A5014854886- Cervical Cancer and HPV Research
- Cancer Genomics and Diagnostics
- Virus-based gene therapy research
- Molecular Biology Techniques and Applications
- Autophagy in Disease and Therapy
- RNA Research and Splicing
- Endoplasmic Reticulum Stress and Disease
- Plant tissue culture and regeneration
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- Cancer and Skin Lesions
- Cancer-related gene regulation
- Renal and related cancers
- Calcium signaling and nucleotide metabolism
- Nonmelanoma Skin Cancer Studies
- Advanced Proteomics Techniques and Applications
- Plant Molecular Biology Research
- Receptor Mechanisms and Signaling
- Zebrafish Biomedical Research Applications
- Machine Learning in Bioinformatics
- Plant Virus Research Studies
- ATP Synthase and ATPases Research
- Seed Germination and Physiology
- Bacteriophages and microbial interactions
- Muscle Physiology and Disorders
Gregor Mendel Institute of Molecular Plant Biology
2014-2024
Austrian Academy of Sciences
2019-2024
Research Institute of Molecular Pathology
2009-2024
Vienna Biocenter
2019-2023
Institute of Molecular Biotechnology
2009-2022
Vienna BioCenter Core Facilities
2019
University of Vienna
2009
Eukaryotes have evolved various quality control mechanisms to promote proteostasis in the endoplasmic reticulum (ER). Selective removal of certain ER domains via autophagy (termed as ER-phagy) has emerged a major mechanism. However, degree which ER-phagy is employed by other branches ER-quality remains largely elusive. Here, we identify cytosolic protein, C53, that specifically recruited autophagosomes during ER-stress, both plant and mammalian cells. C53 interacts with ATG8 distinct binding...
The development of quantitative techniques in mass spectrometry has generated the ability to systematically monitor protein expression. Isobaric tags for relative and absolute quantification (iTRAQ) have become a widely used tool proteins. However, application iTRAQ methodology using ion traps hybrid spectrometers containing an trap such as LTQ-Orbitrap was not possible until pulsed Q dissociation (PQD) higher energy C-trap (HCD). Both methods allow iTRAQ-based on but are less suited...
Abstract The skin is exposed to viral pathogens, but whether they contribute the oncogenesis of cancers has not been systematically explored. Here we investigated 19 tumor types by analyzing off-target reads from commonly available next-generation sequencing data for pathogens. We identified human papillomavirus 42 (HPV42) in 96% (n = 45/47) digital papillary adenocarcinoma (DPA), an aggressive cancer occurring on fingers and toes. show that HPV42, so far considered a nononcogenic,...
During development of the human cerebral cortex, multipotent neural progenitors generate excitatory neurons and glial cells. Investigations transcriptome epigenome have revealed important gene regulatory networks underlying this crucial developmental event. However, posttranscriptional control expression protein abundance during corticogenesis remains poorly understood. We addressed issue by using telencephalic brain organoids grown a dual reporter cell line to isolate performed class...
Abstract Maternal mRNAs are essential for protein synthesis during oogenesis and early embryogenesis. To adapt translation to specific needs development, maternal translationally repressed by shortening the polyA tails. While mRNA deadenylation is associated with decapping degradation in somatic cells, short tails stable. Here we report that germline-specific eIF4E paralog, eIF4E1b, zebrafish oogenesis. eIF4E1b localizes P-bodies embryos binds reported or no tails, including histone mRNAs....
During postnatal murine maturation, behavioral patterns emerge and become shaped by experience-dependent adaptations. the same period, morphology of dendritic spines, morphological correlates excitatory synapses, is known to change, there evidence concurrent alterations synaptosomal protein machinery. To obtain comprehensive quantitative insights in developmental regulation proteome we prepared cortical fractions from a total 16 individual juvenile adult mouse brains (age 3 or 8 weeks,...
Summary Mitochondrial ATP production is essential for development, yet the mechanisms underlying continuous increase in mitochondrial activity during embryogenesis remain elusive. Using zebrafish as a model system vertebrate we comprehensively profile activity, morphology, metabolome, proteome and phospho-proteome well respiratory chain enzymatic activity. Our data show that does not require biogenesis, limited by metabolic substrates at early stages, occurs without an abundance of complexes...
The development of the neuromuscular synapse depends on signaling processes that involve protein phosphorylation as a crucial regulatory event. Muscle-specific kinase (MuSK) is key molecule at whose activity required for formation mature and functional synapse. However, cascade downstream MuSK regulation different components are still poorly understood. In this study we used quantitative phosphoproteomics approach to events their temporal MuSK. We identified total 10,183 phosphopeptides,...
Abstract Maternal mRNAs are essential for protein synthesis during oogenesis and early embryogenesis. To adapt translation to specific needs development, maternal translationally repressed by shortening the polyA tails. While mRNA deadenylation is associated with decapping degradation in somatic cells, short tails stable. Here we report an role germline-specific paralog of cap-binding factor eIF4E, known as eIF4E1b, storage repression eIF4E1b binds cap targeted ribonucleoprotein complexes...
Abstract RNA viruses co-opt the host endomembrane system and organelles to build replication complexes for infection. How responds these membrane perturbations is poorly understood. Here, we explore autophagic response of Arabidopsis thaliana three that hijack different cellular compartments. Autophagy significantly induced within systemically infected tissues, its disruption rendering plants highly sensitive Contrary being an antiviral defense mechanism as previously suggested, quantitative...
During development of the human cerebral cortex, multipotent neural progenitors generate excitatory neurons and glial cells. Investigations transcriptome epigenome have revealed important gene regulatory networks underlying this crucial developmental event. However, post-transcriptional control expression protein abundance during corticogenesis remains poorly understood. We addressed issue by using telencephalic brain organoids grown a dual reporter cell line to isolate performed class...
Abstract Somatic cells acclimate to changes in the environment by temporary reprogramming. Much has been learned about transcription factors that induce these cell-state switches both plants and animals, but how rapidly modulate their proteome remains elusive. Here, we show rapid induction of autophagy during reprogramming triggered phytohormones, immune danger signals. Quantitative proteomics following sequential revealed is required for timely decay previous cellular states tweaking new...
<div>Abstract<p>The skin is exposed to viral pathogens, but whether they contribute the oncogenesis of cancers has not been systematically explored. Here we investigated 19 tumor types by analyzing off-target reads from commonly available next-generation sequencing data for pathogens. We identified human papillomavirus 42 (HPV42) in 96% (<i>n</i> = 45/47) digital papillary adenocarcinoma (DPA), an aggressive cancer occurring on fingers and toes. show that HPV42, so...
<p>Supplementary data depicted in Supplementary Figures S1-S9</p>
<div>Abstract<p>The skin is exposed to viral pathogens, but whether they contribute the oncogenesis of cancers has not been systematically explored. Here we investigated 19 tumor types by analyzing off-target reads from commonly available next-generation sequencing data for pathogens. We identified human papillomavirus 42 (HPV42) in 96% (<i>n</i> = 45/47) digital papillary adenocarcinoma (DPA), an aggressive cancer occurring on fingers and toes. show that HPV42, so...
<p>Supplementary data depicted in Supplementary Figures S1-S9</p>