A5008505598- Nuclear Structure and Function
- Cellular transport and secretion
- Bacteriophages and microbial interactions
- Polyomavirus and related diseases
- Biochemical and Molecular Research
- Peptidase Inhibition and Analysis
- Virus-based gene therapy research
- DNA Repair Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- RNA Research and Splicing
- Advanced Proteomics Techniques and Applications
- Cancer-related Molecular Pathways
- Ubiquitin and proteasome pathways
- SARS-CoV-2 detection and testing
- Neurological disorders and treatments
- PI3K/AKT/mTOR signaling in cancer
- Bioinformatics and Genomic Networks
- Genomics and Chromatin Dynamics
- Autophagy in Disease and Therapy
- Biosensors and Analytical Detection
- Calcium signaling and nucleotide metabolism
- Protein Tyrosine Phosphatases
- Antenna Design and Analysis
- COVID-19 diagnosis using AI
- Enzyme Structure and Function
Medical University of Vienna
2001-2021
Max Perutz Labs
2010-2021
Vienna Biocenter
1997-2021
St Anna Children's Hospital
2010
The University of Sydney
2003-2005
Westmead Institute
2005
Westmead Hospital
2005
University of Vienna
1997-1999
Impairment of protein phosphatases, including the family serine/threonine phosphatases designated PP2A, is essential for pathogenesis many diseases, cancer. The ability PP2A to dephosphorylate hundreds proteins regulated by over 40 specificity-determining regulatory "B" subunits that compete assembly and activation heterogeneous heterotrimers. Here, we reveal how a small molecule, DT-061, specifically stabilizes B56α-PP2A holoenzyme in fully assembled, active state selective substrates, such...
The COVID-19 pandemic has demonstrated the need for massively-parallel, cost-effective tests monitoring viral spread. Here we present SARSeq, saliva analysis by RNA sequencing, a method to detect SARS-CoV-2 and other respiratory viruses on tens of thousands samples in parallel. SARSeq relies next generation sequencing multiple amplicons generated multiplexed RT-PCR reaction. Two-dimensional, unique dual indexing, using four indices per sample, enables unambiguous scalable assignment reads...
The methyl-esterification of the C-terminal leucine protein phosphatase 2A (PP2A) catalytic (C) subunit is essential for assembly specific trimeric PP2A holoenzymes, and this region C also contains two threonine tyrosine phosphorylation sites. Most commercial antibodies-including monoclonal antibody 1D6 that part a frequently used, assay kit-are directed toward terminus subunit, raising questions as to their ability recognize methylated phosphorylated forms enzyme. Here, we tested several...
Recognition of Myc-tagged proteins by the widely used antibody 9E10 varies depending on sequences adjacent to tag.
Lamin A, a protein component of the nuclear lamina, is synthesized as precursor named prelamin whose multi-step maturation process involves different intermediates. As demonstrated in laminopathies such familial partial lipodystrophy, mandibuloacral dysplasia, Werner syndrome, Hutchinson-Gilford progeria syndrome and restrictive dermopathy, failure A processing results accumulation lamin precursors inside nucleus which dominantly produces aberrant chromatin structure. To understand if lamina...
Export of the serotonin transporter (SERT) from endoplasmic reticulum (ER) is mediated by SEC24C isoform coatomer protein-II complex. SERT must enter axonal compartment and reach presynaptic specialization to perform its function, i.e., inward transport serotonin. Refilling vesicles contingent on operation an efficient relay between vesicular monoamine transporter-2 (VMAT2). Here, we visualized distribution both endogenously expressed heterologously variants human in dissociated rat dorsal...
Protein phosphatase 2A (PP2A) is an important regulator of signal transduction pathways and a tumor suppressor. Phosphorylation the PP2A catalytic subunit (PP2AC) at tyrosine 307 has been claimed to inactivate was examined in more than 180 studies using commercial antibodies, but this modification never identified mass spectrometry. Here we show that most cited pTyr307 monoclonal E155 F-8, are not specific for phosphorylated Tyr307 instead hampered by PP2AC methylation leucine 309 or...
The BARD1 gene is mutated in a subset of breast and ovarian cancers, implicating as potential tumor suppressor. gains ubiquitin E3 ligase activity when heterodimerized with BRCA1, but the only known BRCA1-independent function p53-dependent proapoptotic stimulated by nuclear export to cytoplasm. We described previously nuclear-cytoplasmic shuttling BARD1, this study, we identify transport sequences that target nucleus show they are essential for regulation cell cycle. used deletion mapping...
Background Disease-linked missense mutations can alter a protein's function with fatal consequences for the affected individual. How single amino acid substitution in protein affects its properties, is difficult to study context of cellular proteome, because mutant proteins often not be traced cells due lack mutation-specific detection tools. Antibodies, however, their exquisite epitope specificity permit substitutions but are available vast majority disease-causing proteins. One most...
ABSTRACT Although polyomavirus large T antigen readily transactivates S-phase-specific enzymes in serum-starved Swiss 3T3 mouse fibroblasts, it is incapable by itself to efficiently drive such cells into S phase. We describe here that this inability correlates with a weak proficiency of the viral protein induce synthesis cyclin A and E stimulate respective cyclin/cdk activities. Polyomavirus small antigen, which together supports S-phase induction, strongly contributes A. In addition, causes...
Abstract Western blotting is one of the most widely used techniques in molecular biology and biochemistry. Prestained proteins are as weight standards protein electrophoresis. In chemiluminescent blot analysis, however, these colored markers invisible leaving researchers with unsatisfying situation that signal for interest not captured simultaneously have to be merged an error-prone step. To allow simultaneous detection marker we generated monoclonal antibodies specific dyes. elicit a dye...
ABSTRACT Polyomavirus large and small T antigens cooperate in the induction of S phase serum-deprived Swiss 3T3 cells. While antigen is able to induce phase-specific enzymes, we have recently shown that both contribute production cyclins E A essential for cyclin A-dependent cdk2 activity (S. Schüchner E. Wintersberger, J. Virol. 73:9266–9273, 1999). Here present our attempts elucidate mechanisms by which transactivate murine gene. Using cells carrying various mutants thereof under...
Abstract Development of molecularly targeted therapies have shifted our clinical approach to cancer allowing for an integrated treatment where tumor genotype and molecular profiles are paired in a clinically actionable manner with specific therapies. This maximizes efficacy minimizes toxicity each patient. While the vast majority focused on development kinase inhibition, equally effective yet underexplored strategy exists activation or regulation phosphatases. The suppressor Protein...