A5034064177- CAR-T cell therapy research
- Chronic Lymphocytic Leukemia Research
- Immune Cell Function and Interaction
- Acute Lymphoblastic Leukemia research
- Chronic Myeloid Leukemia Treatments
- Acute Myeloid Leukemia Research
- Childhood Cancer Survivors' Quality of Life
- Lymphoma Diagnosis and Treatment
- T-cell and B-cell Immunology
- Infection Control and Ventilation
- Respiratory viral infections research
- SARS-CoV-2 detection and testing
- Genomic variations and chromosomal abnormalities
- Molecular Biology Techniques and Applications
- Cytomegalovirus and herpesvirus research
- Renal and related cancers
- Advanced biosensing and bioanalysis techniques
- Genetics and Neurodevelopmental Disorders
St. Anna Children's Cancer Research Institute
2014-2024
St Anna Children's Hospital
2014-2024
University of Veterinary Medicine Vienna
2023
The mutational landscape of B-cell precursor acute lymphoblastic leukemia (BCP-ALL), the most common pediatric cancer, is not fully described partially because commonly applied short-read next generation sequencing has a limited ability to identify structural variations. By combining comprehensive analysis variants (SVs), single-nucleotide (SNVs), and small insertions-deletions, new subtype-defining therapeutic targets may be detected. We analyzed somatic alterations in 60 patients diagnosed...
<p>Supplementary Figure S7: Analysis of differentially accessible genes in NK cell resistant tumour cells.</p>
<p>Supplementary Figure S8: Secondary resistant B-ALL cell clones.</p>
<p>Supplementary Figure S3: Flow cytometric analyses of human data sets and the NK-cell receptor ligand on mouse B-ALL cell lines.</p>
<p>Supplementary Figure S9: Profiles of tumour cells co-cultured with WT, Prf1-/- or Ifng-/- NK cells.</p>
<div>Abstract<p>The term cancer immunoediting describes the dual role by which immune system can suppress and promote tumor growth is divided into three phases: elimination, equilibrium, escape. The of NK cells has mainly been attributed to elimination phase. Here, we show that play a in all phases immunoediting. Extended co-culturing DNA-barcoded mouse BCR/ABL<sup>p185+</sup> B-cell acute lymphoblastic leukemia (B-ALL) with allowed for quantitative measure...
<p>Supplementary Figure S10: Generation of Plaat3 KO B-ALL clones and their characterisation.</p>
<p>Supplementary Figure S2: Flow cytometric analysis and gating strategies shown in 5.</p>
<p>Supplementary Figure S5: Extended quantification of NK cell-mediated cancer immunoediting <i>in vitro</i>.</p>
<p>Supplementary Figure S6: Analysis of differentially expressed genes in NK cell resistant tumour cells.</p>
<p>Supplementary Figure S1: Flow cytometric analysis and gating strategies.</p>
<p>Supplementary Figure S11: The expression of LY6E on K562 does not impact IFN-γ or TNF-α production in NK cells.</p>
<p>Supplementary Figure S4: B-ALL cell line characterisation and details about the barcode library.</p>
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Chromosome 21 is the most affected chromosome in childhood acute lymphoblastic leukemia. Many of its numerical and structural abnormalities define diagnostically clinically important subgroups. To obtain an overview about their types approximate genetic subgroup-specific incidence distribution, we performed cytogenetic, FISH array analyses a total 578 ALL patients (including 26 with constitutional trisomy 21). The latter preferred method to assess genome-wide large fine-scale copy number...
A heterogeneous genetic subtype of B-cell precursor acute lymphoblastic leukemia is driven by constitutive kinase-activation, including patients with JAK2 fusions. In our study, we model the impact a novel fusion protein on hematopoietic development in human induced pluripotent stem cells (hiPSCs). We insert RUNX1-JAK2 into one endogenous RUNX1 allele through employing trans paired nicking genome editing. Tagging degron facilitates depletion using heterobifunctional compound dTAG-13....
Abstract The term cancer immunoediting describes the dual role by which immune system can suppress and promote tumour growth is divided into three phases: elimination, equilibrium escape. of NK cells has mainly been attributed to elimination phase. Here we show that play a in all phases immunoediting. Extended co-culturing DNA barcoded mouse BCR/ABLp185+ B-cell acute lymphoblastic leukaemia (B-ALL) with allowed for quantitative measure cell–mediated Although most cell clones were efficiently...