A5068712367- Biomedical and Chemical Research
- Kruppel-like factors research
- CRISPR and Genetic Engineering
- Histone Deacetylase Inhibitors Research
- Fibroblast Growth Factor Research
- Skin and Cellular Biology Research
- Biosensors and Analytical Detection
- Animal Genetics and Reproduction
- Chromosomal and Genetic Variations
- Advanced biosensing and bioanalysis techniques
- Chromium effects and bioremediation
- Hippo pathway signaling and YAP/TAZ
- Pluripotent Stem Cells Research
- COVID-19 diagnosis using AI
- SARS-CoV-2 detection and testing
- Amino Acid Enzymes and Metabolism
- Metabolism and Genetic Disorders
Institute of Molecular Biotechnology
2021
New York University
2019
University of Edinburgh
2018
Edinburgh Cancer Research
2018
MRC Centre for Regenerative Medicine
2018
Deutsche Ophthalmologische Gesellschaft
1972-1973
Springer Nature (Germany)
1971-1972
The COVID-19 pandemic has demonstrated the need for massively-parallel, cost-effective tests monitoring viral spread. Here we present SARSeq, saliva analysis by RNA sequencing, a method to detect SARS-CoV-2 and other respiratory viruses on tens of thousands samples in parallel. SARSeq relies next generation sequencing multiple amplicons generated multiplexed RT-PCR reaction. Two-dimensional, unique dual indexing, using four indices per sample, enables unambiguous scalable assignment reads...
CRISPR/Cas9 can be used for precise genetic knock-in of epitope tags into endogenous genes, simplifying experimental analysis protein function. However, Cas9-assisted tagging in primary mammalian cell cultures is often inefficient and reliant on plasmid-based selection strategies. Here, we demonstrate improved efficiencies diverse (V5, 3XFLAG, Myc, HA) using co-delivery Cas9 pre-complexed with two-part synthetic modified RNAs (annealed crRNA:tracrRNA) single-stranded oligodeoxynucleotide...