- Microbial Community Ecology and Physiology
- Bacterial biofilms and quorum sensing
- Heavy metals in environment
- Neurobiology and Insect Physiology Research
- Radioactive element chemistry and processing
- Smart Materials for Construction
- Soil Carbon and Nitrogen Dynamics
- Plant Stress Responses and Tolerance
- Neuroscience and Neuropharmacology Research
- Mass Spectrometry Techniques and Applications
- Plant responses to elevated CO2
- Plant-Microbe Interactions and Immunity
Czech Academy of Sciences
2001-2022
Czech Academy of Sciences, Institute of Experimental Botany
2005-2022
Czech Academy of Sciences, Institute of Microbiology
2009-2022
Charles University
2004-2009
T. G. Masaryk Water Research Institute
2009
Arbuscular mycorrhizal (AM) symbiosis is heavily and positively implicated in phosphorus (P) acquisition from soil to plants, including many important agricultural crops. Its role plant nitrogen (N) nutrition generally not as prominent or beneficial, with exception of some situations when N available predominantly organic forms. Yet the AM fungi (AMF) are, due their poor exo-enzymatic repertoire, unlikely degrade compounds on own, therefore they possibly depend other microorganisms liberate...
Summary Bacteria communicate with each other in a population density‐dependent process known as quorum sensing. N ‐acyl‐homoserine lactones ( HSL s) are the autoinducers of G ram‐negative bacteria and best‐studied sensing signals so far. s induce various responses plants, including systemic resistance root development. Here, we used different methods, tritium labelling, sensor strain assays monoclonal antibodies (m A bs), to analyse uptake translocation C 8‐ 10‐ homoserine into barley H...
Chloride, which comes into the forest ecosystem largely from sea as aerosol (and has been in past assumed to be inert), causes chlorination of soil organic matter. Studies showed that content organically bound chlorine temperate soils is higher than chloride, and various chlorinated compounds are produced. Our study matter fermentation horizon using radioisotope 36Cl tracer techniques shows microbial clearly prevails over abiotic, being enzymatically mediated proportional chloride time....
Autoradiography helps to determine the distribution and density of muscarinic receptor (MR) binding sites in brain. However, it relies on selectivity radioligands towards their target. 3H-Pirenzepine is commonly believed label predominantly M1MR, 3H-AFDX-384 considered as M2MR selective ligand. Here we performed series autoradiographies with (2 nM), 3H-pirenzepine (5 nM) WT, M1KO, M2KO M4KO mice address ligand selectivity. Labeling using brain sections showed high M1MR. Selectivity varies...
GC-MS identification of W(CO) 6 and OsO 4 confirms these products photochemical vapor generation from W( vi ) Os( iv in reductive oxidative media, respectively.
Trichloroacetic acid (TCA) belongs to secondary atmospheric pollutants affecting the forest health. Distribution of [1,2-14C]TCA-residues and TCA biodegradation were investigated in 4-year-old nursery-grown trees Norway spruce [Picea abies (L.) Karst.] whole plant/soil system. Radioactivity was monitored needles, wood, roots soil as well air. During two weeks exposure continuously degraded, especially soil. Estimates radioactivity balance showed loss into atmosphere form 14CO2;...
Chlorine - one of the most widespread elements on Earth is present in environment as chloride ion or bound to organic substances. The main source ions oceans while organically chlorine (OCl) comes from various sources, including anthropogenic ones. Chlorinated compounds were long considered be only industrial products; nevertheless, organochlorines occur plentifully natural ecosystems. However, recent investigations temperate and boreal forest ecosystems have shown them products...
Winter deicing and traffic spreads salt to road-adjacent Norway spruce trees in the form of spraying slops. Our use Na36Cl revealed roots as main pathway uptake. One-shot application a concentrated solution saplings by both irrigation causes macroscopic damage needles affects needle phyllosphere. Irrigation more than because Cl- uptake through is faster eventually leads higher chloride content plant. Along with root-needle route, spray-deposited from re-transported back into soil again taken...
Chlorine, one of the most abundant elements in nature, undergoes a complex biogeochemical cycle environment, involved formation volatile organochlorines (VOCls), which turn can contribute to environmental problems, contaminate natural ecosystems, and are public health concern. Several industrial sources VOCls have already been identified; however, data - particularly on still scarce. The aim this study was investigate diversity emission from soil several undergrowth plants collected...
Through employing [14C]-PER exposure experiments it was shown for the first time that PER taken up by drought-stressed spruce needles via air/needle pathway is preferably degraded to trichloroacetic acid (TCA) in chloroplasts. TCA formed oxidative biotransformation mineralised CO2 and HCl various degradation routes. contributes increased proton concentration chloroplast, inducing a pH shift leading pathophysiological effect on H+ transport from thylakoid interior into stroma. As result of...
Mice are nocturnal animals. Surprisingly, the majority of physiological/pharmacological studies performed in morning, i.e., non-active phase their diurnal cycle. We compared effects scopolamine, oxotremorine, and cocaine on locomotor response, hypothermia spontaneous behaviour open field arena morning (9:00 AM) evening PM) C57Bl/6NTac wild type mice (WT) lacking M4 muscarinic receptors (M4KO). Furthermore, we also studied vs. densities muscarinic, GABAA, D1-like, D2-like, NMDA kainate using...
Abstract Objectives M 4 muscarinic receptors ( MR ) presumably play a role in motor coordination. Previous studies have shown different results depending on genetic background and number of backcrosses. However, no attention has been given to biorhythms. Material Methods We therefore analyzed biorhythms under light/dark cycle obtained telemetrically intact animals (activity, body temperature) KO mice growth the C57Bl6 using ChronosFit software. Studying pure effects gene knockout daily...
Trichloroacetic acid (TCA), a secondary atmospheric pollutant, is also formed in forest soil and thus ranked among natural organohalogens. The observed biooxidation of tetrachloroethene (PER) to TCA chloroplasts has led the investigation mode action spruce needles, since accumulated needles after its rapid uptake from by roots. Being phytotoxic, considerably influences conifers affecting their photosynthetic apparatus. We examined transport into order compare effects on both sources, i.e....