A5019664786- Reproductive tract infections research
- Monoclonal and Polyclonal Antibodies Research
- HER2/EGFR in Cancer Research
- Kruppel-like factors research
- Protein purification and stability
- Fibroblast Growth Factor Research
- Bacterial Infections and Vaccines
- Bacteriophages and microbial interactions
- Cervical Cancer and HPV Research
- Pneumonia and Respiratory Infections
- Hepatitis B Virus Studies
- Viral Infectious Diseases and Gene Expression in Insects
- SARS-CoV-2 and COVID-19 Research
- Skin and Cellular Biology Research
- Transgenic Plants and Applications
- Advanced Fluorescence Microscopy Techniques
- thermodynamics and calorimetric analyses
- Histone Deacetylase Inhibitors Research
- vaccines and immunoinformatics approaches
- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Immunotherapy and Immune Responses
- HIV Research and Treatment
- Receptor Mechanisms and Signaling
- Lung Cancer Treatments and Mutations
Lawrence Livermore National Laboratory
2021-2025
New York University
2019-2021
University of California, Davis
2014-2015
University of California Davis Medical Center
2014
Abstract Engineered outer membrane vesicles (OMVs) derived from Gram-negative bacteria are a promising technology for the creation of non-infectious, nanoparticle vaccines against diverse pathogens. However, antigen display on OMVs can be difficult to control and highly variable due bottlenecks in protein expression localization host cell, especially bulky and/or complex antigens. Here, we describe universal approach avidin-based vaccine crosslinking (AvidVax) whereby biotinylated antigens...
The COVID-19 pandemic underscored the promise of monoclonal antibody-based prophylactic and therapeutic drugs
Chloroplasts are critical organelles in plants and algae responsible for accumulating biomass through photosynthetic carbon fixation cellular maintenance metabolism the cell. increasingly appreciated their role biomanufacturing, as they can produce many useful molecules, a deeper understanding of chloroplast regulation function would provide more insight biotechnological applications these organelles. However, traditional genetic approaches to manipulate chloroplasts slow, generation...
Rapid, high throughput measurements of biomolecular interactions are essential across medicine and bioscience. Traditional methods for affinity-screening proteins require a long costly process involving cell-based expression, purification, titration multiple concentrations to arrive at binding curve. In contrast, we have developed fast simple approach that yields wealth information about the expression protein its characteristics, all in "one-pot reaction" done under several hours without...
Receptor tyrosine kinases (RTKs) play critical roles in physiological and pathological processes are important anticancer drug targets. In vitro mechanistic discovery studies of full-length RTKs require protein that is both fully functional free from contaminating proteins. Here we describe a rapid cell-free detergent-free co-translation method for producing ERBB2 EGFR receptor supported by water-soluble apolipoprotein A-I based nanolipoprotein particles.
Chlamydia trachomatis is the most common bacterial sexually transmitted pathogen. The number of chlamydial infections continuous to increase and there an urgent need for a safe efficacious vaccine. To assess ability muridarum polymorphic membrane protein G (PmpG) plasmid glycoprotein 3 (Pgp3) as single antigens, in combination with major outer-membrane (MOMP) induce protection, BALB/c mice were immunized utilizing CpG-1826 Montanide ISA 720 VG adjuvants. Following vaccination MOMP,...
Abstract The mammalian ErbB receptor tyrosine kinase family is critical for the development and maintenance of a variety tissues. Though not completely understood, mechanism activation involves binding ligand to extracellular domain leading conformational change that allows dimerization phosphorylation initiate downstream signaling pathways. Mutations, amplification, aberrant these receptors lead oncogenesis tumor progression in several cancer types, including lung, breast, colon. Current...
Abstract Chlamydia trachomatis is the most prevalent bacterial sexually transmitted pathogen worldwide. Since chlamydial infection largely asymptomatic with potential for serious complications, a preventative vaccine likely viable long-term answer to this public health threat. Cell-free protein synthesis (CFPS) utilizes cellular manufacturing machinery decoupled from requirement maintaining viability, offering flexible, rapid, and de-centralized production of recombinant antigens. Here, we...
Abstract Vaccine development against Chlamydia, a prevalent sexually transmitted infection (STI), is imperative due to its global public health impact. However, significant challenges arise in the production of effective subunit vaccine based on recombinant protein antigens, particularly with membrane proteins like Major Outer Membrane Protein (MOMP). Cell-free synthesis (CFPS) technology an attractive method address these as high-throughput and complex coupled nanolipoprotein particles...
Rapid, high throughput measurements of biomolecular interactions are essential across medicine and bioscience. Traditional methods for affinity-screening proteins require a long costly process involving cell-based expression, purification, titration multiple concentrations to arrive at binding curve. In contrast, we have developed fast simple approach that yields wealth information about the expression protein its characteristics, all in "one-pot reaction" done under several hours without...
is the most prevalent bacterial sexually transmitted pathogen in humans worldwide. Since chlamydial infection largely asymptomatic with potential for serious complications, a preventative vaccine likely viable long-term answer to this public health threat. Cell-free protein synthesis (CFPS) utilizes cellular manufacturing machinery decoupled from requirement maintaining viability, offering flexible, rapid, and decentralized production of recombinant antigens.
Vaccine development against Chlamydia, a prevalent sexually transmitted infection (STI), is imperative due to its global public health impact. However, significant challenges arise in the production of effective subunit vaccines based on recombinant protein antigens, particularly with membrane proteins like Major Outer Membrane Protein (MOMP).
Abstract Engineered outer membrane vesicles (OMVs) derived from laboratory strains of bacteria are a promising technology for the creation non-infectious, nanoparticle vaccines against diverse pathogens. As mimics bacterial cell surface, OMVs offer molecularly-defined architecture programming repetitive, high-density display heterologous antigens in conformations that elicit strong B and T immune responses. However, antigen on surface can be difficult to control highly variable due...
Abstract According to a 2012 study by the U.S. Center for Disease Control and Prevention (CDC), African-American women had 41% higher rate of breast cancer mortality during 2005-2009 than did Caucasian women. Although overall rates have declined significantly in since early 1990s, black-white gap has been steadily growing. The underlying causes this racial disparity are likely diverse, include factors such as lower quality care reduced access mammographic screening. However, more aggressive...
Summary The fate choice between stem cell self-renewal and differentiation is regulated by bistable transcriptional networks, which are balanced in homeostasis imbalanced tumors. Yet, how cells switch from to remains a conundrum. Here, we discover molecular mechanism that allows cell-like tumor propagating (TPCs) squamous carcinomas (SCCs) mutually exclusive SOX2-PITX1-TP63 circuit KLF4 driven program, dependent on the relative occupancy of novel Klf4 -regulatory enhancer cluster ( EC944 )...