A5022573604- Neurotransmitter Receptor Influence on Behavior
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Forensic Toxicology and Drug Analysis
- 3D Printing in Biomedical Research
- Hydrogels: synthesis, properties, applications
- Psychedelics and Drug Studies
- Proteoglycans and glycosaminoglycans research
- Cellular transport and secretion
- Nerve injury and regeneration
- Ion channel regulation and function
- Diverse Education and Engineering Focus
- Oral microbiology and periodontitis research
- Botulinum Toxin and Related Neurological Disorders
- Advanced Drug Delivery Systems
- Pesticide Exposure and Toxicity
- Polysaccharides and Plant Cell Walls
- Cardiac, Anesthesia and Surgical Outcomes
- Anesthesia and Neurotoxicity Research
- Neurogenesis and neuroplasticity mechanisms
- Anesthesia and Sedative Agents
- Glycosylation and Glycoproteins Research
- Bipolar Disorder and Treatment
- Career Development and Diversity
- Wound Healing and Treatments
Atlantic Technological University
2022-2024
Technological University Dublin
2022-2024
Athlone Institute of Technology
2021
Medical University of Vienna
2010-2016
University College Dublin
2007-2015
Institute of Pharmacology
2014
Mater Misericordiae University Hospital
2011
Nerve functions require phosphatidylinositol-4,5-bisphosphate (PIP 2 ) that binds to ion channels, thereby controlling their gating. Channel properties are also attributed serotonin transporters (SERTs); however, SERT regulation by PIP has not been reported. SERTs control neurotransmission removing from the extracellular space. An increase in results transporter-mediated efflux triggered amphetamine-like psychostimulants. Herein, we altered abundance of activating phospholipase-C (PLC),...
The high-resolution crystal structure of the leucine transporter (LeuT) is frequently used as a template for homology models dopamine (DAT). Although similar in structure, DAT differs considerably from LeuT number ways: (i) when compared to LeuT, has very long intracellular amino and carboxyl termini; (ii) share rather low overall sequence identity (22%) (iii) extracellular loop 2 (EL2) substantially longer than that LeuT. Extracellular zinc binds restricts transporter's movement through...
Hyaluronic acid (HA) is a naturally occurring mucopolysaccharide that, due to its inherent bioactivity and extracellular matrix-like structure, has the potential be utilised extensively in tissue engineering. However, this glycosaminoglycan lacks properties required for cellular adhesion photo-crosslinking by UV light, which significantly hinders polymers applicability. This research presents method modifying hyaluronic via thiolation methacrylation generate novel photo-crosslinkable polymer...
Peripheral nerve injuries, predominantly affecting individuals aged 20-40, pose significant healthcare challenges, with current surgical methods often failing to achieve complete functional recovery. This study focuses on the development of 3D printed hydrogel conduits using modified hyaluronic acid (HA) for potentially enhancing peripheral regeneration. Hyaluronic was chemically altered cysteamine HCl and methacrylic anhydride create thiolated HA (HA-SH) methacrylated (HA-MA), achieving a...
Export of the serotonin transporter (SERT) from endoplasmic reticulum (ER) is mediated by SEC24C isoform coatomer protein-II complex. SERT must enter axonal compartment and reach presynaptic specialization to perform its function, i.e., inward transport serotonin. Refilling vesicles contingent on operation an efficient relay between vesicular monoamine transporter-2 (VMAT2). Here, we visualized distribution both endogenously expressed heterologously variants human in dissociated rat dorsal...
Serotonergic neurotransmission is terminated by reuptake of extracellular serotonin (5-HT) the high-affinity transporter (SERT). Selective 5-HT inhibitors (SSRIs) such as fluoxetine or escitalopram inhibit SERT and are currently principal treatment for depression anxiety disorders. In addition, a major molecular target psychostimulants cocaine amphetamines. Amphetamine-induced transport reversal at closely related dopamine (DAT) has been shown previously to be contingent upon modulation...
Illegal 'ecstasy' tablets frequently contain 3,4-methylenedioxymethamphetamine (MDMA)-like compounds of unknown pharmacological activity. Since monoamine transporters are one the primary targets MDMA action in brain, a number analogues have been tested for their ability to inhibit [3H]noradrenaline uptake into rat PC12 cells expressing noradrenaline transporter (NET) and [3H]5-HT HEK293 stably transfected with 5-HT (SERT).Concentration-response curves following at both NET SERT were...
Background 3,4-Methylenedioxymethamphetamine (MDMA or ‘ecstasy’) tablets are frequently contaminated by 4-MTA (‘flatliner’), an amphetamine derivative which is known to induce severe human intoxication and even death. Although equipotent inducer of SERT-dependent 5-HT release in vivo, does not MDMAlike serotoninergic neurotoxicity rats. Instead, users typically report unpleasant sympathomimetic effects such as tachycardia, tremors, stomach cramps, headache sweating following ingestion. Here,...
Background Monoamine transporters such as the dopamine transporter (DAT) and serotonin (SERT) mediate reuptake of previously released monoamines (DA) from synaptic cleft; thereby, these regulate monoamine content available for transmission. Certain stimuli, changes in ionic composition extracellular fluid or psychostimulants (e.g. amphetamines) are able to induce outward transport thus increase concentrations. Influx efflux substrate thought be asymmetrical processes regulated by...
Background Neuronal functions, such as excitability or endoand exocytosis, require phosphatidylinositol-4,5-bisphosphate (PIP2) since ion channels and other proteins involved in these processes are regulated by PIP2. Monoamine transporters control neurotransmission removing monoamines from the extracellular space. They also display channel properties, but their regulation PIP2 has not been reported. The psychostimulant amphetamine acts on monoamine to stimulate transportermediated currents...
Background The plasma membrane serotonin transporter (SERT) is a key regulator of synaptic serontonergic neurotransmission and major target both antidepressents psychostimulant drugs abuse. pre-synaptic soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) syntaxin 1A has been reported to modulate the intrinsic activity multiple monoamine neurotransmitter transporters in vitro ex vivo. However, contrast dopamine (DAT) little know its effect on SERT-dependent...
s and Programme: EUROANAESTHESIA 2011: The European Anaesthesiology Congress: Obstetric Anaesthesia