A5092280129- Developmental Biology and Gene Regulation
- Pluripotent Stem Cells Research
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Neurogenesis and neuroplasticity mechanisms
- CRISPR and Genetic Engineering
- Planarian Biology and Electrostimulation
Institute of Science and Technology Austria
2023-2024
During neural tube (NT) development, the notochord induces an organizer, floorplate, which secretes Sonic Hedgehog (SHH) to pattern progenitors. Conversely, NT organoids (NTOs) from embryonic stem cells (ESCs) spontaneously form floorplates without notochord, demonstrating that can self-organize inducers. Here, we investigated floorplate self-organization in clonal mouse NTOs. Expression of marker FOXA2 was initially spatially scattered before resolving into multiple clusters, underwent...
Despite its fundamental importance for development, the question of how organs achieve their correct size and shape is poorly understood. This complex process requires coordination between generation cell mass morphogenetic mechanisms that sculpt tissues. These processes are regulated by morphogen signalling pathways mechanical forces. Yet, in many systems, it unclear biochemical quantitatively interpreted to determine behaviours individual cells they contribute growth morphogenesis at...
Abstract Developing tissues interpret dynamic changes in morphogen activity to generate cell type diversity. To quantitatively study BMP signalling dynamics the vertebrate neural tube, we developed a new ES differentiation system tailored for growing tissues. Differentiating cells form striking self-organised patterns of dorsal tube types driven by sequential phases that are observed both vitro and vivo. Data-driven biophysical modelling showed these result from coupling fast negative...
Abstract The neural tube (NT) has been a hallmark example of embryonic induction and patterning whereby the notochord induces an organiser, floorplate, that secretes Sonic Hedgehog (SHH) to pattern surrounding field progenitors. On other hand, NT organoids (NTOs) formed from stem cells (ESCs) undergo spontaneous floorplate formation in absence their normal inducers. Understanding how regulative organiser is central challenge biology. Here, we investigated self-organisation SHH-expressing...
Abstract Stem cell differentiation with controlled geometry results in reproducible pattern formation. In contrast to constraining differentiating cells on micropatterned surfaces, we initialise colony formation using elastomeric stencils that adhere culture dishes and create microwells defined sizes shapes. After formation, are removed allow growth migration. Stencil fabrication involves mould production by photolithography followed replica-moulding polydimethylsiloxane (PDMS). This...