A5025267674- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Chronic Lymphocytic Leukemia Research
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Biosensors and Analytical Detection
- T-cell and B-cell Immunology
- Bacteriophages and microbial interactions
- SARS-CoV-2 detection and testing
- Hippo pathway signaling and YAP/TAZ
- Biochemical and Molecular Research
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- COVID-19 diagnosis using AI
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Respiratory viral infections research
- Ubiquitin and proteasome pathways
- Cytomegalovirus and herpesvirus research
- Cancer-related Molecular Pathways
- Signaling Pathways in Disease
St. Anna Children's Cancer Research Institute
2023-2025
St Anna Children's Hospital
2023-2025
Research Institute of Molecular Pathology
2020-2021
Institute of Cell Biophysics
2020
Pushchino Scientific Center for Biological Research
2020
Vienna Biocenter
2020
The COVID-19 pandemic has demonstrated the need for massively-parallel, cost-effective tests monitoring viral spread. Here we present SARSeq, saliva analysis by RNA sequencing, a method to detect SARS-CoV-2 and other respiratory viruses on tens of thousands samples in parallel. SARSeq relies next generation sequencing multiple amplicons generated multiplexed RT-PCR reaction. Two-dimensional, unique dual indexing, using four indices per sample, enables unambiguous scalable assignment reads...
Combinatorial action of transcription factors (TFs) with partially overlapping expression is a widespread strategy to generate novel gene-expression patterns and, thus, cellular diversity. Known mechanisms underlying combinatorial activity require co-expression TFs within the same cell. Here, we describe mechanism by which two that are never co-expressed new, intersectional pattern in C. elegans embryos: lineage-specific priming gene transiently expressed TF generates unique intersection...
<p>Supplementary Figure S7: Analysis of differentially accessible genes in NK cell resistant tumour cells.</p>
<p>Supplementary Figure S8: Secondary resistant B-ALL cell clones.</p>
<p>Supplementary Figure S3: Flow cytometric analyses of human data sets and the NK-cell receptor ligand on mouse B-ALL cell lines.</p>
<p>Supplementary Figure S9: Profiles of tumour cells co-cultured with WT, Prf1-/- or Ifng-/- NK cells.</p>
<div>Abstract<p>The term cancer immunoediting describes the dual role by which immune system can suppress and promote tumor growth is divided into three phases: elimination, equilibrium, escape. The of NK cells has mainly been attributed to elimination phase. Here, we show that play a in all phases immunoediting. Extended co-culturing DNA-barcoded mouse BCR/ABL<sup>p185+</sup> B-cell acute lymphoblastic leukemia (B-ALL) with allowed for quantitative measure...
<p>Supplementary Figure S10: Generation of Plaat3 KO B-ALL clones and their characterisation.</p>
<p>Supplementary Figure S2: Flow cytometric analysis and gating strategies shown in 5.</p>
<p>Supplementary Figure S5: Extended quantification of NK cell-mediated cancer immunoediting <i>in vitro</i>.</p>
<p>Supplementary Figure S6: Analysis of differentially expressed genes in NK cell resistant tumour cells.</p>
<p>Supplementary Figure S1: Flow cytometric analysis and gating strategies.</p>
<p>Supplementary Figure S11: The expression of LY6E on K562 does not impact IFN-γ or TNF-α production in NK cells.</p>
<p>Supplementary Figure S4: B-ALL cell line characterisation and details about the barcode library.</p>
Ewing sarcoma (EwS) is an aggressive cancer of adolescents in need effective treatment. Insulin-like growth factor (IGF)-1 autocrine for EwS, but only 10% patients respond to IGF-1 receptor (IGF-1R) blockade. Although EwS presumed originate from mesenchymal progenitors during bone development, targeting the driver oncogene EWS::FLI1 lineage a mouse model does not result tumor formation skeletal malformations and perinatal death. We report that transient exposure concentrations mimicking...
Horizontally acquired genes are usually transcriptionally inactive, although most of them associated with genomic loci enriched promoter-like sequences forming "promoter islands." We hypothesized that lateral DNA transfer induces local mutagenesis, accumulating AT base pairs and creating sequences, whose occupancy RNA polymerase a specific silencer H-NS suppresses the transcription foreign genes. Error-prone mutagenesis was implemented for island" gene appY promoter region an inherent dps....
During a pandemic, mitigation as well protection of system-critical or vulnerable institutions requires massively parallel, yet cost-effective testing to monitor the spread agents such current SARS-CoV2 virus. Here we present SARSeq, saliva analysis by RNA sequencing , an approach presence and other respiratory viruses performed on tens thousands samples in parallel. SARSeq is based next generation multiple amplicons generated parallel multiplexed RT-PCR reaction. It relies two-dimensional...
The nuclear factor of activated T cells (NFAT) family transcription factors plays central roles in adaptive immunity murine models; however, their contribution to human immune homeostasis remains poorly defined. In a multigenerational pedigree, we identified 3 patients who carry germ line biallelic missense variants NFATC1, presenting with recurrent infections, hypogammaglobulinemia, and decreased antibody responses. compound heterozygous NFATC1 these caused stability reduced the binding DNA...
Abstract Ewing sarcoma (EwS) is an aggressive cancer of adolescents in need effective treatments. Insulin like growth factor (IGF) 1 was previously reported autocrine for EwS, but only 10% patients responded to IGF-1 receptor blockade. Although presumed originate from mesenchymal progenitors during bone development, targeting the EwS driver oncogene EWS::FLI1 lineage a conditional mouse model did not result tumor formation led skeletal malformations and perinatal death. We report that...
Architectural protein Dps of the bacterial nucleoid employs side groups lysines at its N-terminal modules for interacting with sugar-phosphate backbone DNA. Electrostatic nature interaction assumes potential ability to bind any nucleotide sequence including RNA. The available data also indicate that exhibits enhanced affinity branched DNA structures. In RNA molecules such structures are formed more frequently than in Hence, aim this investigation was studying purified immobilized on acrylate...
Аннотация.Функциональные свойства промоторных островков генома кишечной палочки, т.е.участков с аномально высокой плотностью сигналов транскрипции, сопоставлены со свойствами геномных областей, обогащённых А/Т-парами.Установлено, что компьютерные выборки участков обоих типов по соответствующим критериям частично перекрываются, а их функциональные во многом похожи
Abstract The term cancer immunoediting describes the dual role by which immune system can suppress and promote tumour growth is divided into three phases: elimination, equilibrium escape. of NK cells has mainly been attributed to elimination phase. Here we show that play a in all phases immunoediting. Extended co-culturing DNA barcoded mouse BCR/ABLp185+ B-cell acute lymphoblastic leukaemia (B-ALL) with allowed for quantitative measure cell–mediated Although most cell clones were efficiently...
Abstract The term cancer immunoediting describes the dual role by which immune system can suppress and promote tumour growth is divided into three phases: elimination, equilibrium escape. of NK cells has mainly been attributed to elimination phase. Here we show that play a in all phases immunoediting. Extended co-culturing DNA barcoded mouse BCR/ABL p185+ B acute lymphoblastic leukaemia (B-ALL) with allowed for quantitative measure cell-mediated Whereas most cell clones were efficiently...