A5071273115- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Renal and related cancers
- Cancer-related gene regulation
- Biosensors and Analytical Detection
- RNA Research and Splicing
- COVID-19 diagnosis using AI
- Bacteriophages and microbial interactions
- PI3K/AKT/mTOR signaling in cancer
- Cancer Cells and Metastasis
- Mitochondrial Function and Pathology
- Respiratory viral infections research
- ATP Synthase and ATPases Research
- CRISPR and Genetic Engineering
- Zebrafish Biomedical Research Applications
- Colorectal Cancer Treatments and Studies
- COVID-19 Clinical Research Studies
Research Institute of Molecular Pathology
2023-2024
Medical University of Vienna
2023
Institute of Molecular Biotechnology
2020-2021
The COVID-19 pandemic has demonstrated the need for massively-parallel, cost-effective tests monitoring viral spread. Here we present SARSeq, saliva analysis by RNA sequencing, a method to detect SARS-CoV-2 and other respiratory viruses on tens of thousands samples in parallel. SARSeq relies next generation sequencing multiple amplicons generated multiplexed RT-PCR reaction. Two-dimensional, unique dual indexing, using four indices per sample, enables unambiguous scalable assignment reads...
Abstract Maternal mRNAs are essential for protein synthesis during oogenesis and early embryogenesis. To adapt translation to specific needs development, maternal translationally repressed by shortening the polyA tails. While mRNA deadenylation is associated with decapping degradation in somatic cells, short tails stable. Here we report that germline-specific eIF4E paralog, eIF4E1b, zebrafish oogenesis. eIF4E1b localizes P-bodies embryos binds reported or no tails, including histone mRNAs....
Colorectal cancer (CRC) is the second deadliest in world. Besides APC and p53 alterations, PI3K/AKT/MTOR MAPK pathway are most commonly mutated CRC. So far, no treatment options targeting these pathways available routine clinics for CRC patients. We systematically analyzed response of cells to combination small molecular inhibitors PI3K pathways. used 2D, 3D spheroid, collagen gel cultures freshly isolated organoids drug studies. Readout was spheroid or organoid growth, outgrowth, metabolic...
During a pandemic, mitigation as well protection of system-critical or vulnerable institutions requires massively parallel, yet cost-effective testing to monitor the spread agents such current SARS-CoV2 virus. Here we present SARSeq, saliva analysis by RNA sequencing , an approach presence and other respiratory viruses performed on tens thousands samples in parallel. SARSeq is based next generation multiple amplicons generated parallel multiplexed RT-PCR reaction. It relies two-dimensional...
Summary Mitochondrial ATP production is essential for development, yet the mechanisms underlying continuous increase in mitochondrial activity during embryogenesis remain elusive. Using zebrafish as a model system vertebrate we comprehensively profile activity, morphology, metabolome, proteome and phospho-proteome well respiratory chain enzymatic activity. Our data show that does not require biogenesis, limited by metabolic substrates at early stages, occurs without an abundance of complexes...
Abstract SARS-CoV-2 has evolved rapidly towards higher infectivity and partial immune escape over the course of pandemic. This evolution is driven by enormous virus population, that infected close to 200 million people now. Therefore, cost effective scalable methods are needed monitor viral globally. Mutation-specific PCR approaches have become inadequate distinguish variety circulating variants unable detect novel ones. Conversely, whole genome sequencing protocols remain too labor-...
Abstract Maternal mRNAs are essential for protein synthesis during oogenesis and early embryogenesis. To adapt translation to specific needs development, maternal translationally repressed by shortening the polyA tails. While mRNA deadenylation is associated with decapping degradation in somatic cells, short tails stable. Here we report an role germline-specific paralog of cap-binding factor eIF4E, known as eIF4E1b, storage repression eIF4E1b binds cap targeted ribonucleoprotein complexes...
Background The COVID-19 pandemic was largely driven by genetic mutations of SARS-CoV-2, leading in some instances to enhanced infectiousness the virus or its capacity evade host immune system. To closely monitor SARS-CoV-2 evolution and resulting variants at genomic-level, an innovative pipeline termed SARSeq developed Austria. Aim We discuss technical aspects pipeline, describe performance present noteworthy results it enabled during Methods set up as a collaboration between private public...